Journal ArticleDOI
High-Capacity, Protein-Binding Membranes Based on Polymer Brushes Grown in Porous Substrates
TLDR
The use of atom transfer radical polymerization to grow poly(2-hydroxyethyl methacrylate) brushes in porous alumina followed by functionalization of the PHEMA with nitrilotriacetate−Cu2+ complexes yields membranes that adsorb proteins via coordination of Cu2+ to histidine residues that have unusually high capacity for rapid protein binding.Abstract:
The use of atom transfer radical polymerization to grow poly(2-hydroxyethyl methacrylate) (PHEMA) brushes in porous alumina followed by functionalization of the PHEMA with nitrilotriacetate−Cu2+ complexes yields membranes that adsorb proteins via coordination of Cu2+ to histidine residues. Adsorption isotherms show that these membranes have binding capacities as high as 0.9 mg of bovine serum albumin (BSA)/cm2 of external membrane surface area (150 mg/cm3 of membrane), and breakthrough curves indicate that saturation of the membranes with BSA or myoglobin occurs in less than 15 min. The efficiency of protein elution with ethylenediaminetetraacetic acid (EDTA) solutions is essentially 100%, and the membranes show no detectable decrease in capacity over nine cycles of binding, elution, and regeneration with Cu2+. The unusually high capacity of these membranes for rapid protein binding makes them attractive for applications such as purification of His-tag proteins.read more
Citations
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Journal ArticleDOI
Polymer Brushes via Surface-Initiated Controlled Radical Polymerization: Synthesis, Characterization, Properties, and Applications
Raphael Barbey,Laurent Lavanant,Dusko Paripovic,Nicolas Schüwer,Caroline Sugnaux,Stefano Tugulu,Harm-Anton Klok +6 more
TL;DR: This data indicates that self-Assembled Monolayers and Walled Carbon Nanotubes with high adhesion to Nitroxide-Mediated Polymerization have potential in the well-Defined Polymer Age.
Journal ArticleDOI
Porous anodic aluminum oxide: anodization and templated synthesis of functional nanostructures.
Woo Lee,Sang-Joon Park +1 more
Journal ArticleDOI
Surface-Initiated Controlled Radical Polymerization: State-of-the-Art, Opportunities, and Challenges in Surface and Interface Engineering with Polymer Brushes
Justin Orazio Zoppe,Nariye Cavusoglu Ataman,Piotr Mocny,Jian Wang,John Moraes,Harm-Anton Klok +5 more
TL;DR: The generation of polymer brushes by surface-initiated controlled radical polymerization (SI-CRP) techniques has become a powerful approach to tailor the chemical and physical properties of interfaces and has given rise to great advances in surface and interface engineering as mentioned in this paper.
Journal ArticleDOI
Membrane-based techniques for the separation and purification of proteins: an overview.
TL;DR: This manuscript provides an overview of recent developments and published literature in membrane technology, focusing on special characteristics of the membranes and membrane-based processes that are now used for the production and purification of proteins.
Journal ArticleDOI
Nanoporous anodic aluminium oxide: Advances in surface engineering and emerging applications
TL;DR: Anodic aluminium oxide (AAO) has been investigated and utilized in numerous products for almost a century as discussed by the authors. But the rapidly increasing interest in nanoscale materials and their outstanding properties has propelled nanoporous AAO to the fore as one of the most popular nanomaterial with applications across a gamut of areas including molecular separation, catalysis, energy generation and storage, electronics and photonics, sensors and biosensors, drug delivery and template synthesis.
References
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Journal ArticleDOI
Nanomaterials: a membrane-based synthetic approach.
TL;DR: A relatively new method for preparing nanomaterials, membrane-based synthesis, is reviewed, which entails synthesis of the desired material within the pores of a nanoporous membrane.
Journal ArticleDOI
Polymers at interfaces : Using atom transfer radical polymerization in the controlled growth of homopolymers and block copolymers from silicon surfaces in the absence of untethered sacrificial initiator
Krzysztof Matyjaszewski,Peter J. Miller,Nisha Shukla,Boonchuan Immaraporn,Andrew Gelman,Barry B. Luokala,Tiberiu Mircea Siclovan,Guido Kickelbick,Thomas Vallant,Helmuth Hoffmann,Tadeusz Pakula +10 more
TL;DR: The atom transfer radical polymerization (ATRP) of styrene and acrylates from silicon wafers modified with an initiator layer composed of 2-bromoisobutyrate fragments is described in this paper.
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A strategy for the generation of surfaces presenting ligands for studies of binding based on an active ester as a common reactive intermediate: a surface plasmon resonance study.
TL;DR: Ten proteins and two low-molecular-weight ligands were immobilized on mixed self-assembled monolayers (SAMs) of alkanethiolates on gold generated from the tri(ethylene glycol)-terminated thiol 1 and these SAMs were resistant to nonspecific adsorption of proteins having a wide range of molecular weights and isoelectric points.
Journal ArticleDOI
Separation of biomolecules using adsorptive membranes
TL;DR: A review of adsorptive separations using affinity, ion exchange and hydrophobic membranes can be found in this paper, where the authors show that these membranes exhibit low backpressure, short residence times and high volumetric throughputs relative to conventional chromatographic packed beds.
Journal ArticleDOI
Functionalization of surfaces by water-accelerated atom-transfer radical polymerization of hydroxyethyl methacrylate and subsequent derivatization
TL;DR: In this article, the synthesis of unusually thick poly(2-hydroxyethyl methacrylate) (PHEMA) films on gold surfaces by surface-initiated atom transfer radical polymerization (ATRP) was reported.