High prevalence of hepatitis B virus genotype C/C1 in the Minangkabau ethnic group in Indonesia
Marlinang Diarta Siburian,Andi Utama,Rama Dhenni,Arnelis,Ismail Fanany,Mariana Db Intan,Tri Shinta Kurniasih,Febi Andriani,Szeifoul Afadlal,Erlys B. Julianto,Widyarman S Rasman,Nasrul Zubir,George Mathew +12 more
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TLDR
HBV/C with subgenotype C1 was the predominant HBV genotype among HBV carriers of Minangkabau ethnicity and the prevalence of pre-S, T1753V and A1762T/G1764A mutations was higher among the MinangKabau compared to IndonesianHBV carriers in general.Abstract:
The Minangkabau is one of the major ethnic groups in Indonesia. Previous studies with a limited number of samples have shown a different prevalence of HBV/C in the Minangkabau compared to the Indonesian population in general. The aim of this study was to assess the HBV genotype distribution pattern and the prevalence of pre-S, T1753V and A1762T/G1764A mutations among the Minangkabau HBV carriers. The samples were collected from Padang, West Sumatera and from western Java. Mixed primers for specific genotypes were used to determine the HBV genotype. Pre-S or S genes were amplified, sequenced and aligned with reference sequences from GenBank to derive a phylogenetic tree for subgenotyping. Pre-S genes were also analyzed for mutations. The basal core promoter (BCP) region was amplified and directly sequenced to analyze T1753V and A1762T/G1764A mutations. The predominant HBV genotype among the Minangkabau HBV carriers (n=117) was C (72.6%) followed by B (24.8%) and co-infection with B and C (2.6%). The prevalence of pre-S mutations, including both the pre-S deletion and pre-S2 start codon mutation, was 41.0%, and the T1753V and A1762T/G1764A mutations were found in 51.9% and 71.2% respectively. HBV/C1 was the predominant HBV subgenotype in the Minangkabau HBV carriers, and was found in 66.2%, followed by B3, B7, C8, B2, B9, C2, and C10 (18.3%, 7.0%, 2.8%, 1.4%, 1.4%, 1.4%, and 1.4% respectively). From samples that were found to be co-infected with HBV B and C, two samples were successfully cloned and subgenotyped, including one with mixed subgenotypes of B3 and C1, and another one with mixed subgenotypes of B7, C1, putative intergenotypic of B/A, and C/A. Furthermore, three samples from donors of non-Minangkabau ethnicity from Padang were found to be infected with an intragenotypic recombination form, including a putative recombinant of B8/B3 and B9/B7. HBV/C with subgenotype C1 was the predominant HBV genotype among HBV carriers of Minangkabau ethnicity. The prevalence of pre-S, A1762T/G1764A, and T1753V mutations was higher among the Minangkabau compared to Indonesian HBV carriers in general.read more
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References
More filters
Journal ArticleDOI
An overview of molecular epidemiology of hepatitis B virus (HBV) in India
TL;DR: The clinical significance of HBV genotypes, core promoter and precore mutations, possible routes of introduction ofHBV genotype C in eastern India, the clinical implications of x gene variability, prevalence of the AFB1 induced p53 gene codon 249 mutation, the transmission potentiality of HBVs among asymptomatic/inactive or occult HBV carriers and the genetic variability of HBv persisting in the PBL was investigated.
Journal ArticleDOI
The natural history of chronic HBV infection and geographical differences
TL;DR: Clinical studies have shown that chronic HBV infection in western countries is associated with a higher incidence of cirrhosis, but lower incidence of hepatocellular carcinoma, than in Asian countries.
Journal ArticleDOI
High Prevalence of Hepatitis B Virus Pre-S Mutant in Countries Where It Is Endemic and Its Relationship with Genotype and Chronicity
Tran Thien Tuan Huy,Tran Thien Tuan Huy,Tran Thien Tuan Huy,Hiroshi Ushijima,Khin Maung Win,Pairoj Luengrojanakul,Pradeep Krishna Shrestha,Zhao-Hua Zhong,Andrei V. Smirnov,Teresa Casanovas Taltavull,Tetsutaro Sata,Kenji Abe +11 more
TL;DR: There was a high prevalence of HBV pre-S mutation in regions of endemic HBV infection in Asia and this mutation appeared to be correlated with hepatocellular carcinoma and HBV genotypes.
Journal ArticleDOI
Novel Subgenotypes of Hepatitis B Virus Genotypes C and D in Papua, Indonesia
Maria Inge Lusida,Victor Eka Nugrahaputra,Soetjipto,Retno Handajani,Motoko Nagano-Fujii,Mikiko Sasayama,Takako Utsumi,Hak Hotta +7 more
TL;DR: Two novel HBV subgenotypes are proposed, HBV/C6 andHBV/D6, the first of which is the most prevalent subgenotype of HBV in Papua, Indonesia, possibly representing a unique antigenic group (provisionally referred to as adrq indeterminate).
Journal ArticleDOI
Population genetic structure of peninsular Malaysia Malay sub-ethnic groups.
Wan Isa Hatin,Ab Rajab Nur-Shafawati,Mohd Khairi Zahri,Shuhua Xu,Li Jin,Soon Guan Tan,Mohammed Rizman-Idid,Bin Alwi Zilfalil +7 more
TL;DR: The phylogeny of populations showed that all of the four Malay sub-ethnic groups are separated into at least three different clusters, and genetic structuring among the Malay populations could possibly be accounted for by their different historical origins.