Journal ArticleDOI
Genetic and morphological approaches distinguish the three sibling species of the Anisakis simplex species complex, with a species designation as Anisakis berlandi n. sp. for A. simplex sp. C (Nematoda: Anisakidae).
Simonetta Mattiucci,Paolo Cipriani,Stephen C. Webb,Michela Paoletti,Federica Marcer,Bruno Bellisario,David I. Gibson,Giuseppe Nascetti +7 more
TLDR
The morphological and morphometric analyses revealed the presence of morphological features that differed among the 3 biological species, and the tree topologies support the finding that A. simplex (s.s.), A. pegreffii, and A. berlandi n.Abstract:
Numerous specimens of the 3 sibling species of the Anisakis simplex species complex (A. pegreffii, A. simplex (senso stricto)), and A. simplex sp. C) recovered from cetacean species stranded within the known geographical ranges of these nematodes were studied morphologically and genetically. The genetic characterization was performed on diagnostic allozymes and sequences analysis of nuclear (internal transcribed spacer [ITS] of ribosomal [r]DNA) and mitochondrial (mitochondrial [mt]DNA cox2 and rrnS) genes. These markers showed (1) the occurrence of sympatry of the 2 sibling species A. pegreffii and A. simplex sp. C in the same individual host, the pilot whale, Globicephala melas Traill, from New Zealand waters; (2) the identification of specimens of A. pegreffii in the striped dolphin, Stenella coeruleoalba (Meyen), from the Mediterranean Sea; and (3) the presence of A. simplex (s.s.) in the pilot whale and the minke whale, Balaenoptera acutorostrata Lacepede, from the northeastern Atlantic wa...read more
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Novel polymorphic microsatellite loci in Anisakis pegreffii and A. simplex (s. s.) (Nematoda: Anisakidae): implications for species recognition and population genetic analysis
Simonetta Mattiucci,Eleonora Bello,Michela Paoletti,Steve C. Webb,Juan T. Timi,Arne Levsen,Paolo Cipriani,Giuseppe Nascetti +7 more
TL;DR: A panel of seven microsatellite loci was developed and validated on a total of N = 943 specimens of A. pegreffii and A. simplex and discovered two sex-linked loci in both Anisakis species and estimate levels of genetic differentiation at both the inter- and intra-specific level.
Journal ArticleDOI
Differences in Gene Expression Profiles of Seven Target Proteins in Third-Stage Larvae of Anisakis simplex (Sensu Stricto) by Sites of Infection in Blue Whiting (Micromesistius poutassou).
Marialetizia Palomba,Paolo Cipriani,Lucilla Giulietti,Arne Levsen,Giuseppe Nascetti,Simonetta Mattiucci +5 more
TL;DR: Investigating larval gene profiles during the fish infection of the parasitic nematode genus Anisakis found genes encoding some of the target proteins seem to be involved in the host tissue migration and survival of the parasite in the hostile target tissues of the fish host.
Journal ArticleDOI
Species composition and infection dynamics of ascaridoid nematodes in Barents Sea capelin (Mallotus villosus) reflecting trophic position of fish host.
Arne Levsen,Michela Paoletti,Michela Paoletti,Paolo Cipriani,Paolo Cipriani,Giuseppe Nascetti,Simonetta Mattiucci +6 more
TL;DR: It is suggested that the ascaridoid species composition in capelin reflects its trophic position in the Barents Sea ecosystem and there appears to be a link between infection level of the nematode species and the preferred prey organisms of the different developmental phases of capelin.
Journal ArticleDOI
Identification and prevalence of Anisakis pegreffii and A. pegreffii × A. Simplex (s.s.) hybrid genotype larvae in Atlantic horse Mackerel (Trachurus trachurus) from some North African Mediterranean coasts
Alaa Eldin Eissa,Alaa Eldin Eissa,Mohamed L. Showehdi,Mona Ismail,Adnan S. El-Naas,Abdelsalam A. Abu Mhara,Said K. Abolghait +6 more
TL;DR: Results indicated that the highest intensity of infection was recorded in older and female mackerel, which means that the impacts, diversity and epidemiology of A. pegreffii and its hybrid form in North African waters has been provided.
Journal ArticleDOI
Morphological variability, ultrastructure and molecular characterisation of Hysterothylacium reliquens (Norris & Overstreet, 1975) (Nematoda: Raphidascarididae) from the oriental sole Brachirus orientalis (Bloch & Schneider) (Pleuronectiformes: Soleidae)
TL;DR: The results of molecular analysis strongly supported that the broad ranges of morphological variability in the above-mentioned respects in the newly collected specimens of H. reliquens, should be considered as intraspecific variation and the nematode material collected from B. orientalis in the Arabian Gulf represented a single species.
References
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R: A language and environment for statistical computing.
TL;DR: Copyright (©) 1999–2012 R Foundation for Statistical Computing; permission is granted to make and distribute verbatim copies of this manual provided the copyright notice and permission notice are preserved on all copies.
Journal ArticleDOI
Confidence limits on phylogenies: an approach using the bootstrap.
TL;DR: The recently‐developed statistical method known as the “bootstrap” can be used to place confidence intervals on phylogenies and shows significant evidence for a group if it is defined by three or more characters.
Journal ArticleDOI
Bioedit: a user-friendly biological sequence alignment editor and analysis program for windows 95/98/ nt
Book
Principal Component Analysis
TL;DR: In this article, the authors present a graphical representation of data using Principal Component Analysis (PCA) for time series and other non-independent data, as well as a generalization and adaptation of principal component analysis.