Journal ArticleDOI
Immobilization of Candida antarctica lipase B by covalent attachment on chitosan-based hydrogels using different support activation strategies
James A. Silva,G.P. Macedo,D. S. Rodrigues,Raquel L. C. Giordano,Luciana Rocha Barros Gonçalves +4 more
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TLDR
Candida antarctica lipase B immobilization by covalent attachment on chitosan and on ch itosan–alginate complex previously activated by different strategies was studied.About:
This article is published in Biochemical Engineering Journal.The article was published on 2012-01-15. It has received 130 citations till now. The article focuses on the topics: Immobilized enzyme & Candida antarctica.read more
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3-D magnetic graphene oxide-magnetite poly(vinyl alcohol) nanocomposite substrates for immobilizing enzyme
Li Yanyun,Tao Jing,Gaofeng Xu,Jingzhi Tian,Mengyao Dong,Qian Shao,Bin Wang,Zhikang Wang,Yong-Jie Zheng,Changlong Yang,Zhanhu Guo +10 more
TL;DR: In this paper, three-dimensional magnetic graphene oxide-magnetite polyvinyl alcohol (3D-GO/PVA/Fe3O4) nanocomposites were successfully prepared for porcine pancreatic lipase (PPL) enzyme immobilization.
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Immobilization of laccase via adsorption onto bimodal mesoporous Zr-MOF
TL;DR: In this article, a bimodal micro-mesoporous Zr-metal organic framework (Zr-MOF, MMU) with a particle size of approximately 200nm was synthesized.
Journal ArticleDOI
Evaluation of different lipase biocatalysts in the production of biodiesel from used cooking oil: Critical role of the immobilization support
Veymar G. Tacias-Pascacio,Jose J. Virgen-Ortíz,Mauricio Jiménez-Pérez,Malcolm Yates,Beatriz Torrestiana-Sanchez,Arnulfo Rosales-Quintero,Roberto Fernandez-Lafuente +6 more
TL;DR: A new battery of biocatalyst was evaluated in the synthesis of biodiesel using a mix of used cooking oil and fats and methanol as substrates, and results clearly showed that the methyl ester yields and specific activities greatly depend on the support.
Journal ArticleDOI
Preparation of superparamagnetic Fe3O4@alginate/chitosan nanospheres for Candida rugosa lipase immobilization and utilization of layer-by-layer assembly to enhance the stability of immobilized lipase.
TL;DR: After studying the properties of ICRL such as activity, kinetic behaviors, stability and reusability, it was proved that the ICRL prepared with two methods displayed more excellent properties than that prepared with electrostatic adsorption only.
Journal ArticleDOI
Kinetic resolution of drug intermediates catalyzed by lipase B from Candida antarctica immobilized on immobead‐350
Maísa Pessoa Pinheiro,Nathalia Saraiva Rios,Thiago de Sousa Fonseca,Francisco de Aquino Bezerra,Enrique Rodríguez-Castellón,Roberto Fernandez-Lafuente,Marcos Carlos de Mattos,José C. S. dos Santos,Luciana Rocha Barros Gonçalves +8 more
TL;DR: The CALB‐IB‐350 thermal and solvent stabilities were higher than that of the soluble enzyme and the enantiomeric excess of the product was 97%.
References
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Production, purification, characterization, and applications of lipases.
TL;DR: The production, recovery, and use of microbial lipases are discussed; issues of enzyme kinetics, thermostability, and bioactivity are addressed; and production of recombinant lipases is detailed.
Journal ArticleDOI
Aldehyde-agarose gels as activated supports for immobilization-stabilization of enzymes
TL;DR: Aldehyde groups, moderately separated from support surfaces, are proposed as suitable active groups for developing strategies to insolubilize-stabilize enzymes by multipoint covalent attachment to activated preexistent supports.
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Production of biodiesel fuel from triglycerides and alcohol using immobilized lipase
TL;DR: In this article, a transesterification reaction was performed using triglycerides and short-chain alcohol by immobilized lipase in non-aqueous conditions and the long-chain fatty acid ester, which is the product of this reaction, can be used as a diesel fuel that does not produce sulfur oxide and minimize the soot particulate.
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Bioreactors with immobilized lipases: state of the art.
TL;DR: This review attempts to provide an updated compilation of studies reported in the literature pertaining to reactors containing lipases in immobilized forms, in a way that helps the reader direct a bibliographic search and develop an integrated perspective of the subject.
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Interfacial adsorption of lipases on very hydrophobic support (octadecyl-Sepabeads): Immobilization, hyperactivation and stabilization of the open form of lipases
Jose M. Palomo,Gloria Muñoz,Gloria Fernández-Lorente,Cesar Mateo,Roberto Fernandez-Lafuente,Jose M. Guisan +5 more
TL;DR: It seems that the “open structure” of lipases, adsorbed on hydrophobic supports, is much more active and much more stable than the corresponding “closed” structure even when the closed structure is undergoing a very intense multipoint covalent attachment.