Journal ArticleDOI
Evidence for the presence of viable endothelial cells in cultures derived from dissociated rat brain.
TLDR
The morphology and histochemistry of dissociated newborn rat brain was studied in tissue culture using direct microscopy of developing cells, electron microscopy and the alkaline phosphatase activity to identify the capillary endothelial cells.Abstract:
The morphology and histochemistry of dissociated newborn rat brain was studied in tissue culture. Direct microscopy of developing cells, electron microscopy and the alkaline phosphatase activity were used to identify the capillary endothelial cells.read more
Citations
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Tumor interactions with the vasculature: angiogenesis and tumor metastasis.
Journal ArticleDOI
Permeability Studies on In Vitro Blood–Brain Barrier Models:Physiology, Pathology, and Pharmacology
TL;DR: Though recent advances resulted in development of improved in vitro BBB model systems to investigate disease modeling, drug screening, and testing vectors targeting the brain, there is a need for checking validity of permeability models and cautious interpretation of data.
Journal ArticleDOI
Role of drug efflux transporters in the brain for drug disposition and treatment of brain diseases
TL;DR: Transporter characteristics for drug efflux transport systems identified in the blood-brain barrier and blood-cerebrospinal fluid (CSF) barrier are summarized to summarize strategies for modulating or by-passingDrug efflux transporters at the BBB as novel therapeutic approaches to drug-resistant brain diseases.
Journal Article
In vitro models of the blood-brain barrier.
TL;DR: The in vitro BBB model is characterized by high transendothelial electrical resistance (TEER regularily above 200 Ohm x cm(2), low permeability and expression of several transporters, which has proven that the model is suitable for basic research and for testing the interaction between the BBB and potential drug candidates (toxicity, permeability, interaction with effluxTransporters) as well.
Journal ArticleDOI
Brain microvessel endothelial cells in tissue culture: a model for study of blood-brain barrier permeability.
TL;DR: Endothelial cells were prepared from bovine brain microvessels and grown in tissue culture and contained factor VIII/von Willebrand antigen, the most specific marker available for determination of the endothelial origin of cells in culture, which accounted for formation of the blood‐brain barrier in vivo.
References
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Journal Article
The localization of monoaminergic blood-brain barrier mechanisms.
TL;DR: The results demonstrate the existence of specialized brain barrier mechanisms at the vascular level and exclude dopamine and D-dopa from the blood-brain barrier.
Journal Article
A procedure for the isolation of capillaries from rat brain.
Joó F,Karnushina I +1 more
Journal ArticleDOI
Interactions of neurons and astrocytes during growth and development of human fetal brain in vitro.
Ben H. Choi,Lowell W. Lapham +1 more
TL;DR: The findings suggest that the relationship between neurons and astrocytes is an important aspect of neural ontogenesis and lend further support to the thesis that astocytes play a role in neuronal migration and differentiation.