Journal ArticleDOI
Heme regulates transcription of the CYC1 gene of S. cerevisiae via an upstream activation site
Leonard Guarente,Thomas L. Mason +1 more
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TLDR
expression of the iso-1-cytochrome c gene of Saccharomyces cerevisiae, CYC1, is tightly regulated by levels of intracellular heme, suggesting that in the wildtype, heme controls initiation per se and not translation or mRNA stability.About:
This article is published in Cell.The article was published on 1983-04-01. It has received 577 citations till now. The article focuses on the topics: Upstream activating sequence & Promoter.read more
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Transcriptional Paradigms in Mammalian Mitochondrial Biogenesis and Function
TL;DR: These transcriptional paradigms provide a basic framework for understanding the integration of mitochondrial biogenesis and function with signaling events that dictate cell- and tissue-specific energetic properties.
Journal ArticleDOI
Transcriptional induction of genes encoding endoplasmic reticulum resident proteins requires a transmembrane protein kinase
TL;DR: IRE1 encodes a transmembrane serine/threonine kinase that it is proposed transmits the unfolded protein signal across the ER or inner nuclear membrane, suggesting that the induction of ER resident proteins is coupled to the biogenesis of new ER membrane.
Journal ArticleDOI
Ssn6-Tup1 is a general repressor of transcription in yeast.
TL;DR: It is proposed that Ssn6-Tup1 is a general repressor of transcription in yeast, recruited to target promoters by a variety of sequence-specific DNA-binding proteins.
Journal ArticleDOI
Negative effect of the transcriptional activator GAL4
Grace Gill,Mark Ptashne +1 more
TL;DR: It is suggested that this inhibition, which the authors call squelching, reflects titration of a transcription factor by the activating region of GAL4, and that more efficient activators inhibit more strongly and that inhibition does not depend on the DNA-binding domain.
Journal ArticleDOI
A molecular link between stem cell regulation and floral patterning in Arabidopsis.
Jan U. Lohmann,Ray L. Hong,Ray L. Hong,Martin Hobe,Maximilian A. Busch,François Parcy,Rüdiger Simon,Detlef Weigel,Detlef Weigel +8 more
TL;DR: It is shown that the floral identity protein LEAFY, a transcription factor expressed throughout the flower, cooperates with the homeodomain protein WUSCHEL (WUS) to activate AG in the center of flowers, creating a negative feedback loop that is required for the determinate growth of floral meristems.
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Journal Article
Protein Measurement with the Folin Phenol Reagent
TL;DR: Procedures are described for measuring protein in solution or after precipitation with acids or other agents, and for the determination of as little as 0.2 gamma of protein.
Journal ArticleDOI
Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: procedure and some applications.
TL;DR: A method has been devised for the electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets that results in quantitative transfer of ribosomal proteins from gels containing urea.
Book
Experiments in molecular genetics
TL;DR: Molecular Genetics (Biology): An Overview | Sciencing Experimental in Molecular Genetics Experiments in molecular genetics (1972 edition) | Open ...
Book ChapterDOI
Sequencing end-labeled DNA with base-specific chemical cleavages.
Allan M. Maxam,Walter Gilbert +1 more
TL;DR: The chapter presents techniques for producing discrete DNA fragments, end-labeling DNA, segregating end- labeled fragments, extracting DNA from gels, and the protocols for partially cleaving it at specific bases using the chemical reactions.
Journal ArticleDOI
Hybridization of denatured RNA and small DNA fragments transferred to nitrocellulose.
TL;DR: A simple and rapid method for transferring RNA from agarose gels to nitrocellulose paper for blot hybridization has been developed, allowing removal of the hybridized probes and rehybridization of the RNA blots without loss of sensitivity.