Identification of gh|alui and ghr|alui genes polymorphisms in indonesian buffalo
TLDR
Results showed no polymorphisms were detected in these genes in buffalo, and all buffaloes tested had LL genotype forlocus GH|AluI and AA genotypes for locus GHR|ALUI.Abstract:
Growth hormone (GH) is an anabolic hormone which sintesized and secreted by somatrotop cell inpituitary anterior lobe. GH exert its effect on growth and metabolism by interacting with a specificreceptor on the surface of the target cells. Growth hormone receptor (GHR) has been suggested ascandidate gene for traits related to meat production in Bovidae. The objectives of this study were toidentify polymorphism of GH and GHR genes in buffalo. The 452 DNA samples buffalo were collectedfrom five populations in Indonesia (Siborong-Borong-Medan (65), Lebak-Banten (29), Pandeglang-Banten (180), Semarang-Central Java, and Mataram-West Nusa Tenggara (103)). A gene fragment of theGH|AluI gene at 432 bp located on exon 3 and GHR|AluI gene at 298 bp on exon 10 were successfullyamplified by using the techniques of a PCR (polymerase chain reaction) and genotyped by PCR-RFLP(restriction fragment length polymorphism) then -SSCP (single strand conformation polymorphism). Theresults showed no polymorphisms were detected in these genes. All buffaloes tested had LL genotype forlocus GH|AluI and AA genotype for locus GHR|AluI.read more
Citations
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Journal Article
Molecular dissection of a quantitative trait locus: a phenylalanine-to-tyrosine substitution in the transmembrane domain of the bovine growth hormone receptor is associated with a major effect on milk yield and composition.
Wouter Coppieters,Sarah C. Blott,Michel Georges,Richard J. Spelman,Latifa Karim,Frédéric Farnir,Anne Lisbeth Schmidt,Bernard Grisart,Anne Cornet,Sirja Moisio,Patricia Simon-Assmann,Nadine Cambisano,Sharon A Küntzel,Talitha C. Ford,Chang-Joo Kim,Jerry Wong,Russell G. Snell,Gwang Sik Kim,Dave Johnson,Paulette Berzi,Johanna Vilkki +20 more
TL;DR: Using a denser chromosome 20 marker map and exploiting linkage disequilibrium using two distinct approaches, strong evidence is provided that a chromosome segment including the gene coding for the growth hormone receptor accounts for at least part of the chromosome 20 QTL effect.
Journal ArticleDOI
Investigation of Growth Hormone Releasing Hormone, Growth Hormone and Prolactin Hormone Gene Polymorphism in Anatolian Water Buffalo
Mehmet Akif Konca,Bilal Akyüz +1 more
TL;DR: This study is the first to examine GHRH/HaeIII, GH/AluI and PRL/RsaI polymorphisms in Anatolian water buffalo by means of the PCR-RFLP method.
Journal ArticleDOI
Effects of genetic polymorphism in Pit1, GH, GHR and KCN3 on milk yield and body weight of Khuzestan (Iran) water buffaloes
TL;DR: The results suggest that devising an integrated breeding plan in Khuzestan water buffalos can considerably benefit from the very high diversity in candidate genes.
Journal ArticleDOI
The Variability of Growth Hormone Gene Associated with Ultrasound Imaging of Longissimus dorsi Muscle and Perirenal Fat in Rabbits
TL;DR: The association of characteristic genotype of GH| Bsh1236I gene with measurement phenotype was not significant, however it had potency as marker assisted selection (MAS).
Journal ArticleDOI
Histomorphology and Physical Characteristics of Buffalo Meat at Different Sex and Age
TL;DR: The result showed that muscle fiber diameter was influenced by ages, and meat qualities of buffaloes were not affected by age (2-4 yr) and sex.
References
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Book
Molecular Evolution and Phylogenetics
Masatoshi Nei,Sudhir Kumar +1 more
TL;DR: This chapter discusses the molecular basis of evolution, the evolution of organisms based on the fossil record, and the implications of these events for phylogenetic inference.
Journal ArticleDOI
PCR-SSCP: a simple and sensitive method for detection of mutations in the genomic DNA.
Book
World watch list for domestic animal diversity
TL;DR: The World watch list for domestic animal diversity is a list of countries where dogs, cats, foxes and other animals have been put on a watch list by the United Nations Educational, Scientific and Cultural Organization (UNESCO) for the purposes of promoting animal welfare.
Journal Article
Molecular dissection of a quantitative trait locus: a phenylalanine-to-tyrosine substitution in the transmembrane domain of the bovine growth hormone receptor is associated with a major effect on milk yield and composition.
Wouter Coppieters,Sarah C. Blott,Michel Georges,Richard J. Spelman,Latifa Karim,Frédéric Farnir,Anne Lisbeth Schmidt,Bernard Grisart,Anne Cornet,Sirja Moisio,Patricia Simon-Assmann,Nadine Cambisano,Sharon A Küntzel,Talitha C. Ford,Chang-Joo Kim,Jerry Wong,Russell G. Snell,Gwang Sik Kim,Dave Johnson,Paulette Berzi,Johanna Vilkki +20 more
TL;DR: Using a denser chromosome 20 marker map and exploiting linkage disequilibrium using two distinct approaches, strong evidence is provided that a chromosome segment including the gene coding for the growth hormone receptor accounts for at least part of the chromosome 20 QTL effect.
Journal ArticleDOI
Molecular dissection of a quantitative trait locus: a phenylalanine-to-tyrosine substitution in the transmembrane domain of the bovine growth hormone receptor is associated with a major effect on milk yield and composition.
Sarah C. Blott,Jong-Joo Kim,Sirja Moisio,Anne Schmidt-Kuntzel,Anne Cornet,Paulette Berzi,Nadine Cambisano,CA Ford,Bernard Grisart,Dave Johnson,Latifa Karim,Patricia Simon,Russell G. Snell,Richard J. Spelman,Jerry Wong,Johanna Vilkki,Michel Georges,Frédéric Farnir,Wouter Coppieters +18 more
TL;DR: In this article, the authors used a denser chromosome 20 marker map and exploited linkage disequilibrium using two distinct approaches to provide strong evidence that a chromosome segment including the gene coding for the growth hormone receptor accounts for at least part of the chromosome 20 QTL effect.
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