Genetic Regulation of Ethylene Dosage for Cucumber Fruit Elongation
TLDR
A mechanistic model that governs cucumber (Cucumis sativus) fruit elongation through fine-tuning of ethylene homeostasis is reported, revealing the mechanism by which ethylene dosage is regulated for the control of cell division in developing fruit.Abstract:
Plant organ growth and development are determined by a subtle balance between growth stimulation and inhibition. Fruit size and shape are important quality traits influencing yield and market value; however, the underlying mechanism regulating the balance of fruit growth to achieve final size and shape is not well understood. Here, we report a mechanistic model that governs cucumber (Cucumis sativus) fruit elongation through fine-tuning of ethylene homeostasis. We identified a cucumber mutant that bears short fruits owing to repressed cell division. SF1 (Short Fruit 1) encodes a cucurbit-specific RING-type E3 ligase, and the mutation resulted in its enhanced self-ubiquitination and degradation, but accumulation of ACS2 (1-aminocyclopropane-1-carboxylate synthase 2), a rate-limiting enzyme for ethylene biosynthesis. The overproduction of ethylene contributes to the short-fruit phenotype of sf1. Dysfunction of ACS2 resulted in reduced ethylene production, but still repressed cell division and shorter fruit, suggesting that ethylene is still required for basal fruit elongation. SF1 ubiquitinates and degrades both itself and ACS2 to control ethylene synthesis for dose-dependent effect on cell division and fruit elongation. Our findings reveal the mechanism by which ethylene dosage is regulated for the control of cell division in developing fruit.read more
Citations
More filters
Journal ArticleDOI
Genetic architecture of fruit size and shape variation in cucurbits: a comparative perspective
Yupeng Pan,Yupeng Pan,Yuhui Wang,Cecilia McGregor,Shi Liu,Feishi Luan,Meiling Gao,Yiqun Weng,Yiqun Weng +8 more
TL;DR: The present work illustrates the power of comparative analysis in understanding the genetic architecture of fruit size/shape variation, which may facilitate QTL mapping and cloning for fruit size-related traits in cucurbits.
Journal ArticleDOI
Ethylene signaling in rice and Arabidopsis: New regulators and mechanisms
TL;DR: This review summarizes the latest progress and compares the conserved and divergent aspects of the ethylene signaling pathway between Arabidopsis and rice and discusses how ethylene regulates plant growth, stress responses and agronomic traits.
Journal ArticleDOI
Molecularly tagged genes and quantitative trait loci in cucumber with recommendations for QTL nomenclature
Yuhui Wang,Kailiang Bo,Xingfang Gu,Junsong Pan,Yuhong Li,Jinfeng Chen,Changlong Wen,Zhonghai Ren,Huazhong Ren,Xuehao Chen,Rebecca Grumet,Yiqun Weng,Yiqun Weng +12 more
TL;DR: This is the first attempt to systematically summarize, analyze and inventory cucumber mutants, cloned or mapped genes and QTL, which should be a useful resource for the cucurbit research community.
Journal ArticleDOI
Gene Interactions Regulating Sex Determination in Cucurbits
TL;DR: Recent advances relating to the mechanism of sex determination in cucurbits and the prospects for research in this area are discussed.
Journal ArticleDOI
Resequencing of 297 melon accessions reveals the genomic history of improvement and loci related to fruit traits in melon.
Shi Liu,Peng Gao,Qianglong Zhu,Zicheng Zhu,Hongyu Liu,Xuezheng Wang,Yiqun Weng,Meiling Gao,Feishi Luan +8 more
TL;DR: Novel insights are provided into melon crop evolution, and new tools to advance melon breeding are provided, including CmCLV3 has pleiotropic effects on carpel number and fruit shape.
References
More filters
Journal ArticleDOI
MUSCLE: multiple sequence alignment with high accuracy and high throughput
TL;DR: MUSCLE is a new computer program for creating multiple alignments of protein sequences that includes fast distance estimation using kmer counting, progressive alignment using a new profile function the authors call the log-expectation score, and refinement using tree-dependent restricted partitioning.
Journal ArticleDOI
Transcript-level expression analysis of RNA-seq experiments with HISAT, StringTie and Ballgown
TL;DR: This protocol describes all the steps necessary to process a large set of raw sequencing reads and create lists of gene transcripts, expression levels, and differentially expressed genes and transcripts.
Journal ArticleDOI
Ethylene biosynthesis and its regulation in higher plants
Shang Fa Yang,Neil E. Hoffman +1 more
TL;DR: The pathways of Ethylene Biosynthesis, Regulation in Ripening Fruits and Senescing Flowers and Regulation by Light and Carbon Dioxide are traced.
Journal ArticleDOI
CTR1, a negative regulator of the ethylene response pathway in Arabidopsis, encodes a member of the raf family of protein kinases
TL;DR: A recessive Arabidopsis mutant, ctr1, that constitutively exhibits seedling and adult phenotypes observed in plants treated with the plant hormone ethylene is isolated and the DNA sequences of four mutant alleles were determined.
Journal ArticleDOI
Exploiting the triple response of Arabidopsis to identify ethylene-related mutants.
Plinio Guzmán,Joseph R. Ecker +1 more
TL;DR: Alterations in the response of dark-grown seedlings to ethylene (the "triple response") were used to isolate a collection of ethylene-related mutants in Arabidopsis thaliana and should prove to be useful tools for dissecting the mode of Ethylene action in plants.
Related Papers (5)
A genomic variation map provides insights into the genetic basis of cucumber domestication and diversity
Jianjian Qi,Xin Liu,Di Shen,Han Miao,Bingyan Xie,Xixiang Li,Peng Zeng,Shenhao Wang,Yi Shang,Xingfang Gu,Yongchen Du,Ying Li,Tao Lin,Jinhong Yuan,Xueyong Yang,Jinfeng Chen,Huiming Chen,Xingyao Xiong,Ke Huang,Zhangjun Fei,Linyong Mao,Li Tian,Thomas Städler,Susanne S. Renner,Sophien Kamoun,William J. Lucas,Zhonghua Zhang,Sanwen Huang +27 more