Open AccessJournal Article
Evaluation of curcumin as potential herbal agent for mitigation of nickel and chromium induced micronuclei in human blood cultures
TLDR
Curcumin has a mitigating effect against micronuclei induced by metal salts due to its antioxidant and free radical scavenging properties.Abstract:
Chromium and nickel are widely used industrial chemicals.Welders in India are inclined to possible occupational chromium and nickel exposure. We have investigated the Genotoxicity exerted by nickel (Ni) in the form of nickel chloride (4.216 X 10−5M) and chromium (Cr) in the form of potassium dichromate (1.36 X 106M), alone and in combination and mitigating effect of curcumin (3.87 X 10−7M) was studied at two different exposure intervals (24 and 69 hours) in toxicant added human blood cultures. Evaluation of genotoxicity was done by micronuclei (MN) assay. One thousand binucleate cells per group were analyzed. Statistical analysis was performed by one way ANOVA followed by Dunett's test and percentage amelioration was calculated. Nickel and chromium proved to be more toxic in 69-hours exposure than in 24 hours in induction of micronuclei. Curcumin supplementation exhibited significant (71%-92%) amelioration at both exposure intervals. Hence, curcumin has a mitigating effect against micronuclei induced by metal salts due to its antioxidant and free radical scavenging properties.read more
Citations
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Induction of hepatic portal fibrosis, mitochondria damage, and extracellular vesicle formation in Sprague-Dawley rats exposed to copper, manganese, and mercury, alone and in combination.
TL;DR: It is concluded that Cu, Mn, and Hg alone and as part of a mixture cause cellular damage, inflammation, and fibrosis increasing the risk of associated diseases.
Journal ArticleDOI
Cytogenetic Alteration Induced by Nickel and Chromium in Human Blood Cultures and its Amelioration by Curcumin
TL;DR: Curcumin was proved as a protective agent against Ni and Cr induced genotoxicity at two different exposure intervals with a single dose and the amelioration of this toxicity using curcumin.
Journal ArticleDOI
Ameliorative effects of curcumin towards cyclosporine-induced genotoxic potential: an in vitro and in silico study.
TL;DR: It is anticipated that the induction of antioxidant enzymes by curcumin would activate Nrf2-Keap1 pathway as the plausible mechanism to exert anti-inflammatory response as demonstrated in renal epithelial cells.
Journal ArticleDOI
In Vitro Amelioration by Curcumin on Genotoxicity in Workers with Elevated Blood Cadmium Level
TL;DR: It can be concluded that curcumin which is herbal antidote can be helpful to protect cadmium toxicity in occupationally exposed workers.
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New Giemsa method for the differential staining of sister chromatids
Paul Perry,Sheldon Wolff +1 more
TL;DR: If human lymphocytes1 or Chinese hamster2 cells are treated with the base analogue 5-bromodeoxyuridine in the latter part of the S period, Giemsa stained chromosomes exhibit a pattern of condensed and extended segments along their length, allowing the identification of the two chromatids, and the observation of sister chromatid exchanges (SCEs) without recourse to autoradiography.
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Genotoxicity of chromium compounds. A review.
TL;DR: This article reviews approximately 700 results reported in the literature with 32 chromium compounds assayed in 130 short-term tests, using different targets and/or genetic end-points, to provide useful information for predicting and interpreting the peculiar patterns of Cr(VI) carcinogenicity.
Journal ArticleDOI
Inhibition of human immunodeficiency virus type-1 integrase by curcumin
TL;DR: The present data suggest that HIV-1 integrase inhibition may contribute to the antiviral activity of curcumin, and suggest new strategies for antiviral drug development that could be based uponCurcumin as a lead compound for the development of inhibitors of HIV- 1 integrase.
Journal ArticleDOI
Carcinogenicity and mutagenicity of chromium.
Albert Léonard,Robert Lauwerys +1 more
TL;DR: It can be considered as evident, however, that the ultimate mutagen which binds to the genetic material is the triavalent form produced intracellularly from hexavalent chromium, the apparent lack of activity of the trivalent form being due to its poor cellular uptake.
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A comparison of the in vitro genotoxicity of tri- and hexavalent chromium.
Janusz Blasiak,Joanna Kowalik +1 more
TL;DR: The comet assay did not indicate the involvement of oxidative mechanisms in the DNA-damaging activity of trivalent chromium and it is speculated that its binding to cellular ligands may play a role in its genotoxicity.
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