Journal ArticleDOI
Hematopoietic reconstitution in a patient with fanconi's anemia by means of umbilical-cord blood from an hla-identical sibling
Eliane Gluckman,Hal E. Broxmeyer,Arleen D. Auerbach,Henry S. Friedman,Gordon W. Douglas,Agnès Devergie,Helene Esperou,Dominique Thierry,Gérard Socié,Pierre Lehn,Scott Cooper,Denis English,Joanne Kurtzberg,Judith Bard,Edward A. Boyse +14 more
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TLDR
It is necessary to select patients suitable for vaginal or laparoscopic mesh placement for Fanconi's anemia preoperatively on the basis of prior history and once they provide informed consent for surgery.Abstract:
The clinical manifestations of Fanconi’s anemia, an autosomal recessive disorder, include progressive pancytopenia, a predisposition to neoplasia, and nonhematopoietic developmental anomalies [1-3]. Hypersensitivity to the clastogenic effect of DNA-cross-linking agents such as diepoxybutane acts as a diagnostic indicator of the genotype of Fanconi’s anemia, both prenatally and postnatally [3-6]. Prenatal HLA typing has made it possible to ascertain whether a fetus is HLA-identical to an affected sibling [7]. We report here on hematopoietic reconstitution in a boy with severe Fanconi’s anemia who received cryo-preserved umbilical-cord blood from a sister shown by prenatal testing to be unaffected by the disorder, to have a normal karyotype, and to be HLA-identical to the patient. We used a pretransplantation conditioning procedure developed specifically for the treatment of such patients [8]; this technique makes use of the hypersensitivity of the abnormal cells to alkylating agents that cross-link DNA [9,10] and to irradiation [11] In this case, the availability of cord blood obviated the need for obtaining bone marrow from the infant sibling. This use of cord blood followed the suggestion of one of us that blood retrieved from umbilical cord at delivery, usually discarded, might restore hematopoiesis – a proposal supported by preparatory studies by some of us [12] and consistent with reports on the presence of hematopoietic stem and multipotential (CFU-GEMM), erythroid (BFU-E), and granulocyte-macrophage (CFU-GM) progenitor cells in human umbilical-cord blood (see the references cited by Broxmeyer et al. [12]).read more
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Journal ArticleDOI
Maternal understanding of commercial cord blood storage for their offspring - a survey among pregnant women in Hong Kong.
Stephen Sik Hung Suen,Terence T. Lao,Oi Ka Chan,Thomas Kam On Kou,Sammy Chung Sum Chan,Jean H. Kim,Tze Kin Lau,Tak Yeung Leung +7 more
TL;DR: The results of this study revealed inadequate knowledge on UCB stem cell banking and its applications among most of the pregnant women and the government and clinicians should combine efforts to provide accurate information on utilization ofUCB stem cells during antenatal care.
Journal ArticleDOI
Stem Cell Biobanks
TL;DR: Stem cells contribute to innate healing and harbor a promising role for regenerative medicine, and constitute fundamental and indivisible modules catalyzed through biobanking activity, generating a return of investment.
Journal ArticleDOI
Cord Blood Banking Standards: Autologous Versus Altruistic
TL;DR: The regulations, standards, and accreditation schemes that are currently available nationally and internationally for public and private CB banking are explored to ensure success and the true realization of the full potential of CB.
Journal ArticleDOI
Relapse of Acute Lymphoblastic Leukemia in Pregnancy: Survival Following Chemoirradiation and Autologous Transfer of Interleukin-2-Activated Stem Cells
Krishnansu S. Tewari,Fabio Cappuccini,Robert B. Rosen,Joseph Rosenthal,Tamerou Asrat,Matthew F. Kohler +5 more
TL;DR: A 20-year-old woman with ALL experienced a relapse during the third trimester of her first pregnancy and was delivered 3 weeks later and the collection of umbilical cord blood from a pregnant woman with leukemia has not been reported previously.
Patent
Enhancement of Natural Killer (NK) Cell Proliferation and Activity
Tony Peled,Gabi M. Frei +1 more
TL;DR: In this article, methods for enhancing propagation and/or functionality of NK cells by treating the cells with an aryl hydrocarbon antagonist in combination with cytokines driving NK cell proliferation are provided.
References
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Journal ArticleDOI
Progress in Clinical and Biological Research
TL;DR: All with an interest in tumours will find something to stimulate them in this book but even these items are to some extent outweighed by the insight given into the thoughts and policies of this fascinating country.
Journal ArticleDOI
Human umbilical cord blood as a potential source of transplantable hematopoietic stem/progenitor cells
Hal E. Broxmeyer,Gordon W. Douglas,Giao Hangoc,Scott Cooper,Judith Bard,Denis English,Margaret Arny,Lewis Thomas,Edward A. Boyse +8 more
TL;DR: It was determined that granulocyte-macrophage, erythroid, and multipotential progenitor cells remained functionally viable in cord blood untreated except for addition of anticoagulant for at least 3 days at 4 degrees C or 25 degrees C (room temperature), though not at 37 degrees C, implying that these cells could be satisfactorily studied and used or cryopreserved for therapy.
Journal ArticleDOI
Susceptibility of Fanconi's anaemia fibroblasts to chromosome damage by carcinogens
TL;DR: Experiments in which viable FA fibroblasts were exposed to a direct-acting mutagen or carcinogen for a period of 6 d, ensuring chronic exposure of the cells during one or more cell cycles, until increased cell density inhibited further cell division.
Journal Article
Familial constitutional panmyelocytopathy, Fanconi's anemia (F.A.). I. Clinical aspects.
Journal ArticleDOI
International Fanconi Anemia Registry: relation of clinical symptoms to diepoxybutane sensitivity.
TL;DR: It is concluded that hypersensitivity to the clastogenic effect of DEB is a useful discriminator for FA and a simplified scoring method for classifying patients on the basis of eight clinical manifestations that are the best predictors for FA is presented.