Genes encoding 4-Cys antimicrobial peptides in wheat Triticum kiharae Dorof. et Migush.: multimodular structural organization, instraspecific variability, distribution and role in defence
L. L. Utkina,Yaroslav A. Andreev,Eugene A. Rogozhin,Tatyana V. Korostyleva,Anna A. Slavokhotova,Peter B. Oparin,Alexander A. Vassilevski,Eugene V. Grishin,Tsezi A. Egorov,Tatyana I. Odintsova +9 more
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TLDR
Genes encoding Tk‐AMP‐X precursors have no introns in the protein‐coding regions and are upregulated by fungal pathogens and abiotic stress, providing conclusive evidence for their role in stress response.Abstract:
A novel family of antifungal peptides was discovered in the wheat Triticum kiharae Dorof. et Migusch. Two members of the family, designated Tk-AMP-X1 and Tk-AMP-X2, were completely sequenced and shown to belong to the α-hairpinin structural family of plant peptides with a characteristic C1XXXC2-X(n)-C3XXXC4 motif. The peptides inhibit the spore germination of several fungal pathogens in vitro. cDNA and gene cloning disclosed unique structure of genes encoding Tk-AMP-X peptides. They code for precursor proteins of unusual multimodular structure, consisting of a signal peptide, several α-hairpinin (4-Cys) peptide domains with a characteristic cysteine pattern separated by linkers and a C-terminal prodomain. Three types of precursor proteins, with five, six or seven 4-Cys peptide modules, were found in wheat. Among the predicted family members, several peptides previously isolated from T. kiharae seeds were identified. Genes encoding Tk-AMP-X precursors have no introns in the protein-coding regions and are upregulated by fungal pathogens and abiotic stress, providing conclusive evidence for their role in stress response. A combined PCR-based and bioinformatics approach was used to search for related genes in the plant kingdom. Homologous genes differing in the number of peptide modules were discovered in phylogenetically-related Triticum and Aegilops species, including polyploid wheat genome donors. Association of the Tk-AMP-X genes with A, B/G or D genomes of hexaploid wheat was demonstrated. Furthermore, Tk-AMP-X-related sequences were shown to be widespread in the Poaceae family among economically important crops, such as barley, rice and maize.
Database
Nucleotide sequence data have been deposited in the EMBL database under accession numbers: HF562347, HF562348, HF562349, HF562350, HF562351, HF562352, HF562353, HF562354, HF562355, HF562356, HF562357, HF562358, HF562359, HF562360, HF562361, HF562362, HF562363 and HF562364.read more
Citations
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Journal ArticleDOI
Antimicrobial Peptides from Plants
TL;DR: The ability of plant AMPs to tolerate hypervariable sequences using a conserved scaffold provides diversity to recognize different targets by varying the sequence of the non-cysteine residues, which bode well for developing plant AMP as potential therapeutics and for protection of crops through transgenic methods.
Journal ArticleDOI
The Plant Peptidome: An Expanding Repertoire of Structural Features and Biological Functions
Patrizia Tavormina,Patrizia Tavormina,Barbara De Coninck,Barbara De Coninck,Natalia Nikonorova,Ive De Smet,Bruno P. A. Cammue,Bruno P. A. Cammue +7 more
TL;DR: A novel, unifying peptide classification system is proposed to emphasize the enormous diversity in peptide synthesis and consequent complexity of the still expanding knowledge on the plant peptidome.
Journal ArticleDOI
The use of versatile plant antimicrobial peptides in agribusiness and human health.
Elizabete de Souza Cândido,Marlon H. Cardoso,Daniel Amaro Sousa,Juliane F.C. Viana,Nelson G. Oliveira-Júnior,Vívian de Jesus Miranda,Octavio L. Franco,Octavio L. Franco +7 more
TL;DR: This review will focus on the relevance of the structural-function relations of AMPs derived from plants and their proper use in applications for human health and agribusiness.
Journal Article
Multiple functional proteins are produced by cleaving asn-gln bonds of a single precursor by vacuolar processing enzyme
TL;DR: The results suggested that VPE was responsible for cleaving Asn-Gln bonds of a single precursor, PV100, to produce multiple seed proteins, and it is likely that the Asn -Gln stretches not only provide cleavage sites for VPE but also produce aminopeptidase-resistant proteins.
Journal ArticleDOI
The role of antimicrobial peptides in plant immunity
Marcelo Lattarulo Campos,Marcelo Lattarulo Campos,Camila Maurmann de Souza,Kamila Botelho Sampaio de Oliveira,Simoni Campos Dias,Simoni Campos Dias,Octavio L. Franco,Octavio L. Franco +7 more
TL;DR: It is demonstrated that the capacity of plant AMPs to act against a large spectrum of enemies relies on their diverse mechanism of action and remarkable structural stability.
References
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Journal ArticleDOI
Peptide Antimicrobial Agents
TL;DR: The structural requirements of peptides for antiviral and antibacterial activities are evaluated in light of the diverse set of primary and secondary structures described for host defense peptides.
Journal ArticleDOI
Estimation of globular protein secondary structure from circular dichroism.
S W Provencher,J Glöckner +1 more
TL;DR: In this article, a linear combination of the CD spectra (from 190 to 240 nm) of 16 proteins whose secondary structures are known from X-ray crystallography was used to characterize helix, beta sheet, beta turn, and remainder.
Journal ArticleDOI
Genome evolution in polyploids
TL;DR: Processes and mechanisms of gene and genome evolution in polyploids are reviewed, including the role of transposable elements in structural and regulatory gene evolution; processes and significance of epigenetic silencing; underlying controls of chromosome pairing and mechanisms and functional significance of rapid genome changes are reviewed.
Journal ArticleDOI
MEROPS: the database of proteolytic enzymes, their substrates and inhibitors
TL;DR: The MEROPS database has been expanded to include proteolytic enzymes other than peptidases, and the inclusion of small-molecule inhibitors in the tables of peptidase–inhibitor interactions is included.
Journal ArticleDOI
Antimicrobial Peptides from Plants
Willem F. Broekaert,Bruno P. A. Cammue,M. F. C. De Bolle,Karin Thevissen,G.W. de Samblanx,R. W. Osborn +5 more
TL;DR: All antimicrobial peptides studied thus far appear to exert their antimicrobial effect at the level of the plasma membrane of the target microorganism, but the different peptide types are likely to act via different mechanisms.