Journal ArticleDOI
Genetic and morphological approaches distinguish the three sibling species of the Anisakis simplex species complex, with a species designation as Anisakis berlandi n. sp. for A. simplex sp. C (Nematoda: Anisakidae).
Simonetta Mattiucci,Paolo Cipriani,Stephen C. Webb,Michela Paoletti,Federica Marcer,Bruno Bellisario,David I. Gibson,Giuseppe Nascetti +7 more
TLDR
The morphological and morphometric analyses revealed the presence of morphological features that differed among the 3 biological species, and the tree topologies support the finding that A. simplex (s.s.), A. pegreffii, and A. berlandi n.Abstract:
Numerous specimens of the 3 sibling species of the Anisakis simplex species complex (A. pegreffii, A. simplex (senso stricto)), and A. simplex sp. C) recovered from cetacean species stranded within the known geographical ranges of these nematodes were studied morphologically and genetically. The genetic characterization was performed on diagnostic allozymes and sequences analysis of nuclear (internal transcribed spacer [ITS] of ribosomal [r]DNA) and mitochondrial (mitochondrial [mt]DNA cox2 and rrnS) genes. These markers showed (1) the occurrence of sympatry of the 2 sibling species A. pegreffii and A. simplex sp. C in the same individual host, the pilot whale, Globicephala melas Traill, from New Zealand waters; (2) the identification of specimens of A. pegreffii in the striped dolphin, Stenella coeruleoalba (Meyen), from the Mediterranean Sea; and (3) the presence of A. simplex (s.s.) in the pilot whale and the minke whale, Balaenoptera acutorostrata Lacepede, from the northeastern Atlantic wa...read more
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Large-scale genetic investigation of nematode diversity and their phylogenetic patterns in New Zealand's marine animals
TL;DR: In this article , the authors presented the results of an extensive biodiversity survey and phylogenetic analyses of parasitic nematodes infecting New Zealand's marine animals and presented phylogenies of the dominant clades to illustrate their genetic diversity in New Zealand.
Journal ArticleDOI
Multigene phylogenetic analysis reveals non-monophyly of Anisakis s.l. and Pseudoterranova (Nematoda: Anisakidae).
Tsuyoshi Takano,Naoyasu Sata +1 more
TL;DR: In this article , the authors collected an anisakid third-stage larva and identified it morphologically and molecularly as Pseudoterranova ceticola, and reconstructed the phylogenetic relationship between the two species based on sequences of three mitochondrial (cox1, cox2, and 12S rRNA) and two nuclear (ITS and 28 S rRNA).
Journal ArticleDOI
Quantitative Proteomics Comparison of Total Expressed Proteomes of Anisakis simplex Sensu Stricto, A. pegreffii, and Their Hybrid Genotype.
Susana C. Arcos,Lee Robertson,Sergio Ciordia,Isabel Sánchez-Alonso,Mercedes Careche,Noelia Carballeda-Sanguiao,Miguel González-Muñoz,Alfonso Navas +7 more
TL;DR: The proposed methodology (proteomics and statistical) solidly characterize a set of proteins that are susceptible to take advantage of the new targeted proteomics, and confirms the validity of the method.
Journal ArticleDOI
Molecular Identification of Parasites Isolated from the Stomach of Patients with Anisakis Food Poisoning in Toyama Prefecture, Japan, in 2018
Masanori Watahiki,Kaoru Uchida,Jun-ichi Kanatani,Tomoko Kato,Keiko Kimata,Junko Isobe,Kazunori Oishi,Kana Tozaki,Kenji Sekiguchi,Yasushi Horita,Yasuyuki Morishima,Hiromu Sugiyama +11 more
Journal ArticleDOI
Characterization of Pseudoterranova ceticola (Nematoda: Anisakidae) larvae from meso/bathypelagic fishes off Macaronesia (NW Africa waters)
Miguel Bao,Kaja M. Olsen,Arne Levsen,Paolo Cipriani,Lucilla Giulietti,Julia E. Storesund,Eva García-Seoane,Egil Karlsbakk +7 more
TL;DR: In this paper , the authors described Pseudoterranova ceticola L3 from meso/bathypelagic fishes off Macaronesia, which was morphologically and genetically studied by light microscopy and sequencing of the mtDNA cox2 and entire ITS rDNA genes.
References
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