Journal ArticleDOI
Evidence for p15 cleavage site in myc-specific proteins of avian MC29 and OK10 viruses.
TLDR
Cleavage fragments of p110gag‐myc and p58myc showed the presence of a p15 cleavage site within the myc‐specific region, which is missing in deletion mutants of MC29 virus.Abstract:
Myc-related proteins were precipitated from MC29 virus-transformed cells (PR-2) and from OK10 virus-transformed cells (9C) by anti-gag and anti-myc sera. Immunoprecipitates were cleaved with the avian retroviral protease p15 and the cleavage products analyzed in SDS-PAGE. Cleavage fragments of p110gag-myc (product of MC29 virus) and p58myc (product of OK10 virus) showed the presence of a p15 cleavage site within the myc-specific region. The site is missing in deletion mutants of MC29 virus.read more
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Immunofluorescence detection of the vimentin epitope in chromatin structures of cell nuclei and chromosomes.
TL;DR: Investigation of chromatin structures of eukaryotic cell nuclei and chromosomes using increased sensitivity of 5-bromodeoxyuridine-substituted DNA to UV irradiation-induced crosslinking of DNA with proteins in vivo, by which the proteins interacting with chromosomal DNA can be immunovisualized in situ.
References
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Journal ArticleDOI
Identification of nuclear proteins encoded by viral and cellular myc oncogenes.
Kari Alitalo,Kari Alitalo,Gary Ramsay,J. Michael Bishop,Susan Ohlsson Pfeifer,Wendy W. Colby,Arthur D. Levinson +6 more
TL;DR: It is reported here that v-myc and c- myc encode closely related proteins with molecular weights (MWs) of ∼58,000 and the coding domain for the gene is probably intact.
Journal ArticleDOI
Nucleotide sequence to the v-myc oncogene of avian retrovirus MC29
TL;DR: The nucleotide sequence of v-myc is determined in the genome of the MC29 strain of myelocytomatosis virus, which encodes a hydrophilic polypeptide fused to a portion of the polyprotein encoded by the viral structural gene gag, and may account for the DNA-binding properties of the hybrid gag- myc-encoded protein.
Journal ArticleDOI
Synthesis of Avian RNA Tumor Virus Structural Proteins
Journal ArticleDOI
Avian leukemia viruses oncogenes and genome structure
Thomas Graf,Dominique Stehelin +1 more
Journal ArticleDOI
In vitro cleavage of avian retrovirus gag proteins by viral protease p15
TL;DR: Under the conditions used for Pr76 cleavage, p15 does not introduce breaks into mixtures of cellular proteins eluted in parallel to Pr76 from SDS-containing gels, however, it does fragment proteins that contain all or parts of the amino acid sequence of Pr76.