Journal ArticleDOI
Identification of apple cultivars using RAPD markers.
TLDR
A key is proposed by which one can differentiate apple cultivars using commercially available prime by using randomly amplified polymorphic DNA markers obtained by the polymerase chain reaction (PCR).Abstract:
Eleven apple cultivars were differentiated using randomly amplified polymorphic DNA (RAPD) markers obtained by the polymerase chain reaction (PCR). The variability of the technique and of the origin of the DNA extract was analyzed. A set of bands consistent in their presence or absence was chosen to create a differentiating band pattern. A key is proposed by which one can differentiate apple cultivars using commercially available prime.read more
Citations
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DNA fingerprinting in plants: principles, methods, and applications
TL;DR: Amin et al. as discussed by the authors used radioisotopes to measure the diversity of microsatellites in the field of plant DNA collection and preservation of plant Tissue in the Field.
Journal ArticleDOI
The random amplified polymorphic DNA (RAPD) assay and related techniques applied to genotoxicity and carcinogenesis studies: a critical review.
TL;DR: After proper optimisation, the RAPD is a reliable, sensitive and reproducible assay, has the potential to detect a wide range of DNA damage as well as mutations and therefore can be applied to genotoxicity and carcinogenesis studies and is suggested that these assays would continue to complement the use of emerging technologies.
Journal ArticleDOI
Microsatellites in Malus X domestica (apple): abundance, polymorphism and cultivar identification
Parry Guilford,S. Prakash,J. M. Zhu,Erik H. A. Rikkerink,Susan E. Gardiner,H. Bassett,Richard L.S. Forster +6 more
TL;DR: Screening of an apple genomic library with (GA)15 and (GT)15 probes demonstrated that these repeats are abundant, occurring about every 120 and 190 kb, respectively.
Journal ArticleDOI
Molecular Marker Linkage Map for Apple
TL;DR: The double pseudotestcross format of the mapping population permitted the comparison of recombination frequencies in male and female parents in certain regions of the genome where appropriate markers were available and gave no indication that a sex-related difference in recombination rate was characteristic of apple.
Journal ArticleDOI
PCR-based fingerprinting using AFLPs as a tool for studying genetic relationships in Lactuca spp
TL;DR: AFLP markers were evaluated for determining the phylogenetic relationships Lactuca spp.
References
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Journal ArticleDOI
DNA polymorphisms amplified by arbitrary primers are useful as genetic markers
TL;DR: A new DNA polymorphism assay based on the amplification of random DNA segments with single primers of arbitrary nucleotide sequence is described, suggesting that these polymorphisms be called RAPD markers, after Random Amplified Polymorphic DNA.
Journal ArticleDOI
A plant DNA minipreparation: Version II
TL;DR: The topic of this report is rap,d m,croscale methods for,solat,on of plant D N A without tile use of ul tracentr ,fugatlon wEth CsCI, which is of moderately high molecular weight and serves as a satisfactory substrate for most restrlctum cndonucleases and is statable for genom,c blot analys,s.
Journal ArticleDOI
Fingerprinting genomes using PCR with arbitrary primers
John Welsh,Michael McClelland +1 more
TL;DR: The generality of the arbitrarily primed PCR method is demonstrated by application to twenty four strains from five species of Staphylococcus, eleven strains of Streptococcus pyogenes and three varieties of Oryza sativa.
Journal ArticleDOI
Polymorphisms generated by arbitrarily primed PCR in the mouse: application to strain identification and genetic mapping.
TL;DR: The method will allow rapid genetic mapping of DNA polymorphisms without Southern blotting and genetically mapped four polymorphisms in a set of C57BL/6J x DBA/2J recombinant inbreds.
Journal ArticleDOI
Rapid identification of genetic variation and pathotype of Leptosphaeria maculans by random amplified polymorphic DNA assay.
P H Goodwin,S L Annis +1 more
TL;DR: Canadian isolates of Leptosphaeria maculans, the causal agent of blackleg of crucifers, were examined for genetic relatedness by the random amplified polymorphic DNA assay and results agreed with other reports which showed many genetic differences between pathotypes.
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