Journal ArticleDOI
Identification of a protein that purifies with the scrapie prion.
TLDR
Initial results suggest that the amount of this protein correlates with the titer of the agent, and the resistance of thisprotein to digestion by proteinase K distinguished it from proteins of similar molecular weight found in normal hamster brain.Abstract:
Purification of prions from scrapie-infected hamster brain yielded a protein that was not found in a similar fraction from uninfected brain. The protein migrated with an apparent molecular size of 27,000 to 30,000 daltons in sodium dodecyl sulfate polyacrylamide gels. The resistance of this protein to digestion by proteinase K distinguished it from proteins of similar molecular weight found in normal hamster brain. Initial results suggest that the amount of this protein correlates with the titer of the agent.read more
Citations
More filters
Journal ArticleDOI
Mice devoid of PrP are resistant to scrapie
Hansruedi Büeler,Adriano Aguzzi,Andreas W. Sailer,R.-A. Greiner,P. Autenried,Michel Aguet,Charles Weissmann +6 more
TL;DR: These experiments show that PrPC, possibly at close to normal levels, is required for the usual susceptibility to scrapie and that lack of homology between incoming prions and the host's PrP genes retards disease.
Journal ArticleDOI
Molecular biology of prion diseases
TL;DR: Understanding prion diseases may advance investigations of other neurodegenerative disorders and of the processes by which neurons differentiate, function for decades, and then grow senescent.
Journal ArticleDOI
A cellular gene encodes scrapie PrP 27-30 protein
Bruno Oesch,Bruno Oesch,David Westaway,Monika Wälchli,Monika Wälchli,Michael P. McKinley,Stephen B. H. Kent,Stephen B. H. Kent,Ruedi Aebersold,Ruedi Aebersold,Ronald A. Barry,Paul Tempst,Paul Tempst,David B. Teplow,David B. Teplow,Leroy Hood,Leroy Hood,Stanley B. Prusiner,Stanley B. Prusiner,Charles Weissmann,Charles Weissmann +20 more
TL;DR: A clone encoding PrP 27-30, the major protein in purified preparations of scrapie agent, was selected from a scrapie-infected hamster brain cDNA library by oligonucleotide probes corresponding to the N terminus of the protein.
Journal ArticleDOI
Unfolding the role of protein misfolding in neurodegenerative diseases.
TL;DR: The aim of this article is to review the literature on the molecular mechanism of protein misfolding and aggregation, its role in Neurodegeneration and the potential targets for therapeutic intervention in neurodegenerative diseases.
References
More filters
Journal ArticleDOI
Cleavage of Structural Proteins during the Assembly of the Head of Bacteriophage T4
TL;DR: Using an improved method of gel electrophoresis, many hitherto unknown proteins have been found in bacteriophage T4 and some of these have been identified with specific gene products.
Journal Article
Cleavage of structural proteins during the assemble of the head of bacterio-phage T4
TL;DR: Using an improved method of gel electrophoresis, many hitherto unknown proteins have been found in bacteriophage T4 and some of these have been identified with specific gene products as mentioned in this paper.
Journal ArticleDOI
A simplification of the protein assay method of Lowry et al. which is more generally applicable
TL;DR: A simple method based on a linear log-log protein standard curve is presented to permit rapid and totally objective protein analysis using small programmable calculators.
Journal ArticleDOI
A Film Detection Method for Tritium‐Labelled Proteins and Nucleic Acids in Polyacrylamide Gels
TL;DR: A simple method for detecting 3H in polyacrylamide gels by scintillation autography (fluorography) using X-ray film, which is ten times more sensitive than conventional autoradiography of isotopes with higher emission energies.
Journal ArticleDOI
Novel proteinaceous infectious particles cause scrapie
TL;DR: A new term "prion" is proposed to denote a small proteinaceous infectious particle which is resistant to inactivation by most procedures that modify nucleic acids.
Related Papers (5)
A cellular gene encodes scrapie PrP 27-30 protein
Bruno Oesch,Bruno Oesch,David Westaway,Monika Wälchli,Monika Wälchli,Michael P. McKinley,Stephen B. H. Kent,Stephen B. H. Kent,Ruedi Aebersold,Ruedi Aebersold,Ronald A. Barry,Paul Tempst,Paul Tempst,David B. Teplow,David B. Teplow,Leroy Hood,Leroy Hood,Stanley B. Prusiner,Stanley B. Prusiner,Charles Weissmann,Charles Weissmann +20 more