Journal ArticleDOI
Genetic and morphological approaches distinguish the three sibling species of the Anisakis simplex species complex, with a species designation as Anisakis berlandi n. sp. for A. simplex sp. C (Nematoda: Anisakidae).
Simonetta Mattiucci,Paolo Cipriani,Stephen C. Webb,Michela Paoletti,Federica Marcer,Bruno Bellisario,David I. Gibson,Giuseppe Nascetti +7 more
TLDR
The morphological and morphometric analyses revealed the presence of morphological features that differed among the 3 biological species, and the tree topologies support the finding that A. simplex (s.s.), A. pegreffii, and A. berlandi n.Abstract:
Numerous specimens of the 3 sibling species of the Anisakis simplex species complex (A. pegreffii, A. simplex (senso stricto)), and A. simplex sp. C) recovered from cetacean species stranded within the known geographical ranges of these nematodes were studied morphologically and genetically. The genetic characterization was performed on diagnostic allozymes and sequences analysis of nuclear (internal transcribed spacer [ITS] of ribosomal [r]DNA) and mitochondrial (mitochondrial [mt]DNA cox2 and rrnS) genes. These markers showed (1) the occurrence of sympatry of the 2 sibling species A. pegreffii and A. simplex sp. C in the same individual host, the pilot whale, Globicephala melas Traill, from New Zealand waters; (2) the identification of specimens of A. pegreffii in the striped dolphin, Stenella coeruleoalba (Meyen), from the Mediterranean Sea; and (3) the presence of A. simplex (s.s.) in the pilot whale and the minke whale, Balaenoptera acutorostrata Lacepede, from the northeastern Atlantic wa...read more
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Morphological and genetic identification of Anisakis paggiae (Nematoda: Anisakidae) in dwarf sperm whale Kogia sima from Brazilian waters
Maria Isabel N. Di Azevedo,Marcelo Knoff,Vítor Carvalho,Wildon N Mello,Eduardo José Lopes Torres,Delir Corrêa Gomes,Alena Mayo Iñiguez +6 more
TL;DR: Integrative taxonomic analysis allowed the identification of A. paggiae for the first time in Brazilian waters, providing new data about their geographical distribution and presenting the first SEM images of this species.
Journal ArticleDOI
Potential risk analysis of human anisakiasis through the consumption of mackerel, Scomber scombrus, sold at Spanish supermarkets
Elena Madrid,Florimar Gil,Florimar Gil,María José López García,Ángela L. Debenedetti,María Trelis,Màrius V. Fuentes +6 more
TL;DR: Aside from the recommendation of freezing fish at −20 °C for at least 24 h in case the fish is consumed raw or poorly cooked, the aforementioned factors should be considered by the consumer when purchasing this fish species to avoid any potential anisakiasis risk.
Journal ArticleDOI
Geographic and host size variations as indicators of Anisakis pegreffii infection in European pilchard (Sardina pilchardus) from the Mediterranean Sea: Food safety implications.
Ivana Bušelić,Antonela Botić,Jerko Hrabar,Nika Stagličić,Paolo Cipriani,Simonetta Mattiucci,Ivona Mladineo +6 more
TL;DR: The study demonstrates that the presence of A. pegreffii in European pilchards from the Mediterranean Sea is highly influenced by both geographic and host size variation, which implies that, before future risk management measures are developed, these variables should be assessed in order to minimize public health concerns.
Journal ArticleDOI
A novel nuclear marker and development of an ARMS-PCR assay targeting the metallopeptidase 10 (nas 10) locus to identify the species of the Anisakis simplex (s. l.) complex (Nematoda, Anisakidae).
TL;DR: The novel nuclear diagnostic marker will be useful in future studies on a multi-locus genotyping approach and also to study possible hybridization and/or introgression events occurring between the three species in sympatric areas.
Journal ArticleDOI
Checklist of marine mammal parasites in New Zealand and Australian waters.
TL;DR: A first host–parasite checklist encompassing all available data on parasite prevalence, life cycles and taxonomic relationships in the Australasian region is justified and serves both as a guide for what information is lacking, as well as a practical resource for scientists working on the ecology and conservation of marine mammals.
References
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Principal Component Analysis
TL;DR: In this article, the authors present a graphical representation of data using Principal Component Analysis (PCA) for time series and other non-independent data, as well as a generalization and adaptation of principal component analysis.