Expression of cytokine messenger RNA in normal and neoplastic human breast tissue: Identification of interleukin-8 as a potential regulatory factor in breast tumours
TLDR
Analysis of primary epithelial and stromal cultures derived from both types of tissue showed that increased levels of IL‐8, but not IL‐6, were secreted by cells obtained from tumours, suggesting breast tissue of both normal and neoplastic origin expresses a wide range of cytokines.Abstract:
The presence of mRNA transcripts for cytokines in normal and neoplastic human breast tissue has been investigated. Using reverse transcriptase-linked polymerase chain reaction (RT-PCR), we have specifically screened for the following cytokines: interleukin (IL)-1α, IL-1β, IL-2, IL-3, IL-4, IL-5, IL-6, IL-7, IL-8, tumour necrosis factor (TNF)-α, TNF-β and interferon (IFN)-γ. No significant differences in expression of IL-1α, IL-1β, IL-4, IL-6, TNF-α or TNF-β were observed between the 2 groups of tissues. However, there was a significant difference in expression of IL-8 transcripts (p = 0.0017) which was higher in the neoplastic population. Transcripts for IL-2, IL-3, IL-5, IL-7 and IFN-γ were not detected in either group. There was no evidence of associations between cytokine expression and tumour histological grade, patient age or lymph node metastases. Correlating tumour types with specific cytokine transcripts revealed high expression of IL-8, and to a lesser extent, IL-8 and TNF-β irrespective of tumour origin. Analysis of primary epithelial and stromal cultures derived from both types of tissue showed that increased levels of IL-8, but not IL-6, were secreted by cells obtained from tumours. Thus, breast tissue of both normal and neoplastic origin expresses a wide range of cytokines. Increased or aberrant expression of cytokines, in particular IL-8, may be involved in the development/progression of breast cancer. Int. J. Cancer 72:937–941, 1997. © 1997 Wiley-Liss, Inc.read more
Citations
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Interleukin-8 and human cancer biology.
TL;DR: The role and regulation of IL-8 expression in the growth and metastasis of human cancer with a focus on human pancreatic adenocarcinoma will be discussed.
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Interleukin-8 stimulation of osteoclastogenesis and bone resorption is a mechanism for the increased osteolysis of metastatic bone disease.
TL;DR: Results demonstrate a direct effect of IL-8 on osteoclast differentiation and activity and implicate IL- 8 in the osteolysis associated with metastatic breast cancer.
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Interleukin‐8 is associated with proliferation, migration, angiogenesis and chemosensitivity in vitro and in vivo in colon cancer cell line models
Yan Ning,Philipp C. Manegold,Young-Kwon Hong,Wu Zhang,A. Pohl,Georg Lurje,Thomas Winder,Dongyun Yang,Melissa J. LaBonte,Peter M. Wilson,Robert D. Ladner,Heinz-Josef Lenz +11 more
TL;DR: Findings indicate that overexpression of IL‐8 promotes tumor growth, metastasis, chemoresistance and angiogenesis, implyingIL‐8 to be an important therapeutic target in CRC.
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Significance of interleukin-6 (IL-6) in breast cancer (review)
Heike Knüpfer,Rainer Preiss +1 more
TL;DR: The role of IL-6 in in vitro experiments of breast tumor cells, in breast tumor tissues (BTs) and its potential as a prognostic indicator in breast cancer patients is reviewed and data are surprisingly unique showing IL- 6 to be a negative prognosticator in breast tumors patients.
Journal ArticleDOI
The Potential Role of Neutrophils in Promoting the Metastatic Phenotype of Tumors Releasing Interleukin-8
TL;DR: It is proposed that the host’s cellular response to IL-8 released by tumor cells enhances angiogenesis and contributes to tumor growth and progression and the increase in metastatic potential of tumors ectopically releasing IL- 8 is, in part, attributable to their ability to attract neutrophils.
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