Journal ArticleDOI
In-beam electron impact mass spectrometry of N-carbobenzoxy derivatives of oligopeptides composed of leucine and isoleucine
Mamoru Ohashi,Kazuo Tsujimoto,Shigeki Tamura,Noboru Nakayama,Yasuaki Okumura,Atsushi Sakurai +5 more
TLDR
From the studies on temperature spectral change of in-beam electron impact spectra it became clear that rapid heating of the sample over the temperature range 200-300 degrees C gave abundant [M + 1]+ ions as well as fragment ions originating from the thermally intact peptide as the major peaks in the spectrum.Abstract:
The characteristics of the in-beam electron impact mass spectra of 13 N-carbobenzoxyoligopeptides (up to pentapeptides) composed mainly of leucine and isoleucine, and their alkyl and N-hydroxysuccinimide esters are described. In the cases of di- and tripeptide derivatives, each in-beam electron impact spectrum exhibited an abundant [M + 1]+ peak as well as amino-acyl fragments. However, in the case of pentapeptides the in-beam spectra measured under the routine conditions corresponded to the conventional electron impact spectra of the cyclopeptide derivatives formed by thermal cyclization in the mass spectrometer and few [M + 1]+ ions of the original peptides were observed. From the studies on temperature spectral change of in-beam electron impact spectra it became clear that rapid heating of the sample over the temperature range 200-300 degrees C gave abundant [M + 1]+ ions as well as fragment ions originating from the thermally intact peptide as the major peaks in the spectrum.read more
Citations
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Journal ArticleDOI
Toxicity of nitro compounds fromLotus pedunculatus to grass grub (Costelytra zealandica) (Coleoptera: Scarabaeidae).
R. F. N. Hutchins,Oliver R. W. Sutherland,C. Gnanasunderam,W. J. Greenfield,E. M. Williams,H. J. Wright +5 more
TL;DR: Crude extracts of root of the forage legumesLotus pedunculatus andCoronilla varia (crownvetch) were toxic when administered orally to 3rd instarCostelytra zealandica larvae and a group of 3-nitropropanoyl-d-glucopyranoses was isolated from active fractions of the crude extracts.
Journal ArticleDOI
Thermal degradation of O‐acyl oximes under electron impact ionization conditions
Journal ArticleDOI
Matrix‐Assisted in‐Beam Mass Spectra of Thermally Fragile Molecules
Lilian Kao Liu,Tsing-Pai Hsieh +1 more
TL;DR: In-beam and matrix-isolation techniques have been used in the mass spectral studies of several categories of biologically significant compounds, such as amino acids, quaternary ammonium salts, vitamins and nucleosides as mentioned in this paper.
Book ChapterDOI
Chapter 3 Mass spectrometry
J.C. Tabet,M. Fétizon +1 more
TL;DR: The presence of easily detected and intense fragmentations led to the possibility of studying complex mixtures quantitatively with techniques such as selected ion monitoring (SIM) and multiple ion detection (MID), which are especially when the mass spectrometer is coupled to a gas chromatograph.
Book ChapterDOI
The Development of the Solids Probe for EI-MS
TL;DR: In this article, a vacuum lock was also developed for the introduction of the tip of the solids probe to the ion source without having to break the source vacuum, which was used to introduce compounds with molecular weights within the range of the mass spectrometer.
References
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Journal ArticleDOI
The Use of Esters of N-Hydroxysuccinimide in Peptide Synthesis
Journal ArticleDOI
A Method of Synthesis of Long Peptide Chains Using a Synthesis of Oxytocin as an Example
Journal ArticleDOI
Structure revision of the antibiotic echinomycin.
Anne Dell,Dudley H. Williams,Howard R. Morris,Gerald A. Smith,James Feeney,Gordon C. K. Roberts +5 more
TL;DR: In the light of evidence based on ’H and I3C nuclear magnetic resonance experiments, and on electron impact and field desorption mass spectrometry, the structure of the antibiotic echinomycin has been revised.
Journal ArticleDOI
Proton transfer mass spectrometry of peptides. A rapid heating technique for underivatized peptides containing arginine.
Journal ArticleDOI
Direct chemical ionization of relatively involatile samples. Application to underivatized oligopeptides
M. A. Baldwin,Fred W. McLafferty +1 more
TL;DR: By direct explosure of the sample to the ion plasma in a chemical ionization source, spectra of many relatively involatile compounds can be obtained with sample temperatures at least 150° below those usually required.
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