Journal ArticleDOI
Human urine biomarkers of renal cell carcinoma evaluated by ClinProt.
Niccolò Bosso,Clizia Chinello,Stefano Picozzi,Erica Gianazza,Veronica Mainini,Carmen Galbusera,Francesca Raimondo,Roberto A. Perego,Stefano Casellato,Francesco Rocco,Stefano Ferrero,Silvano Bosari,Paolo Mocarelli,Marzia Galli Kienle,Fulvio Magni +14 more
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TLDR
The efficacy of this cluster of signals to distinguish RCC patients grouped by tumor stage showed a sensibility of 100% for patients at the primary tumor 1 stage.Abstract:
Renal cell carcinoma (RCC) is one of the major causes of cancer death and is radio- and chemoresistant. Urine of 29 healthy subjects and 39 clear cell RCC patients were analyzed using the ClinProt technique to search for possible biomarkers for early RCC diagnosis. A cluster of three signals (marker A= at m/z 1827 ± 8 Da, marker B = 1914 ± 8 Da and marker C = 1968 ± 8 Da) was able to discriminate patients from controls. A receiver operating characteristic curve analysis showed values of area under the curve (AUC) higher than 0.9 for marker A and B, corresponding to a sensitivity of 85-90% and a specificity of 90%, while marker C gave a lower AUC (0.84) corresponding to sensitivity of 70% and specificity of 100%. The combination of three markers lead to an improvement in diagnostic efficacy, with specificity and sensitivity of 100% and 95%, respectively, in the training test and of 100% and of 85% in the test experiment. The efficacy of this cluster of signals to distinguish RCC patients grouped by tumor stage showed a sensibility of 100% for patients at the primary tumor 1 stage. One of the signals present in the cluster was identified as a fragment of Tamm-Horsfall protein.read more
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Journal ArticleDOI
Proteomic studies of urinary biomarkers for prostate, bladder and kidney cancers
TL;DR: The development of robust, easy-to-use clinical tests based on novel biomarkers has the potential to impact upon diagnosis, prognosis and monitoring and could revolutionize the treatment and management of urological cancers.
Journal ArticleDOI
Alterations of the serum peptidome in renal cell carcinoma discriminating benign and malignant kidney tumors.
Erica Gianazza,Clizia Chinello,Veronica Mainini,Marta Cazzaniga,Valeria Squeo,Giancarlo Albo,Stefano Signorini,Salvatore S. Di Pierro,Stefano Ferrero,Simone Nicolardi,Yuri E. M. van der Burgt,André M. Deelder,Fulvio Magni +12 more
TL;DR: The results suggest the possibility to discriminate malignant kidney tumors based on a cluster of serum peptides and label-free approach may represent a valid method to verify results obtained by MALDI-TOF.
Journal ArticleDOI
Review and Comparison of Cancer Biomarker Trends in Urine as a Basis for New Diagnostic Pathways.
TL;DR: This investigation aims to evaluate the possibility to compare the results of studies based on different approaches to be able in the future to identify those compounds responsible for urine odor alteration and to highlight the correlation between urine odor alterations and cancer presence.
Journal ArticleDOI
Proteomic analysis reveals differentially secreted proteins in the urine from patients with clear cell renal cell carcinoma
Vanessa Sandim,Denise de Abreu Pereira,Dário Eluan Kalume,Dário Eluan Kalume,Ana Lucia Oliveira-Carvalho,Antonio Augusto Ornellas,Márcia Regina Soares,Gilda Alves,Russolina B. Zingali +8 more
TL;DR: The strategies used proved to be valuable in identifying proteins that were differentially secreted in urine from patients with RCC, and kininogen-1, apolipoprotein D, fibrinogen, and haptoglobin presented similar quantitative protein profiles according to MS(E) and 2DE approaches.
Journal ArticleDOI
Applications of urinary proteomics in biomarker discovery
Chen Shao,Yan Wang,Youhe Gao +2 more
TL;DR: It is proposed that establishing the natural variation in urinary protein expression among a healthy population can serve as a reference to help identify protein abundance changes that are caused by disease, not by individual variations or physiological changes.
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