Journal ArticleDOI
Genetic and morphological approaches distinguish the three sibling species of the Anisakis simplex species complex, with a species designation as Anisakis berlandi n. sp. for A. simplex sp. C (Nematoda: Anisakidae).
Simonetta Mattiucci,Paolo Cipriani,Stephen C. Webb,Michela Paoletti,Federica Marcer,Bruno Bellisario,David I. Gibson,Giuseppe Nascetti +7 more
TLDR
The morphological and morphometric analyses revealed the presence of morphological features that differed among the 3 biological species, and the tree topologies support the finding that A. simplex (s.s.), A. pegreffii, and A. berlandi n.Abstract:
Numerous specimens of the 3 sibling species of the Anisakis simplex species complex (A. pegreffii, A. simplex (senso stricto)), and A. simplex sp. C) recovered from cetacean species stranded within the known geographical ranges of these nematodes were studied morphologically and genetically. The genetic characterization was performed on diagnostic allozymes and sequences analysis of nuclear (internal transcribed spacer [ITS] of ribosomal [r]DNA) and mitochondrial (mitochondrial [mt]DNA cox2 and rrnS) genes. These markers showed (1) the occurrence of sympatry of the 2 sibling species A. pegreffii and A. simplex sp. C in the same individual host, the pilot whale, Globicephala melas Traill, from New Zealand waters; (2) the identification of specimens of A. pegreffii in the striped dolphin, Stenella coeruleoalba (Meyen), from the Mediterranean Sea; and (3) the presence of A. simplex (s.s.) in the pilot whale and the minke whale, Balaenoptera acutorostrata Lacepede, from the northeastern Atlantic wa...read more
Citations
More filters
Journal ArticleDOI
Proteome profiling of L3 and L4 Anisakis simplex development stages by TMT-based quantitative proteomics.
Robert Stryiński,Jesús Mateos,Santiago Pascual,Ángel F. González,José Manuel Gallardo,Elżbieta Łopieńska-Biernat,Isabel Medina,Mónica Carrera +7 more
TL;DR: Using Tandem Mass Tag (TMT)-based quantitative proteomics, the most comprehensive dataset of proteins of A. simplex for two development stages, L3 and L4, identified to date are analyzed for the first time.
Journal ArticleDOI
Anisakis simplex complex (Nematoda: Anisakidae) in zooplankton communities from temperate NE Atlantic waters
TL;DR: The euphausiid Nyctiphanes couchii and an unidentified mysid have been found, for the first time, with third-stage larvae of the Anisakis simplex complex in the mesozooplanktonic community of the coastal upwelling system in Galicia (NW Spain).
Journal ArticleDOI
Parasites as biological tags to track an ontogenetic shift in the feeding behaviour of Gadus morhua off West and East Greenland
TL;DR: Results indicate an ontogenetic shift in the diet of Atlantic cod Gadus morhua, one of the most important high-level predators of small fish in the North Atlantic, caught during one research vessel cruise from West and East Greenlandic waters.
Journal ArticleDOI
IgE sensitization to Anisakis pegreffii in Italy: Comparison of two methods for the diagnosis of allergic anisakiasis.
Simonetta Mattiucci,Alessandra Colantoni,B Crisafi,F Mori-Ubaldini,L Caponi,P. Fazii,Giuseppe Nascetti,Fabrizio Bruschi +7 more
TL;DR: IgE sensitization to Anisakis pegreffii in Italian subjects suffering from gastro‐allergic anisakiasis (GAA) or showing chronic urticaria after fish consumption, was investigated and 13 proved IgE reactivity, in WB assay, to ESP antigens of A. peGreffii.
Journal ArticleDOI
Occurrence of Anisakis (Nematoda: Anisakidae) larvae in unusual hosts in Southern hemisphere.
TL;DR: Understanding of the extent of infection and the pathogenicity of anisakid nematodes in hosts found in the present study is important in the conservation studies and management plans of these hosts.
References
More filters
Journal Article
R: A language and environment for statistical computing.
TL;DR: Copyright (©) 1999–2012 R Foundation for Statistical Computing; permission is granted to make and distribute verbatim copies of this manual provided the copyright notice and permission notice are preserved on all copies.
Journal ArticleDOI
Confidence limits on phylogenies: an approach using the bootstrap.
TL;DR: The recently‐developed statistical method known as the “bootstrap” can be used to place confidence intervals on phylogenies and shows significant evidence for a group if it is defined by three or more characters.
Journal ArticleDOI
Bioedit: a user-friendly biological sequence alignment editor and analysis program for windows 95/98/ nt
Book
Principal Component Analysis
TL;DR: In this article, the authors present a graphical representation of data using Principal Component Analysis (PCA) for time series and other non-independent data, as well as a generalization and adaptation of principal component analysis.