Journal ArticleDOI
Gaps in DNA synthesized by ultraviolet light-irradiated WI38 human cells.
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TLDR
It is indicated that DNA synthesis after ultraviolet irradiation proceeds in such fashion that gaps are formed along the newly made strand, leaving regions of single strandness in template DNA.About:
This article is published in Biochimica et Biophysica Acta.The article was published on 1976-04-02. It has received 92 citations till now. The article focuses on the topics: DNA replication & DNA synthesis.read more
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Book ChapterDOI
The mechanism of action of antitumor platinum compounds.
J.J. Roberts,Andrew J. Thomson +1 more
TL;DR: The structure–activity relationships discussed in this chapter indicates that platinum complexes superior to those initially described will become available for clinical use.
Journal ArticleDOI
In vivo formation of single-strand breaks in DNA by hydrogen peroxide is mediated by the Haber-Weiss reaction
TL;DR: The chelator therefore removes iron from the chromatin, and although the Fe-EDTA complex formed is capable of reacting with H2O2, the OH radical generated under these conditions is not close enough to hit DNA.
Journal ArticleDOI
Replication fork reactivation downstream of a blocked nascent leading strand.
TL;DR: It is shown that replication can be restarted and leading-strand synthesis re-initiated downstream of an unrepaired block to leading-Strand progression, even when the 3′-OH of the nascent leading strand is unavailable.
Journal ArticleDOI
PrimPol, an Archaic Primase/Polymerase Operating in Human Cells
Sara García-Gómez,Aurelio Reyes,María I. Martínez-Jiménez,E. Sandra Chocron,Silvana Mouron,Gloria Terrados,Christopher A. Powell,Eduardo Salido,Juan Méndez,Ian J. Holt,Luis Blanco +10 more
TL;DR: A second primase in human cells, PrimPol, is described, which has the ability to start DNA chains with deoxynucleotides unlike regular primases, which use exclusively ribonucleotide, which is a DNA polymerase tailored to bypass the most common oxidative lesions in DNA.
Journal ArticleDOI
Replisome assembly and the direct restart of stalled replication forks.
TL;DR: Some recent biochemical observations support models of direct fork repair in which the removal of the blocking template lesion is not always required for replication restart.
References
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Journal ArticleDOI
The serial cultivation of human diploid cell strains.
Leonard Hayflick,P.S. Moorhead +1 more
TL;DR: A consideration of the cause of the eventual degeneration of these strains leads to the hypothesis that non-cumulative external factors are excluded and that the phenomenon is attributable to intrinsic factors which are expressed as senescence at the cellular level.
Journal ArticleDOI
Sedimentation studies of the size and shape of dna.
TL;DR: Analytical zone sedimentation ( Vinograd, Bruner, Kent & Weigle, 1963) is shown to be a sensitive and reliable method for detecting differences in conformation and for determining the molecular weight of homogeneous phage DNA's.
Journal ArticleDOI
Discontinuities in the DNA synthesized in an Excision-defective strain of Escherichia coli following ultraviolet irradiation
W D Rupp,Paul Howard-Flanders +1 more
TL;DR: The amount of tritiated thymidine incorporated into the DNA of irradiated cells indicates that pyrimidine dimers in the DNA inhibit DNA synthesis but are not permanent blocks, and that the daughter-strand DNA synthesized after ultraviolet-irradiation contains gaps, or alkalilabile bonds.
Journal ArticleDOI
Sedimentation rate as a measure of molecular weight of DNA
TL;DR: The results show that high molecular weight DNA's sediment artificially fast in the optical centrifuge, owing to a hitherto unknown effect of molecular interactions.
Journal ArticleDOI
Exchanges between DNA strands in ultraviolet-irradiated Escherichia coli.
TL;DR: Ultraviolet induced pyrimidine dimers remain in the DNA of excision-defective mutants of Escherichia coli for at least several hours after irradiation, indicating the presence of high molecular weight templates virtually free from damaged bases.
Related Papers (5)
Discontinuities in the DNA synthesized in an Excision-defective strain of Escherichia coli following ultraviolet irradiation
W D Rupp,Paul Howard-Flanders +1 more