Purification and Properties of Ribonuclease III From Escherichia Coli
TLDR
A nuclease with specificity for double-stranded RNA (RNase III) has been found in extracts of Escherichia coli and appears to be endonucleolytic, with an absolute requirement for divalent cations and for monovalent cations.About:
This article is published in Journal of Biological Chemistry.The article was published on 1968-01-10 and is currently open access. It has received 434 citations till now. The article focuses on the topics: RNase PH & Ribonuclease III.read more
Citations
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Methods and compositions for rna interference
TL;DR: In this paper, a hairpin RNA was used to attenuate gene expression in a cell, especially in a mammalian cell, using gene-targeted double stranded RNA (dsRNA).
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Dicing and slicing: the core machinery of the RNA interference pathway.
TL;DR: This review describes the discovery of two ribonuclease machines and discusses future lines of work on this amazing biochemical pathway, which is triggered by double‐stranded RNA.
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A conserved double-stranded RNA-binding domain.
TL;DR: On the basis of the binding studies and computer analysis, a consensus sequence is derived that defines a 65- to 68-amino acid dsRNA-binding domain, the minimal region that binds ds RNA.
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Ribonuclease activity and RNA binding of recombinant human Dicer.
TL;DR: Cl cloning and expression of the 218 kDa human Dicer are reported, and characterization of its ribonuclease activity and dsRNA‐binding properties are described, suggesting that ionic interactions are involved in ds RNA cleavage.
Patent
Oligoribonucleotides and ribonucleases for cleaving RNA
TL;DR: Oligomeric compounds are useful for diagnostics and other research purposes, for modulating the expression of a protein in organisms, and for the diagnosis, detection and treatment of other conditions susceptible to oligonucleotide therapeutics.
References
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Journal Article
Protein Measurement with the Folin Phenol Reagent
TL;DR: Procedures are described for measuring protein in solution or after precipitation with acids or other agents, and for the determination of as little as 0.2 gamma of protein.
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A Method for Determining the Sedimentation Behavior of Enzymes: Application to Protein Mixtures
Robert G. Martin,Bruce N. Ames +1 more
TL;DR: Sucrose gradient centrifugation is found to be a suitable method for determining sedimentation coefficients of enzymes in protein mixtures and the sedimentation behavior of several of the enzymes in the pathway of histidine biosynthesis in S. typhimurium has been determined.
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Molecular Configuration in Sodium Thymonucleate
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The release of enzymes by osmotic shock from Escherichia coli in exponential phase.
Nancy G. Nossal,Leon A. Heppel +1 more
TL;DR: A number of degradative enzymes are specifically released from exponentially growing Escherichia coli by osmotic shock, which includes alkaline phosphatase, cyclic phosphodiesterase, 5'-nucleotidase, acid phosphat enzyme, and the ribonucleic acid-inhibited endonuclease that is active with deoxyribon nucleic acid.
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