Journal ArticleDOI
Fluorescent Sensors for Zn2+ Based on a Fluorescein Platform: Synthesis, Properties and Intracellular Distribution
Reads0
Chats0
TLDR
The X-ray crystal structure of a 2:1 Zn(2+):Zinpyr-1 complex has been solved, and is the first structurally characterized example of a complex of fluorescein substituted with metal binding ligands, making them well-suited for intracellular applications.Abstract:
Two new fluorescent sensors for Zn2+ that utilize fluorescein as a reporting group, Zinpyr-1 and Zinpyr-2, have been synthesized and characterized. Zinpyr-1 is prepared in one step via a Mannich reaction, and Zinpyr-2 is obtained in a multistep synthesis that utilizes 4‘,5‘-fluorescein dicarboxaldehyde as a key intermediate. Both Zinpyr sensors have excitation and emission wavelengths in the visible range (∼500 nm), dissociation constants (Kd1) for Zn2+ of <1 nM, quantum yields approaching unity (Φ = ∼0.9), and cell permeability, making them well-suited for intracellular applications. A 3- to 5-fold fluorescent enhancement is observed under simulated physiological conditions corresponding to the binding of the Zn2+ cation to the sensor, which inhibits a photoinduced electron transfer (PET) quenching pathway. The X-ray crystal structure of a 2:1 Zn2+:Zinpyr-1 complex has also been solved, and is the first structurally characterized example of a complex of fluorescein substituted with metal binding ligands.read more
Citations
More filters
Journal ArticleDOI
Fluorescent Sensors for Measuring Metal Ions in Living Systems
Journal ArticleDOI
Metals in Neurobiology: Probing Their Chemistry and Biology with Molecular Imaging
TL;DR: The brain is a singular organ of unique biological complexity that serves as the command center for cognitive and motor function and has requirements for the highest concentrations of metal ions in the body and the highest per-weight consumption of body oxygen.
Journal ArticleDOI
Design strategies for water-soluble small molecular chromogenic and fluorogenic probes.
Journal ArticleDOI
A Colorimetric Lead Biosensor Using DNAzyme-Directed Assembly of Gold Nanoparticles
Juewen Liu,Yi Lu +1 more
TL;DR: The concept developed here can be applied to the design of nucleic acid enzyme/nanoparticle sensors for analytes that are subject to in vitro selection, and thus can significantly expand the scope of nanomaterial applications and provide a novel approach to designing simple colorimetric biosensors.
Journal ArticleDOI
Anion recognition and sensing in organic and aqueous media using luminescent and colorimetric sensors
TL;DR: In this article, a review article focuses primarily on the work carried in our laboratories over the last few years using luminescent and colorimetric sensors, where the anion recognition occurs through hydrogen bonding in organic or aqueous solvents.
References
More filters
Book
Principles of fluorescence spectroscopy
TL;DR: This book describes the fundamental aspects of fluorescence, the biochemical applications of this methodology, and the instrumentation used in fluorescence spectroscopy.
Journal ArticleDOI
Signaling Recognition Events with Fluorescent Sensors and Switches.
A. Prasanna de Silva,H. Q. Nimal Gunaratne,Thorfinnur Gunnlaugsson,Allen J. M. Huxley,Colin P. McCoy,Jude T. Rademacher,Terence E. Rice +6 more
Journal ArticleDOI
The biochemical basis of zinc physiology
TL;DR: Majors topics addressed in this review on zinc physiology are chemistry and biochemistry; interface of biochemistry and physiology of zinc; physiology and cell and molecular biology; and pathology.
Journal ArticleDOI
Cloning and functional characterization of a mammalian zinc transporter that confers resistance to zinc.
TL;DR: It is proposed that ZnT‐1 transports zinc out of cells and that its absence accounts for the increased sensitivity of mutant cells to zinc toxicity.
Related Papers (5)
Fluorescent detection of zinc in biological systems: recent development on the design of chemosensors and biosensors
Pengju Jiang,Zijian Guo +1 more