Journal ArticleDOI
Immobilization of proteins on self-assembled monolayers.
TLDR
It is found that BSA and LYZ can be readily immobilized on SAMs at their isoelectric point (IEP), and the strong hydrophobic interaction at the IEP is attributed to immobilization.Abstract:
The immobilization of protein molecules on self-assembled monolayers (SAM) by physical interactions and chemical bonding has been studied using atomic force microscopy (AFM). The proteins used for our investigation are bovine serum albumin (BSA), lysozyme (LYZ), and normal rabbit immunoglobulin G (IgG). The surfaces are methyl-, hydroxyl-, carboxylic acid- and aldehyde-terminated SAMs. We found that BSA and LYZ can be readily immobilized on SAMs at their isoelectric point (IEP). The detailed surface morphology of adsorbed proteins varies with the functionality of the SAMs. The strong hydrophobic interaction at the IEP is attributed to immobilization. If the solution pH is deviated from the IEP, proteins may be attached onto the surface via electrostatic interactions. Covalent binding between the aldehyde-terminated SAM and the H2N-groups in the protein results in immobilization of all three proteins. The individual proteins and their orientations on SAMs are clearly resolved from high-resolution AFM images. The stability and bioactivity of these immobilized proteins are also studied.read more
Citations
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Journal ArticleDOI
AFM: a versatile tool in biophysics
Andrea Alessandrini,Paolo Facci +1 more
TL;DR: It is hoped that this review can help the reader in appreciating how atomic force microscopy contributes to the current explosive growth of nanobiosciences, where biology, chemistry and physics merge.
Journal ArticleDOI
Peptide-based Biopolymers in Biomedicine and Biotechnology
TL;DR: Peptides are emerging as a new class of biomaterials due to their unique chemical, physical, and biological properties, and applications in protein purification, controlled drug delivery, tissue engineering, and biosurface engineering are discussed.
Journal ArticleDOI
Protein Adsorption on Model Surfaces with Controlled Nanotopography and Chemistry
Frédéric A. Denis,Per Hanarp,Duncan S. Sutherland,Julie Gold,Christian Mustin,Paul Rouxhet,Yves F. Dufrêne +6 more
TL;DR: In this article, the authors evaluated the influence of substrate surface characteristics on protein adsorption processes and concluded that the supramolecular organization of the adsorbed layer is controlled both by surface chemistry and topography.
Journal ArticleDOI
New insights for self-assembled monolayers of organothiols on Au(111) revealed by scanning tunneling microscopy
Guohua Yang,Gang-yu Liu +1 more
TL;DR: In this paper, a molecular-level understanding of the formation of adatom and vacancy islands and how the structure is impacted by introducing aromatic termini is discussed, and thermal stability and thermally induced structural evolution of alkanethiol molecules under local electric field and tunneling current are studied with molecular resolution.
Journal ArticleDOI
An overview of the biophysical applications of atomic force microscopy.
TL;DR: AFM is progressively becoming a usual benchtop technique and overcomes materials science applications, showing that 17 years after its invention, AFM has completely crossed the limits of its traditional areas of application.
References
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Journal ArticleDOI
Formation of monolayer films by the spontaneous assembly of organic thiols from solution onto gold
Colin D. Bain,E. Barry Troughton,Yu-Tai Tao,Joseph Evall,George M. Whitesides,Ralph G. Nuzzo +5 more
TL;DR: In this paper, the authors used contact angles and optical ellipsometry to study the kinetics of adsorption of monolayer films and to examine the experimental conditions necessary for the formation of high-quality films.
Journal ArticleDOI
Adsorption of proteins onto surfaces containing end-attached oligo(ethylene oxide): a model system using self-assembled monolayers
TL;DR: In this paper, the adsorption of four proteins-fibrinogen, lysozyme, pyruvate kinase, and RNAse A-to self-assembled monolayers (SAMs) on gold was studied.
Journal ArticleDOI
Using self-assembled monolayers to understand the interactions of man-made surfaces with proteins and cells.
TL;DR: The convenience and broad application offered by SAMs and microcontact printing make this combination of techniques useful for studying a variety of fundamental phenomena in biointerfacial science.