Image-guided focused ultrasound modulates electrically evoked motor neuronal activity in the mouse peripheral nervous system in vivo.
Min Gon Kim,Hermes A. S. Kamimura,Stephen A. Lee,Christian Aurup,Nancy Kwon,Elisa E. Konofagou +5 more
TLDR
It is demonstrated that image-guided FUS can selectively modulate motor neuron activity in the mouse sciatic nerve in vivo and attribute motor responses to thermal effects.Abstract:
Objective. Focused ultrasound (FUS) has recently been demonstrated capable of exciting motor neuronal activity. However, comprehensive understanding of elucidated excitatory and inhibitory effects is required to better assess FUS-mediated modulation. In this study, we demonstrate that image-guided FUS can selectively modulate motor neuron activity in the mouse sciatic nerve in vivo and attribute motor responses to thermal effects. Approach. FUS was applied on the sciatic nerve of anesthetized mice in vivo through the intact skin and muscle using ultrasound imaging for targeting. Both excitatory and inhibitory effects were recorded using electromyography (EMG) along with muscle response of the hind limb. The effects of FUS modulation vs heating by invasive alternative heating source (AHS) on electrically evoked EMG responses in the sciatic nerve in vivo were also investigated. The safety and reversibility of the technique were validated using histology and EMG recovery. Main results. The FUS was capable of eliciting motor neuronal activity comparable to electrical stimulation, and facilitating motor neuronal response on electrically evoked potentials with temperature elevation up to 11.5 ± 0.3 oC (PRF ≤ 40 Hz). On the other hand, FUS-induced temperature elevations above 15.1 ± 1.6 oC (PRF ≥ 100 Hz) resulted in the suppression of electrically-evoked motor neuronal activity along with a decrease in EMG latency and area under the curve (AUC), which was validated against the invasive AHS with temperature elevation of 18.1 ± 8.5 oC instead of FUS modulation. Histological findings along with EMG responses after FUS modulation demonstrated a reversible or irreversible modulation. Significance. The findings reported herein indicate that image-guided FUS (PRF ≤ 100 Hz) induces safe and controllable modulation of involuntarily evoked motor neuron activity in vivo.read more
Citations
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Journal ArticleDOI
Ultrasound Technologies for Imaging and Modulating Neural Activity
Claire Rabut,Sangjin Yoo,Robert C. Hurt,Zhiyang Jin,Hongsyi Li,Hongsun Guo,Bill Ling,Mikhail G. Shapiro +7 more
TL;DR: The state of the art and ongoing developments in ultrasonic neurotechnology are reviewed, building from fundamental principles to current utility, open questions, and future potential.
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Ultrasound neuromodulation: mechanisms and the potential of multimodal stimulation for neuronal function assessment.
TL;DR: The mechanisms and their potential overlap are reviewed along with discussions on the potential insights into mechanisms that magnetic resonance imaging sequences along with a multimodal stimulation approach involving electrical, magnetic, chemical, light, and mechanical stimuli can provide.
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Emerging Frontier of Peripheral Nerve and Organ Interfaces.
TL;DR: The innovations in bioelectronics borne out of interdisciplinary collaborations between biologists and physical scientists may not only advance fundamental study of peripheral physiology but also empower clinical interventions for conditions including neurological, gastrointestinal, and immune dysfunction.
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A modular strategy for next-generation upper-limb sensory-motor neuroprostheses
Solaiman Shokur,Solaiman Shokur,Alberto Mazzoni,Giuseppe Schiavone,Douglas J. Weber,Silvestro Micera,Silvestro Micera +6 more
TL;DR: It is argued that any neuroprosthesis relies on a set of hardware and algorithmic building elements that are called neurotechnological modules used for motor decoding, movement restoration, or sensory feedback, and how the modular approach is already present in current Neuroprosthetic solutions and how it can further exploit it to imagine the next generation of neuroProsthetics for sensory-motor restoration.
Journal ArticleDOI
Focused ultrasound excites action potentials in mammalian peripheral neurons in part through the mechanically gated ion channel PIEZO2
Benjamin U. Hoffman,Yoshichika Baba,Stephen A. Lee,Chi-Kun Tong,Elisa E. Konofagou,Ellen A. Lumpkin +5 more
TL;DR: This study identifies reliable protocols and molecular mechanisms for stimulating action potentials from individual peripheral neurons in the mammalian nervous system and reveals the translational potential of ultrasound to effectively modulate the PNS through intrinsic neuronal mechanisms.
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