Journal ArticleDOI
Enzymatic degradation of ribosomal RNA in isolated purified ribosomes.
About:
This article is published in Biochemical and Biophysical Research Communications.The article was published on 1969-06-06. It has received 32 citations till now. The article focuses on the topics: Eukaryotic Ribosome & Ribosome.read more
Citations
More filters
Journal ArticleDOI
Further Studies with Labelled Polypeptide‐Chain‐Initiation Factor 3 (IF‐3)
TL;DR: It is found that the 30-S binding of IF-3 is rather sensitive to aurin-tricarboxylic acid, a powerful inhibitor of polypeptide chain initiation, but relatively insensitive to streptomycin.
Journal ArticleDOI
Effect of edeine on aminoacyl-tRNA binding to ribosomes and its relationship to ribosomal binding sites.
W. Szer,Z. Kurylo-Borowska +1 more
TL;DR: Edeine is capable of removing an aminoacyl-tRNA prebound to a trinucleotide codon, however, an aminoACYl- tRNA pre bound to a hexa- or polynucleotide messenger is resistant to the antibiotic.
Journal ArticleDOI
The integrity of the 25-S ribosomal RNA from Euglena gracilis 87-S ribosomes.
TL;DR: The 25-S RNA molecule seems to be partly cleaved at selected loci in situ; depending on the isolation conditions the 25- S RNA molecule may undergo further degradation during the deproteinization procedure.
Journal ArticleDOI
Interactions of edeine with bacterial ribosomal subunits. Selective inhibition of aminoacyl-tRNA binding sites.
W. Szer,Z. Kurylo-Borowska +1 more
TL;DR: The addition of edeine to a preformed 70-S initiation complex does not prevent completion of the translation thus initiated of phage RNA messenger but it inhibits re-initiation.
Journal ArticleDOI
Association of a ribonuclease with the 50-S ribosomal subunit of Escherichia coli MRE 600
Howard Ceri,P.Y. Maeba +1 more
TL;DR: A protein fraction containing a 50-S protein isolated from rRNA was found to be active in degrading RNA and inhibition of HgCl 2, sodium dodecyl sulfate and alcohols inhibited autodegradation.
References
More filters
Journal ArticleDOI
Molecular characterization of ribonucleic acid from Escherichia coli ribosomes
TL;DR: The ribon nucleic acid from purified 30 s, 50 s and 70 s ribosomes of E. coli has been isolated by the phenol and detergent methods and it is suggested that they are heterogeneous; some contain one 23 s ribonucleic acid component, while others contain two 16 s units.
Journal ArticleDOI
Purification and Properties of Ribonuclease III From Escherichia Coli
TL;DR: A nuclease with specificity for double-stranded RNA (RNase III) has been found in extracts of Escherichia coli and appears to be endonucleolytic, with an absolute requirement for divalent cations and for monovalent cations.
Journal ArticleDOI
Isolation and physical properties of the ribosomal ribonucleic acid of Escherichia coli.
Journal ArticleDOI
Translation of the genetic message. VII. Role of initiation factors in formation of the chain initiation complex with Escherichia coli ribosomes.
TL;DR: Incubation of purified ribosomes with 32 P-labeled Qβ phage RNA and methionine- 14 C-labeling formylmethionyl-transfer RNA, at low magnesium ion concentrations, followed by zonal centrifugation analysis in sucrose gradients showed that formation of the initiation complex involves at least two steps.