Journal ArticleDOI
Enzymes on material surfaces.
Joey N. Talbert,Julie M. Goddard +1 more
TLDR
This review breaks down the immobilized enzyme system to look at the individual components of the system-namely the enzyme, the material, and the interface and identifies areas of future research needed to overcome limitations in the current state-of-the art for immobilization systems.About:
This article is published in Colloids and Surfaces B: Biointerfaces.The article was published on 2012-05-01. It has received 292 citations till now. The article focuses on the topics: Immobilized enzyme & Enzyme Interaction.read more
Citations
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Journal ArticleDOI
Industrial use of immobilized enzymes
TL;DR: The development and attributes of several established and emerging industrial applications for immobilized enzymes, including high-fructose corn syrup production, pectin hydrolysis, debittering of fruit juices, interesterification of food fats and oils, biodiesel production, and carbon dioxide capture are reviewed herein, highlighting factors that define the advantages of enzyme immobilization.
Journal ArticleDOI
Conformational changes of enzymes upon immobilisation
TL;DR: This tutorial review outlines first the most common spectroscopic techniques used for investigating the conformation of immobilized proteins, and then examines how protein loading and polar and hydrophobic/hydrophilic interactions with the carrier affect the structural and dynamic features of enzymes.
Journal ArticleDOI
Protein hydrolysis using proteases: An important tool for food biotechnology
TL;DR: In this present review, some aspects of the processes that involve protein hydrolysis steps are discussed, especially considering the application of specific proteases as a tool on food biotechnology.
Journal ArticleDOI
Functionalized ceramics for biomedical, biotechnological and environmental applications
TL;DR: In this review, surface functionalization strategies for alumina, zirconia, titania, silica, iron oxide and calcium phosphate are presented and discussed and particular challenges regarding surface analysis and characterization are identified.
Journal ArticleDOI
Increasing the activity of immobilized enzymes with nanoparticle conjugation.
TL;DR: Improvements in nanoparticle size and surface chemistry as well as the orientation and density of immobilized enzymes all contribute to the enhanced performance of enzyme-nanoparticle conjugates.
References
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Book
Proteins: Structures and Molecular Properties
TL;DR: This paper discusses the physical properties of polypeptides, the structure of which has been determined Crystallographically to High Resolution and its role in the biosynthesis of Proteins.
Journal ArticleDOI
Improvement of enzyme activity, stability and selectivity via immobilization techniques
Cesar Mateo,Jose M. Palomo,Gloria Fernández-Lorente,Jose M. Guisan,Roberto Fernandez-Lafuente +4 more
TL;DR: In all cases, enzyme engineering via immobilization techniques is perfectly compatible with other chemical or biological approaches to improve enzyme functions and the final success depend on the availability of a wide battery of immobilization protocols.
Journal ArticleDOI
Chemistry for peptide and protein PEGylation.
TL;DR: PEG chemistry and methods of preparation are reviewed with a particular focus on new (second-generation) PEG derivatives, reversible conjugation and PEG structures.
Journal ArticleDOI
Enzyme immobilization: The quest for optimum performance
TL;DR: Different methods for the immobilization of enzymes are critically reviewed, with emphasis on relatively recent developments, such as the use of novel supports, e.g., mesoporous silicas, hydrogels, and smart polymers, novel entrapment methods and cross-linked enzyme aggregates (CLEAs).
Journal ArticleDOI
Polymer surface modification for the attachment of bioactive compounds
TL;DR: This paper reviews recent advances in the covalent attachment of bioactive compounds to functionalized polymer surfaces including relevant techniques in polymer surface modification such as wet chemical, organosilanization, ionized gas treatments, and UV irradiation.