In Vivo Quantitative Vasculature Segmentation and Assessment for Photodynamic Therapy Process Monitoring Using Photoacoustic Microscopy
Thi Thao Mai,Su Woong Yoo,Suhyun Park,Jin Young Kim,Kang-Ho Choi,Chulhong Kim,Seong Young Kwon,Jung-Joon Min,Changho Lee +8 more
TLDR
In this paper, the authors investigated the feasibility of label-free quantitative photoacoustic microscopy (PAM) for monitoring the vasculature changes under the effect of photodynamic therapy (PDT) in mouse ear melanoma tumors.Abstract:
Vascular damage is one of the therapeutic mechanisms of photodynamic therapy (PDT). In particular, short-term PDT treatments can effectively destroy malignant lesions while minimizing damage to nonmalignant tissue. In this study, we investigate the feasibility of label-free quantitative photoacoustic microscopy (PAM) for monitoring the vasculature changes under the effect of PDT in mouse ear melanoma tumors. In particular, quantitative vasculature evaluation was conducted based on Hessian filter segmentation. Three-dimensional morphological PAM and depth-resolved images before and after PDT treatment were acquired. In addition, five quantitative vasculature parameters, including the PA signal, vessel diameter, vessel density, perfused vessel density, and vessel complexity, were analyzed to evaluate the influence of PDT on four different areas: Two melanoma tumors, and control and normal vessel areas. The quantitative and qualitative results successfully demonstrated the potential of the proposed PAM-based quantitative approach to evaluate the effectiveness of the PDT method.read more
Citations
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Journal ArticleDOI
Photodynamic Therapy—An Up-to-Date Review
TL;DR: A comprehensive path is proposed, starting with the photodynamic therapy mechanism, evolution over the years, integration of nanotechnology, and ending with a detailed review of the most important applications of this therapeutic approach.
Journal ArticleDOI
Full-view in vivo skin and blood vessels profile segmentation in photoacoustic imaging based on deep learning
TL;DR: Wang et al. as discussed by the authors proposed a DL model based on modified U-Net for extracting the relationship features between amplitudes of the generated photoacoustic signal from skin and underlying vessels.
Journal ArticleDOI
Full-view in vivo skin and blood vessels profile segmentation in photoacoustic imaging based on deep learning.
Cao Duong Ly,Van Tu Nguyen,Tan Hung Vo,Sudip Mondal,Sumin Park,Jaeyeop Choi,Thi Thu Ha Vu,Chang-Seok Kim,Junghwan Oh +8 more
TL;DR: Wang et al. as discussed by the authors proposed a DL model based on modified U-Net for extracting the relationship features between amplitudes of the generated photoacoustic signal from skin and underlying vessels.
Journal ArticleDOI
Accelerating skin regeneration and wound healing by controlled ROS from photodynamic treatment
Khatereh Khorsandi,Reza Hosseinzadeh,H. Esfahani,Kavosh Zandsalimi,Fedora Khatibi Shahidi,Heidi Abrahamse +5 more
TL;DR: In this article , a review summarizes the functions of reactive oxygen species (ROS) in living systems and describes role of photodynamic therapy in production of controllable ROS and finally a special focus on current ROS-generating therapeutic protocols for regeneration and wound healing.
Journal ArticleDOI
Combined Action of Hyper-Harmonized Hydroxylated Fullerene Water Complex and Hyperpolarized Light Leads to Melanoma Cell Reprogramming In Vitro
Milica Markelic,Dijana Drača,Tamara Krajnović,Z. Jovic,Milica Vuksanović,Djuro Koruga,Sanja Mijatović,Danijela Maksimović-Ivanić +7 more
TL;DR: Overall, 3HFWC/HPL promoted melanoma cell reprogramming toward a normal phenotype and solved the limitations of the poor solubility and bioavailability of fullerenes.
References
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Photodynamic therapy for cancer
TL;DR: PDT is being tested in the clinic for use in oncology — to treat cancers of the head and neck, brain, lung, pancreas, intraperitoneal cavity, breast, prostate and skin.
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