Journal ArticleDOI
Gene transfer to cereals: an assessment
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This paper reviews the various approaches that have been taken and assesses which of them may provide routine transformation methods for cereals and what biological peculiarities of cereals may hold the key to future success.About:
This article is published in Trends in Biotechnology.The article was published on 1989-10-01. It has received 127 citations till now.read more
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Efficient transformation of rice (Oryza sativa L.) mediated by Agrobacterium and sequence analysis of the boundaries of the T-DNA.
TL;DR: A large number of morphologically normal, fertile, transgenic rice plants were obtained by co-cultivation of rice tissues with Agrobacterium tumefaciens, and sequence analysis revealed that the boundaries of the T-DNA in transgenic Rice plants were essentially identical to those intransgenic dicotyledons.
Journal ArticleDOI
Mesoporous silica nanoparticles deliver DNA and chemicals into plants
TL;DR: A honeycomb mesoporous silica nanoparticle system with 3-nm pores that can transport DNA and chemicals into isolated plant cells and intact leaves and capped the ends with gold nanoparticles to keep the molecules from leaching out is shown.
Journal ArticleDOI
Brachypodium distachyon. A new model system for functional genomics in grasses.
John Draper,Luis A. J. Mur,Glyn Jenkins,Gadab C. Ghosh-Biswas,Pauline Bablak,Robert Hasterok,Andrew P. M. Routledge +6 more
TL;DR: A new model for grass functional genomics is described based on Brachypodium distachyon, which in the evolution of the Pooideae diverged just prior to the clade of "core pooid" genera that contain the majority of important temperate cereals and forage grasses.
Book
Advances in cowpea research
TL;DR: Advances in cowpea research, Advances in animal research, advances in cow pea research as mentioned in this paper, advances in cattle research, and advances in animal agriculture, using cowpeas.
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Transient Gene Expression in Intact and Organized Rice Tissues.
TL;DR: A procedure to electroporate DNA into intact and organized leaf structures of rice and proved that it was equally applicable both to other monocotyledons, including wheat, maize, and barley, and to other explants, such as etiolated and green sheath and lamina tissues from rice.
References
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Journal ArticleDOI
High-velocity microprojectiles for delivering nucleic acids into living cells
TL;DR: In this article, small tungsten particles (microprojectiles) are used to carry RNA or DNA into epidermal tissue of onion and these molecules were subsequently expressed genetically.
High-velocity microprojectiles for delivering nucleic acids into living cells. 1987.
TL;DR: It is reported here a novel phenomenon, namely that nucleic acids can be delivered into plant cells using high-velocity microprojec-tiles, capable of circumventing the host-range restrictions of Agrobacterium tumefaciens1, and the regeneration problems of protoplast transformation2–5.
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Stable transformation of maize after gene transfer by electroporation
TL;DR: It is reported that electroporation-mediated DNA transfer of a chimaeric gene encoding neomycin phosphotransferase results in stably transformed maize cells that are resistant to kanamycin.
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Fertile transgenic rice plants regenerated from transformed protoplasts
TL;DR: The production of fertile transgenic rice plants obtained by introducing the bacterial hph gene, encoding hygromycin B resistance12 (Hmr), into protoplasts of Oryza sativa (L.) by electroporation is reported.
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Genetically transformed maize plants from protoplasts
TL;DR: Genetically transformed maize plants were obtained from protoplasts treated with recombinant DNA and regenerated from transformed cell lines and grown to maturity, indicating the presence of the NPT II gene.