Journal ArticleDOI
Homology between chitinases that are induced by TMV infection of tobacco
R. A. M. Hooft van Huijsduijnen,S. Kauffmann,Frans Th. Brederode,Ben J. C. Cornelissen,M. Legrand,B. Fritig,John F. Bol +6 more
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TLDR
Hybridization of cDNA probes to genomic blots indicated that the acidic and basic chitinases are each encoded by two to four genes in the amphidiploid genome of Samsun NN tobacco.Abstract:
Recently, four chitinases have been detected in tobacco mosaic virus (TMV) infected tobacco: two acidic chitinases that were identified as pathogenesis-related (PR) proteins P and Q and two basic chitinases (Legrand et al., Proc.Natl. Acad. Sci. USA, in press). Here, it was shown that P and Q are closely serologically related but not related to other known acidic tobacco PR proteins. Antisera to P and Q were used to characterize translation products of TMV-induced mRNAs that were hybrid-selected with cDNA clones described previously (Hooft van Huijsduijnen et al., EMBO J 5: 2057–2061, 1986). In this way cDNA clones corresponding to the acidic and basic chitinases were identified. The partial amino acid sequences of the acidic and basic tobacco chitinases that were represented in the clones, showed an approximately 70% homology to each other and to the sequence of a bean chitinase. Although the acidic and basic chitinases differ in apparent molecular weight, they were found to have homologous C-termini. Hybridization of cDNA probes to genomic blots indicated that the acidic and basic chitinases are each encoded by two to four genes in the amphidiploid genome of Samsun NN tobacco. A similar complexity was found for the genes encoding the tobacco PR protein that is homologous to the sweet-tasting protein thaumatin and to the bifunctional trypsin/α-amylase inhibitor from maize.read more
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Journal ArticleDOI
Plant ‘pathogenesis-related’ proteins and their role in defense against pathogens
Annick Stintzi,Thierry Heitz,Vivek Prasad,S. Wiedemann-Merdinoglu,Serge Kauffmann,Pierrette Geoffroy,Michel Legrand,Bernard Fritig +7 more
TL;DR: Several members of the PR-5 group from tobacco and other plant species were shown to display significant in vitro activity of inhibiting hyphal growth or spore germination of various fungi probably by a membrane permeabilizing mechanism.
Journal ArticleDOI
Pathogenesis-related proteins of plants
TL;DR: Frequently, the hypersensitively reacting host becomes resistant to subsequent pathogenic attack and the correlation of this phenomenon of acquired resistance and the induced protein synthesis suggests a direct involvement of these proteins in the observed replication deficiency of the pathogen.
Patent
Disease-resistant transgenic plants
John A. Ryals,Danny C. Alexander,Robert M. Goodman,Frederick Prof. Meins,George B. Payne,Jeffrey R. Stinson,Jean-Marc Dr. Neuhaus,Moyer Mary B,Eric Russell Ward,Shericca Cherrer Williams +9 more
TL;DR: In this paper, a chimeric DNA construct useful for producing transgenic disease-resistant plants and to genetic engineering of plants to produce the phenotype of disease resistance has been proposed for the purpose of making transgenic plants constitutively express induced levels of plant PRPs or substantially homologous proteins.
Book ChapterDOI
Activation, structure, and organization of genes involved in microbial defense in plants
TL;DR: This chapter discusses the recent progress made in the molecular genetics of activation of plant defenses in response to pathogen attack with primary consideration given to the resistance to fungal and bacterial pathogens by members of the plant family Leguminosae.
Journal ArticleDOI
What's new in chitinase research?
TL;DR: This review article deals with recent developments in molecular and physiological aspects of chitinases from plants, fungi, bacteria, insects and fishes.
References
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Cleavage of Structural Proteins during the Assembly of the Head of Bacteriophage T4
TL;DR: Using an improved method of gel electrophoresis, many hitherto unknown proteins have been found in bacteriophage T4 and some of these have been identified with specific gene products.
Journal Article
Cleavage of structural proteins during the assemble of the head of bacterio-phage T4
TL;DR: Using an improved method of gel electrophoresis, many hitherto unknown proteins have been found in bacteriophage T4 and some of these have been identified with specific gene products as mentioned in this paper.
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Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: procedure and some applications.
TL;DR: A method has been devised for the electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets that results in quantitative transfer of ribosomal proteins from gels containing urea.
Journal ArticleDOI
Labeling deoxyribonucleic acid to high specific activity in vitro by nick translation with DNA polymerase I
TL;DR: Labeled DNAs (and restriction endonuclease fragments derived from them) are useful probes for detecting rare homologous sequences by in situ hybridization and reassociation kinetic analysis.
Journal ArticleDOI
Plant chitinases are potent inhibitors of fungal growth
TL;DR: It is reported here that the main proteinaceous inhibitor of fungal growth in bean leaves is chitinase, an enzyme that can be induced by the plant hormone ethylene, or by pathogen attack.