Journal ArticleDOI
Effect of pH additive and column temperature on kinetic performance of two different sub-2 μm stationary phases for ultrafast separation of charged analytes.
TLDR
It was found that column performance was strongly dependent on the type of stationary phase, especially in acidic medium, and ammonia acetate at neutral pH led to the best results in terms of both efficiency and peak capacity.About:
This article is published in Journal of Chromatography A.The article was published on 2012-03-09. It has received 19 citations till now. The article focuses on the topics: Ammonium acetate & Ammonium formate.read more
Citations
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Journal ArticleDOI
Kinetic evaluation of new generation of column packed with 1.3 μm core-shell particles.
Szabolcs Fekete,Davy Guillarme +1 more
TL;DR: It was observed that the extra-column band broadening could have a major impact on the apparent kinetic performance of this column, and significant plate count loss was noticed for retention factors of less than 5, even with the best system on the market.
Journal ArticleDOI
On-line comprehensive two-dimensional separations of charged compounds using reversed-phase high performance liquid chromatography and hydrophilic interaction chromatography. Part I: orthogonality and practical peak capacity considerations.
TL;DR: A comparative study of different sets of chromatographic conditions including stationary phase, mobile phase and column temperature was carried out with mixtures of representative solutes in order to find out the best two-dimensional analytical conditions for charged compounds.
Journal ArticleDOI
Optimization of conditions in on-line comprehensive two-dimensional reversed phase liquid chromatography. Experimental comparison with one-dimensional reversed phase liquid chromatography for the separation of peptides
TL;DR: A procedure for RPLC×RPLC separations able to define, for a given analysis time, the optimized LC×LC parameters for achieving the best compromise between high peak capacity and low dilution is proposed.
Journal ArticleDOI
On-line comprehensive two dimensional separations of charged compounds using reversed-phase high performance liquid chromatography and hydrophilic interaction chromatography. Part II: application to the separation of peptides.
Amélie D’Attoma,Sabine Heinisch +1 more
TL;DR: A ten-fold gain in analysis time along with a significant gain in peak capacity are obtained with both systems compared to the most efficient one-dimensional separation of peptides recently published.
Journal ArticleDOI
Kinetic performance comparison of fully and superficially porous particles with sizes ranging between 2.7 μm and 5 μm: Intrinsic evaluation and application to a pharmaceutical test compound
TL;DR: The reintroduction of superficially porous particles has resulted in a leap forward for the separation performance in liquid chromatography, and the underlying reasons for the higher efficiency of columns packed with these particles are discussed.
References
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Book
Analytical profiles of drug substances
TL;DR: In this paper, the authors present the Cumulative Index of Cumulative Histories of Chemical Substances (CHS) for the first time, which is the most complete list.
Journal ArticleDOI
Gradient elution in high-performance liquid chromatography
L.R. Snyder,J.W. Dolan,J.R. Gant +2 more
TL;DR: In this article, a general theory of separation for gradient elution with reversed-phase systems is presented, and expressions for retention, resolution, band width and other separation parameters are presented as a function of experimental variables.
Journal ArticleDOI
Equations for calculation of chromatographic figures of merit for ideal and skewed peaks
Joe P. Foley,John G. Dorsey +1 more
TL;DR: The names, symbols, and general expressions that have evolved for the parameters used in chromatographic peak characterization are presented and it is proposed that these parameters be referred to collectively.
Journal ArticleDOI
Fast, comprehensive two-dimensional liquid chromatography.
TL;DR: This report is deliberately focused on the issues involved in doing fast 2DLC by means of elevating the column temperature; however, many issues of broader applicability will be discussed.
Journal ArticleDOI
Some reflections on speed and efficiency of modern chromatographic methods
TL;DR: In this article, the authors compare various techniques in terms of attainable efficiency and speed as well as of the demands on the detectors, and some speculations on future proliferation will be given.