Journal ArticleDOI
Graphene Oxide as a Quencher for Fluorescent Assay of Amino Acids, Peptides, and Proteins
Shanghao Li,Ashish Aphale,Isaac Macwan,Prabir Patra,Walter G. Gonzalez,Jaroslava Miksovska,Roger M. Leblanc +6 more
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TLDR
GO was found to strongly interact with amino acids, peptides, and proteins by fluorescence quenching, indicating GO was a universal quencher for tryptophan or tyrosine related peptides and proteins.Abstract:
Understanding the interaction between graphene oxide (GO) and the biomolecules is fundamentally essential, especially for disease- and drug-related peptides and proteins. In this study, GO was found to strongly interact with amino acids (tryptophan and tyrosine), peptides (Alzheimer’s disease related amyloid beta 1-40 and type 2 diabetes related human islet amyloid polypeptide), and proteins (drug-related bovine and human serum albumin) by fluorescence quenching, indicating GO was a universal quencher for tryptophan or tyrosine related peptides and proteins. The quenching mechanism between GO and tryptophan (Trp) or tyrosine (Tyr) was determined as mainly static quenching, combined with dynamic quenching (Forster resonance energy transfer). Different quenching efficiency between GO and Trp or Tyr at different pHs indicated the importance of electrostatic interaction during quenching. Hydrophobic interaction also participated in quenching, which was proved by the presence of nonionic amphiphilic copolymer ...read more
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Harnessing the chemistry of graphene oxide
TL;DR: An overview of some of the most recent and significant developments in the field of graphene oxide, and an outlook of potential areas where GO, its derivatives, and related materials may be expected to find utility or opportunity for further growth and study.
Journal ArticleDOI
Nanoparticle based fluorescence resonance energy transfer (FRET) for biosensing applications
TL;DR: The rapid pace of development in nanoparticles provides a lot of opportunities to revolutionize FRET techniques and many nanoparticle based FRET assays have also been developed for various biosensing applications with higher sensitivity and better stability compared with traditional organic fluorophore based F RET assays.
Journal ArticleDOI
Nanomaterials for Ultrasensitive Protein Detection
TL;DR: A series of nanomaterials including nanoparticles, nanofibers, nanowires, and nanosheets are reviewed, and their performances in the application for protein detection are evaluated, focusing on approaches that realize ultrasensitive detection.
Journal ArticleDOI
Nontoxic Carbon Dots Potently Inhibit Human Insulin Fibrillation
Shanghao Li,Lingyu Wang,Charles C. Chusuei,Valentina M. Suarez,Patrica L. Blackwelder,Patrica L. Blackwelder,Miodrag Micic,Jhony Orbulescu,Roger M. Leblanc +8 more
TL;DR: In this in vitro study, human insulin was selected as a model to investigate the effect of C-Dots on insulin fibrillation, and water-soluble fluorescent C–Dots were able to efficiently inhibit insulin Fibrillation in a concentration-dependent manner.
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Graphene-based nanosheets for delivery of chemotherapeutics and biological drugs.
TL;DR: The current status of GNS for delivery of anticancer chemotherapeutic agents and biological drugs, with a focus on nucleic acid drugs is covered, including folic acid, transferrins, and others.
References
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TL;DR: Owing to its unusual electronic spectrum, graphene has led to the emergence of a new paradigm of 'relativistic' condensed-matter physics, where quantum relativistic phenomena can now be mimicked and tested in table-top experiments.
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Journal ArticleDOI
Processable aqueous dispersions of graphene nanosheets
TL;DR: It is reported that chemically converted graphene sheets obtained from graphite can readily form stable aqueous colloids through electrostatic stabilization, making it possible to process graphene materials using low-cost solution processing techniques, opening up enormous opportunities to use this unique carbon nanostructure for many technological applications.
Journal ArticleDOI
Atomic structure and chemistry of human serum albumin.
Xiao Min He,Daniel C. Carter +1 more
TL;DR: The three-dimensional structure of human serum albumin has been determined crystallographically to a resolution of 2.8 Å and should provide insight into future pharmacokinetic and genetically engineered therapeutic applications of serumalbumin.
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PEGylated Nanographene Oxide for Delivery of Water-Insoluble Cancer Drugs
TL;DR: The results showed that graphene is a novel class of material promising for biological applications including future in vivo cancer treatment with various aromatic, low-solubility drugs.