Hybrid genotypes in the pathogenic yeast Cryptococcus neoformans
Teun Boekhout,Bart Theelen,Mara R. Diaz,Jack W. Fell,Wim C. J. Hop,Edwin C.A. Abeln,Françoise Dromer,Wieland Meyer +7 more
TLDR
The authors propose the existence of two species, C. neoformans (Sanfelice) Vuillemin and C. bacillisporus Kwon-Chung, which differ in geographical distribution, serotypes and ecological origin, and within each species three AFLP genotypes occur.Abstract:
Amplified fragment length polymorphism (AFLP) genotyping of isolates of the pathogenic fungus Cryptococcus neoformans suggested a considerable genetic divergence between the varieties C. neoformans var. neoformans and C. neoformans var. grubii on the one hand versus C. neoformans var. gattii on the other. This divergence is supported by additional phenotypic, biochemical, clinical and molecular differences. Therefore, the authors propose the existence of two species, C. neoformans (Sanfelice) Vuillemin and C. bacillisporus Kwon-Chung, which differ in geographical distribution, serotypes and ecological origin. Within each species three AFLP genotypes occur, which differ in geographical distribution and serotypes. Differences in ecological origin (AIDS patients, non-AIDS patients, animals or the environment) were found to be statistically not significant. In C. neoformans as well as in C. bacillisporus one of the genotypes represented a hybrid. The occurrence of hybridization has consequences for the reproductive biology of the species, as new genotypes with altered virulence or susceptibility to antifungal drugs may arise through the exchange of genetic material.read more
Citations
More filters
Journal ArticleDOI
Epidemiology of Invasive Mycoses in North America
TL;DR: The epidemiologic profiles of these invasive mycoses in North America are discussed, as well as risk factors for infection, and the pathogens’ antifungal susceptibility.
Journal ArticleDOI
A rare genotype of Cryptococcus gattii caused the cryptococcosis outbreak on Vancouver Island (British Columbia, Canada)
Sarah E. Kidd,Ferry Hagen,R L Tscharke,M Huynh,Karen H Bartlett,Murray Fyfe,Laura MacDougall,Teun Boekhout,Kyung J. Kwon-Chung,Wieland Meyer +9 more
TL;DR: The emergence of this usually tropical pathogen on Vancouver Island highlights the changing distribution of this genotype and emphasizes the importance of an ongoing collaborative effort to monitor the global epidemiology of this yeast.
Journal ArticleDOI
Recognition of seven species in the Cryptococcus gattii/Cryptococcus neoformans species complex.
Ferry Hagen,Kantarawee Khayhan,Bart Theelen,Anna Kolecka,Itzhack Polacheck,Edward Sionov,Edward Sionov,Rama Falk,Rama Falk,Sittiporn Parnmen,H. Thorsten Lumbsch,Teun Boekhout,Teun Boekhout +12 more
TL;DR: MALDI-TOF mass spectrometry readily distinguishes the newly recognized species, which differ in aspects of pathogenicity, prevalence for patient groups, as well as biochemical and physiological aspects, such as susceptibility to antifungals.
Journal ArticleDOI
Molecular Typing of IberoAmerican Cryptococcus neoformans Isolates
TL;DR: To acquire basic knowledge of Cryptococcus neoformans in IberoAmerican countries, 340 clinical, veterinary, and environmental isolates from Argentina, Brazil, Chile, Colombia, Mexico, Peru, Venezuela, Guatemala, and Spain were typed by using M13 polymerase chain reaction-fingerprinting and orotidine monophosphate pyrophosphorylase gene restriction fragment length polymorphsm analysis.
Journal ArticleDOI
Same-sex mating and the origin of the Vancouver Island Cryptococcus gattii outbreak
James A. Fraser,Steven S. Giles,Emily C. Wenink,Scarlett Geunes-Boyer,Jo Rae Wright,Stephanie Diezmann,Andria Allen,Jason E. Stajich,Fred S. Dietrich,John R. Perfect,Joseph Heitman +10 more
TL;DR: It is demonstrated how cryptic same-sex reproduction can enable expansion of a human pathogen to a new geographical niche and contribute to the ongoing production of infectious spores.
References
More filters
Journal ArticleDOI
AFLP: a new technique for DNA fingerprinting.
Pieter Vos,René Cornelis Josephus Hogers,Marjo Bleeker,Martin Reijans,Theo van de Lee,Miranda Hornes,Adrie Friters,Jerina Pot,Johan Paleman,Martin Kuiper,Marc Zabeau +10 more
TL;DR: The AFLP technique provides a novel and very powerful DNA fingerprinting technique for DNAs of any origin or complexity that allows the specific co-amplification of high numbers of restriction fragments.
Journal ArticleDOI
Cryptococcosis in the era of AIDS--100 years after the discovery of Cryptococcus neoformans.
TL;DR: Clinical concepts are presented that relate to the distinctive features of cryptococcosis in patients with AIDS and the diagnosis, treatment, and prevention of Cryptococcus neoformans in AIDS patients.
Journal ArticleDOI
Biodiversity and systematics of basidiomycetous yeasts as determined by large-subunit rDNA D1/D2 domain sequence analysis.
TL;DR: The molecular systematics of 337 strains of basidiomycetous yeasts and yeast-like fungi, representing 230 species in 18 anamorphic and 24 teleomorphic genera, was determined by sequence analysis of the D1/D2 region of the large-subunit rDNA.
Patent
Selective restriction fragment amplification : a general method for DNA fingerprinting
Marc Zabeau,Pieter Vos +1 more
TL;DR: In this paper, a process for the controlled amplification of at least one part of a starting DNA containing a plurality of restriction sites for a determined specific restriction endonuclease, and of which at least part of its nucleic acid is unknown.
Journal ArticleDOI
Cryptococcosis in the acquired immunodeficiency syndrome.
Joseph A. Kovacs,Andrea Kovacs,Michael A. Polis,W. Craig Wright,Vee J. Gill,Carmelita U. Tuazon,Edward P. Gelmann,H. Clifford Lane,Robert Longfield,Gary Overturf,Abe M. Macher,Anthony S. Fauci,Joseph E. Parrillo,John E. Bennett,Henry Masur +14 more
TL;DR: Cryptococcosis in patients with the acquired immunodeficiency syndrome is a debilitating disease that does not respond to conventional therapy; earlier diagnosis or long-term suppressive therapy may improve the prognosis.