Journal ArticleDOI
Expression of hepatitis C virus NS5B protein: Characterization of its RNA polymerase activity and RNA binding
TLDR
Deletion mutant analysis indicated that the N‐terminal region of NS5B protein was critical for the RNA binding, and this data provide not only important clues for understanding the mechanism of HCV replication, but also a new target of antiviral therapy.About:
This article is published in Hepatology.The article was published on 1999-04-01. It has received 118 citations till now. The article focuses on the topics: RNA-dependent RNA polymerase & NS5B.read more
Citations
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Journal ArticleDOI
Hepatitis C Virus-Encoded Enzymatic Activities and Conserved RNA Elements in the 3′ Nontranslated Region Are Essential for Virus Replication In Vivo
TL;DR: Data suggest that these four HCV-encoded enzymatic activities and the conserved 3′ terminal RNA element are essential for productive replication in vivo.
Journal ArticleDOI
Cyclophilin B Is a Functional Regulator of Hepatitis C Virus RNA Polymerase
Koichi Watashi,Naoto Ishii,Makoto Hijikata,Daisuke Inoue,Takayuki Murata,Yusuke Miyanari,Kunitada Shimotohno +6 more
TL;DR: It is demonstrated that a cellular peptidyl-prolyl cis-trans isomerase, cyclophilin B (CyPB), is critical for the efficient replication of the hepatitis C virus (HCV) genome.
Patent
Nucleoside derivatives as inhibitors of rna-dependent rna viral polymerase
Steven S. Carroll,Malcolm MacCoss,B. David Rahway Olsen,Balkrishen Carlsbad Bhat,Neelima Bhat,Phillip Dan Carlsbad Cook,B. Anne Carlsbad Eldrup,P. Thazha Carlsbad Prakash,Marija Prhavc,Song Quanlai +9 more
TL;DR: In this paper, the authors provided nucleoside compounds and certain derivatives thereof which are inhibitors of RNA-dependent RNA viral polymerase and were useful for the treatment of RNA dependent RNA viral infection.
Book ChapterDOI
Novel insights into hepatitis C virus replication and persistence.
TL;DR: This chapter attempts to summarize the current state of knowledge in these most actively worked on fields of HCV research.
Journal ArticleDOI
De Novo Initiation of RNA Synthesis by the RNA-Dependent RNA Polymerase (NS5B) of Hepatitis C Virus
Guangxiang Luo,Robert K. Hamatake,Danielle M. Mathis,Jason Racela,Karen Rigat,Julie A. Lemm,Richard J. Colonno +6 more
TL;DR: It is suggested that HCV NS5B is able to initiate RNA synthesis de novo, and modification of the RNA template by the addition of the chain terminator cordycepin at the 3′ end did not affect synthesis ofThe RNA monomer but eliminated synthesis of the self-priming hairpin dimer RNA.
References
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Journal ArticleDOI
Isolation of a cDNA clone derived from a blood-borne non-A, non-B viral hepatitis genome
TL;DR: A random-primed complementary DNA library was constructed from plasma containing the uncharacterized non-A, non-B hepatitis agent and screened with serum from a patient diagnosed with NANBH, showing consistent with the agent being similar to the togaviridae or flaviviridae.
Journal ArticleDOI
An assay for circulating antibodies to a major etiologic virus of human non-A, non-B hepatitis
George Kuo,Q L Choo,Harvey J. Alter,G.L. Gitnick,Allan G. Redeker,Robert H. Purcell,Tatsuo Miyamura,Jules L. Dienstag,M.J. Alter,Cladd E. Stevens,Gary E. Tegtmeier,Ferruccio Bonino,Massimo Colombo,W.-S. Lee,C. Kuo,K. Berger,Jeffrey R. Shuster,Lacy Rasco Overby,Daniel W. Bradley,Michael Houghton +19 more
TL;DR: Assays of ten blood transfusions in the United States that resulted in chronic NANBH revealed that there was at least one positive blood donor in nine of these cases and that all ten recipients seroconverted during their illnesses.
Journal ArticleDOI
Genetic organization and diversity of the hepatitis C virus.
Q L Choo,K H Richman,Jang Han,Kim Berger,C Lee,Christine Dong,C Gallegos,D Coit,R Medina-Selby,P J Barr +9 more
TL;DR: The nucleotide sequence of the RNA genome of the human hepatitis C virus has been determined and significant genome diversity is apparent within the putative 5' structural gene region of different HCV isolates, suggesting the presence of closely related but distinct viral genotypes.
Journal ArticleDOI
High-resolution epitope mapping of hGH-receptor interactions by alanine-scanning mutagenesis.
TL;DR: The overall folding of these mutant proteins was indistinguishable from that of the wild-type hGH, as determined by strong cross-reactivities with seven different conformationally sensitive monoclonal antibodies.
Journal ArticleDOI
The natural history of community-acquired hepatitis C in the United States.
Miriam J. Alter,Harold S. Margolis,Krzysztof Krawczynski,Franklyn N. Judson,A Mares,W J Alexander,P Y Hu,J K Miller,Michael A. Gerber,R E Sampliner +9 more
TL;DR: Patients with community-acquired hepatitis C have a high rate of chronic hepatitis, and in most patients HCV infection seems to persist for at least several years, even in the absence of active liver disease.