Journal ArticleDOI
High-velocity microprojectiles for delivering nucleic acids into living cells
TLDR
In this article, small tungsten particles (microprojectiles) are used to carry RNA or DNA into epidermal tissue of onion and these molecules were subsequently expressed genetically.Abstract:
We report here a novel phenomenon, namely that nucleic acids can be delivered into plant cells using high-velocity microprojec-tiles. This research was conducted in the hope of circumventing some of the inherent limitations of existing methods for delivering DNA into plant cells1–6. After being accelerated, small tungsten particles (microprojectiles) pierce cell walls and membranes and enter intact plant cells without killing them. Microprojectiles were used to carry RNA or DNA into epidermal tissue of onion and these molecules were subsequently expressed genetically. This approach can therefore be used to study the transient expression of foreign genes in an intact tissue. It remains to be shown that smaller cell types, as are found in regenerable plant tissues, can be stably transformed by this method. If this proves possible, it would appear to provide a broadly applicable transformation mechanism capable of circumventing the host-range restrictions of Agrobacterium tumefaciens1, and the regeneration problems of protoplast transformation2–5.read more
Citations
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Journal ArticleDOI
Nonviral Vectors for Gene Delivery
TL;DR: Two nonviral gene delivery systems using either biodegradable poly(D,Llactide-co-glycolide) (PLG) nanoparticles or cell penetrating peptide (CPP) complexes have been designed and studied using A549 human lung epithelial cells.
Journal ArticleDOI
Biogenesis of Mitochondria
TL;DR: The MITOCHONDRIAL GENETIC SYSTEM, a chronology of key events and events leading to and after the invention of the mitochondria, is described.
Journal ArticleDOI
Chloroplast transformation in Chlamydomonas with high velocity microprojectiles.
John E. Boynton,Nicholas W. Gillham,Elizabeth H. Harris,Jonathan P. Hosler,Anita Johnson,AR Jones,Barbara L. Randolph-Anderson,Dominique Robertson,TM Klein,KB Shark +9 more
TL;DR: Bombardment of three mutants of the chloroplast atpB gene of Chlamydomonas reinhardtii with high-velocity tungsten microprojectiles that were coated with cloned chloropleft DNA carrying the wild-type gene permanently restored the photosynthetic capacity of the algae.
Journal ArticleDOI
Creation of low-copy integrated transgenic lines in Caenorhabditis elegans.
TL;DR: Using microparticle bombardment, it is found that it is possible to create integrated transgenic lines that reproducibly express GFP reporter constructs without the variations in expression level and pattern frequently exhibited by extrachromosomal array lines.
Journal ArticleDOI
Signal transduction through prion protein.
Sophie Mouillet-Richard,Myriam Ermonval,C Chebassier,Jean-Louis Laplanche,Sylvain Lehmann,Jean-Marie Launay,Odile Kellermann +6 more
TL;DR: The murine 1C11 neuronal differentiation model was used to search for PrPc-dependent signal transduction through antibody-mediated cross-linking and a caveolin-1-dependent coupling of PrPC to the tyrosine kinase Fyn was observed.
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