Journal ArticleDOI
Identification and characterization of a second polygalacturonase gene of Aspergillus niger
TLDR
The filamentous fungus Aspergillus niger produces several endopolygalacturonases that are involved in the degradation of pectin and PGI, the enzyme representing the second most abundant activity in a commmercial enzyme preparation, was further characterized and the corresponding gene was isolated.Abstract:
The filamentous fungus Aspergillus niger produces several endopolygalacturonases that are involved in the degradation of pectin. PGI, the enzyme representing the second most abundant activity in a commercial enzyme preparation, was further characterized and the corresponding gene was isolated. The nucleotide sequence of the pgaI gene was determined and the protein coding region was found to be interrupted by two short introns, one of which has a unusual donor splice site. The deduced 368 amino acids long protein with a putative prepropeptide of 31 amino acids shows 60% sequence identity to PGII in the mature protein. PGI overproducing A. niger strains were obtained by cotransformation with the cloned gene.read more
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Journal ArticleDOI
Aspergillus Enzymes Involved in Degradation of Plant Cell Wall Polysaccharides
Ronald P. de Vries,Jaap Visser +1 more
TL;DR: This review summarizes the current knowledge of the cell wall polysaccharide-degrading enzymes from aspergilli and the genes by which they are encoded and describes the enzymatic pathways followed by tailored modifications by using specific enzymes purified from these fungi.
Journal ArticleDOI
Fungal enzyme sets for plant polysaccharide degradation
TL;DR: This mini-review will discuss the enzymes needed for complete degradation of plant polysaccharides and will give an overview of the latest developments concerning fungal carbohydrate-active enzymes and their corresponding families.
Journal ArticleDOI
Pectin lyase: A review
TL;DR: This review tries to fill the gap by providing all relevant information exclusively for pectin lyase by covering structural aspects, substrate specificity, molecular biology, biotechnological applications and future prospects of pECTin lyases.
Journal ArticleDOI
Molecular genetic evidence for the involvement of a specific polygalacturonase, P2c, in the invasion and spread of Aspergillus flavus in cotton bolls.
Mei-Tsu Shieh,Robert L. Brown,Michael P. Whitehead,Jeffrey W. Cary,Peter J. Cotty,Thomas E. Cleveland,Ralph A. Dean +6 more
TL;DR: Results provide direct evidence that P2c contributes to the invasion and spread of A. flavus during infection of cotton bolls and other factors not evaluated in this study also contribute to aggressiveness.
Journal ArticleDOI
Recent advances in the molecular genetics of plant cell wall-degrading enzymes produced by plant pathogenic fungi
Seanna L. Annis,Paul H. Goodwin +1 more
TL;DR: A new approach using recombinant DNA techniques has been employed to try to provide more conclusive evidence concerning the role of CWDE in plant pathogenesis.
References
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TL;DR: Molecular Cloning has served as the foundation of technical expertise in labs worldwide for 30 years as mentioned in this paper and has been so popular, or so influential, that no other manual has been more widely used and influential.
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Improved M13 phage cloning vectors and host strains: nucleotide sequences of the M13mp18 and pUC19 vectors
TL;DR: New Escherichia coli host strains have been constructed for the E. coli bacteriophage M13 and the high-copy-number pUC-plasmid cloning vectors and mutations introduced into these strains improve cloning of unmodified DNA and of repetitive sequences.
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A comprehensive set of sequence analysis programs for the VAX
TL;DR: A group of programs that will interact with each other has been developed for the Digital Equipment Corporation VAX computer using the VMS operating system.
Journal ArticleDOI
Sequence from picomole quantities of proteins electroblotted onto polyvinylidene difluoride membranes.
TL;DR: Small amounts of myoglobin, beta-lactoglobulin, and other proteins and peptides can be spotted or electroblotted onto polyvinylidene difluoride membranes, stained with Coomassie Blue, and sequenced directly, suggesting that PVDF membranes are superior supports for sequence analysis of picomole quantities of proteins purified by gel electrophoresis.
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Basic methods in molecular biology
TL;DR: General methods bacterial strains and cloning vectors enzymes that modify DNA and RNA in vitro amplification of DNA using the polymerase chain reaction (PCR) and the thermostable Taq DNA polymerase, introduction DNA restriction fragment analysis and preparation.