Journal ArticleDOI
Identification of differentially expressed genes in seeds of two near-isogenic Brassica napus lines with different oil content
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TLDR
In this paper, the authors compared gene expression during seed development between two lines of Brassica napus with a 10% difference in oil content, and found that six genes were highly expressed in seeds with low oil content.Abstract:
The regulation of seed oil synthesis in rapeseed is largely unknown. In this study, we compared the gene expression during seed development between two lines of Brassica napus with a 10% difference in oil content. We isolated the immature seeds 15 and 25 days after flowering at periods preceding and including the major accumulation of storage oils and proteins. The differentially expressed gene clones between the two rape lines were isolated by subtractive suppression hybridization (SSH). All SSH clones were arrayed and screened by dot blot hybridization, followed by RT-PCR analysis for selected clones. A total of 217 cDNA clones corresponding to 30 genes were found to have a high expression in seeds with high oil content. Six genes were highly expressed in seeds with low oil content. Northern blot and enzyme activity analysis demonstrated a change in expression pattern of several genes. The results provide information on gene-encoding factors responsible for the regulation of oil synthesis. The possible role of these genes in seeds is discussed. The genes in this study may be suitable as novel targets for genetic improvement of seed oil content and may also provide molecular markers for studies of rape breeding.read more
Citations
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Journal ArticleDOI
In Posidonia oceanica cadmium induces changes in DNA methylation and chromatin patterning
TL;DR: The data demonstrate that Cd perturbs the DNA methylation status through the involvement of a specific methyltransferase, linked to nuclear chromatin reconfiguration likely to establish a new balance of expressed/repressed chromatin.
Journal ArticleDOI
Increasing the flow of carbon into seed oil.
Randall J. Weselake,David C. Taylor,M. Habibur Rahman,Saleh Shah,André Laroche,Peter B. E. McVetty,John L. Harwood +6 more
TL;DR: The demand for vegetable oils for food, fuel (bio-diesel) and bio-product applications is increasing rapidly and it is estimated that a 50 to 75% increase in canola oil production will be required to meet the demand for seed oil in the next 7-10years.
Journal ArticleDOI
Comparative deep transcriptional profiling of four developing oilseeds
Manuel Adrián Troncoso-Ponce,Aruna Kilaru,Xia Cao,Xia Cao,Timothy P. Durrett,Timothy P. Durrett,Jilian Fan,Jacob Krüger Jensen,Nicholas Thrower,Markus Pauly,Markus Pauly,Curtis G. Wilkerson,Curtis G. Wilkerson,John B. Ohlrogge,John B. Ohlrogge +14 more
TL;DR: Using pyrosequencing, over 7 million ESTs from four stages of developing seeds of Ricinus communis, Brassica napus, Euonymus alatus and Tropaeolum majus are generated, revealing both conserved and distinct species-specific expression patterns for genes involved in the synthesis of glycerolipids and their precursors.
Journal ArticleDOI
Maternal control of seed oil content in Brassica napus: the role of silique wall photosynthesis
TL;DR: In this paper, a potential regulatory mechanism for elevated photosynthesis in the zy036 silique wall is proposed on the basis of knowledge from Arabidopsis, and the results highlight an important role of silique-wall photosynthesis for the regulation of seed oil content in terms of maternal effects.
Journal ArticleDOI
The BnGRF2 gene (GRF2-like gene from Brassica napus) enhances seed oil production through regulating cell number and plant photosynthesis
Jing Liu,Wei Hua,Hongli Yang,Gaomiao Zhan,Rong-Jun Li,Linbin Deng,Xinfa Wang,Guihua Liu,Hanzhong Wang +8 more
TL;DR: Improvement to economically important characteristics in oil crops may be achieved by manipulation of the GRF2 expression level by differential gene expression analyses between rapeseed lines.
References
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Journal ArticleDOI
Suppression subtractive hybridization: a method for generating differentially regulated or tissue-specific cDNA probes and libraries
Luda Diatchenko,Yun-Fai Chris Lau,Aaron P. Campbell,Alex Chenchik,Fauzia Moqadam,Betty C. B. Huang,Sergey Lukyanov,Konstantin A. Lukyanov,Nadya G. Gurskaya,E.D. Sverdlov,Paul D. Siebert +10 more
TL;DR: The results suggest that the SSH technique is applicable to many molecular genetic and positional cloning studies for the identification of disease, developmental, tissue-specific, or other differentially expressed genes.
Journal ArticleDOI
Regulation of fatty acid synthesis
John B. Ohlrogge,Jan G. Jaworski +1 more
TL;DR: This review evaluates current knowledge of regulation of plant fatty metabolism and attempts to identify the major unanswered questions.
Journal ArticleDOI
WRINKLED1 encodes an AP2/EREB domain protein involved in the control of storage compound biosynthesis in Arabidopsis
Alex Cernac,Christoph Benning +1 more
TL;DR: Oil-accumulating seedlings showed aberrant development consistent with a prolonged embryonic state, and the putative AP2/EREBP transcription factor WRINKLED1 (WRI1) is involved in the regulation of seed storage metabolism in Arabidopsis.
Journal ArticleDOI
Contrapuntal Networks of Gene Expression during Arabidopsis Seed Filling
TL;DR: This article used microarray data to examine changes in gene expression during Arabidopsis seed development and compared wild-type and mutant wrinkled1 ( wri1 ) seeds that have an 80% reduction in oil.
Journal ArticleDOI
Seed-Specific Over-Expression of an Arabidopsis cDNA Encoding a Diacylglycerol Acyltransferase Enhances Seed Oil Content and Seed Weight
Colette Jako,Arvind Kumar,Yangdou Wei,Jitao Zou,Dennis L. Barton,E. Michael Giblin,Patrick S. Covello,David C. Taylor +7 more
TL;DR: The current study confirms the important role of DGAT in regulating the quantity of seed triacylglycerols and the sink size in developing seeds and shows for the first time that seed-specific over-expression of the DGAT cDNA in wild-type Arabidopsis enhances oil deposition and average seed weight, which are correlated with DGAT transcript levels.
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