Open AccessJournal Article
[Endogenous lipid pattern, organ distribution and effect of diet on a fatty acid binding protein fraction of rat liver cytosol].
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TLDR
With immunological techniques it has been shown that the FABP has no organ-specificity and no immunological relation to the lipoproteins of the serum, and a physiological function in the intracellular translocation of the fatty acids in different rat organs was inferred.Abstract:
The endogenous lipid pattern of a fatty acid binding protein (FABP; synonym: "Z"-protein) in the cytosolic fraction of rat liver involves besides "free" fatty acids also phospholipids and mono-, di- and triacylglycerols. The relation between "free" fatty acids and the sum of all other lipids is nearly 1. In consequence of this result a correction of the idea is necessary that the FABP specifically binds only fatty acids. With immunological techniques it has been shown that the FABP has no organ-specificity and no immunological relation to the lipoproteins of the serum. A high content of fat in the diet (25% fat) compared with a low fat content of the diet (1% fat) led to an increase of the concentration of FABP in the cytosols of different rat organs (liver, intestinal mucosa, fat tissue and heart). Therefore a physiological function in the intracellular translocation of the fatty acids in different rat organs was inferred.read more
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Journal ArticleDOI
Bovine fatty acid binding proteins
TL;DR: When a 100000 ×g supernatant from bovine heart was incubated with [1-14C]oleic acid and subjected to isoelectric focusing, two fatty acid binding proteins (FABPs) with isoeLECTric points at 4.9 and 5.1 were detected.
Book ChapterDOI
Intracellular Sterol Binding Proteins: Cholesterol Transport and Membrane Domains
Friedhelm Schroeder,Andrey Frolov,Jonathan Schoer,Adalberto M. Gallegos,Barbara P. Atshaves,Neal J. Stolowich,A. Ian Scott,Ann B. Kier +7 more
TL;DR: Immunocytochemical evidence supports the presence of significant amounts of proteins such as the sterol carrier protein-2 outside of peroxisomes and demonstrates that these cholesterol binding proteins are involved in cholesterol uptake and intracellular trafficking.
Dissertation
Studies on sterol carrier protein
TL;DR: Lipid binding studies indicated that the purified SCP is a fatty acid binding protein, however, no cholesterol binding by SCP was observed.