Early life microbial exposures shape the Crassostrea gigas immune system for lifelong and intergenerational disease protection
Manon Fallet,Caroline Montagnani,Bruno Petton,Luc Dantan,Julien de Lorgeril,S. Comarmond,Cristian Chaparro,Eve Toulza,Simon Boitard,Jean-Michel Escoubas,Agnès Vergnes,Jacqueline Le Grand,Ingo Bulla,Yannick Gueguen,Jeremie Vidal-Dupiol,Christoph Grunau,Guillaume Mitta,Céline Cosseau +17 more
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The role of the environmental microbial community on immune system maturation and protection from infectious disease is still an open question in the Pacific oyster, Crassostrea gigas as mentioned in this paper .Abstract:
The interaction of organisms with their surrounding microbial communities influences many biological processes, a notable example of which is the shaping of the immune system in early life. In the Pacific oyster, Crassostrea gigas, the role of the environmental microbial community on immune system maturation - and, importantly, protection from infectious disease - is still an open question.Here, we demonstrate that early life microbial exposure durably improves oyster survival when challenged with the pathogen causing Pacific oyster mortality syndrome (POMS), both in the exposed generation and in the subsequent one. Combining microbiota, transcriptomic, genetic, and epigenetic analyses, we show that the microbial exposure induced changes in epigenetic marks and a reprogramming of immune gene expression leading to long-term and intergenerational immune protection against POMS.We anticipate that this protection likely extends to additional pathogens and may prove to be an important new strategy for safeguarding oyster aquaculture efforts from infectious disease. tag the videobyte/videoabstract in this section Video Abstract. read more
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Present and future challenges for the investigation of transgenerational epigenetic inheritance.
Manon Fallet,Mélanie Blanc,Michela Di Criscio,Philipp Antczak,Magnus Engwall,Carlos Guerrero-Bosagna,Joëlle Rüegg,Steffen Keiter +7 more
TL;DR: Transgenerational Epigenetic Inheritance (TEI) as discussed by the authors describes the phenomenon that phenotypic traits, such as changes in fertility, metabolic function, or behavior, induced by environmental factors (e.g., parental care, pathogens, pollutants, climate change), can be transferred to offspring generations via epigenetic mechanisms.
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Impacts of Seawater pH Buffering on the Larval Microbiome and Carry-Over Effects on Later-Life Disease Susceptibility in Pacific Oysters
Clara L. Mackenzie,Christopher M. Pearce,Sarah Leduc,Daniel Roth,Colleen T. E. Kellogg,Rute B. G. Clemente-Carvalho,Timothy J. Green +6 more
TL;DR: In this paper , the authors investigated the impact of pH buffering on the larval microbiome of Pacific oysters (Crassostrea gigas) and found that even minimal buffering results in a changed microbiome which is paralleled by increased mortality of later-life stages under Vibrio and temperature stressors, highlighting the importance of the hatchery environment with regard to shaping resilience to summer mortality events.
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A core of functional complementary bacteria infects oysters in Pacific Oyster Mortality Syndrome
Sudhir Agrawal,Genichiro Ishii +1 more
TL;DR: In this paper , a combination of metabarcoding and metatranscriptomic approaches was used to identify a core bacterial consortium which, together with the herpesvirus OsHV-1 µVar, formed the POMS pathobiota.
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Editorial: Interaction between marine invertebrates and symbiotic microbes in a changing environment: Community structure and ecological functions
TL;DR: Li, Zhang, Sun, Thompson and Zhang as discussed by the authors published an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY), provided the original author(s) and the copyright owners are credited and that the original publication in this journal is cited.
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Colonization order of bacterial isolates on treefrog embryos impacts microbiome structure in tadpoles
TL;DR: Priority effects, or impacts of colonization order, may have lasting influence on ecological community composition in newly hatched tadpoles as mentioned in this paper , which may be especially impactful for embryos developing in bacteria-rich environments, such as the embryos of many amphibians.
References
More filters
Journal ArticleDOI
Moderated estimation of fold change and dispersion for RNA-seq data with DESeq2
TL;DR: This work presents DESeq2, a method for differential analysis of count data, using shrinkage estimation for dispersions and fold changes to improve stability and interpretability of estimates, which enables a more quantitative analysis focused on the strength rather than the mere presence of differential expression.
Journal ArticleDOI
phyloseq: an R package for reproducible interactive analysis and graphics of microbiome census data.
Paul J. McMurdie,Susan Holmes +1 more
TL;DR: The phyloseq project for R is a new open-source software package dedicated to the object-oriented representation and analysis of microbiome census data in R, which supports importing data from a variety of common formats, as well as many analysis techniques.
Journal ArticleDOI
Statistical significance for genomewide studies
John D. Storey,Robert Tibshirani +1 more
TL;DR: This work proposes an approach to measuring statistical significance in genomewide studies based on the concept of the false discovery rate, which offers a sensible balance between the number of true and false positives that is automatically calibrated and easily interpreted.
Journal ArticleDOI
Metagenomic biomarker discovery and explanation
Nicola Segata,Jacques Izard,Jacques Izard,Levi Waldron,Dirk Gevers,Larisa Miropolsky,Wendy S. Garrett,Curtis Huttenhower +7 more
TL;DR: A new method for metagenomic biomarker discovery is described and validates by way of class comparison, tests of biological consistency and effect size estimation to address the challenge of finding organisms, genes, or pathways that consistently explain the differences between two or more microbial communities.
Journal ArticleDOI
Fast and sensitive protein alignment using DIAMOND
TL;DR: DIAMOND is introduced, an open-source algorithm based on double indexing that is 20,000 times faster than BLASTX on short reads and has a similar degree of sensitivity.