In vivo synthesis of complex N-glycans by expression of human N-acetylglucosaminyltransferase I in the filamentous fungus Trichoderma reesei.
Marleen Maras,André De Bruyn,Wouter Vervecken,Jaana Uusitalo,Merja Penttilä,Roger Busson,Piet Herdewijn,Roland Contreras +7 more
Reads0
Chats0
TLDR
The human N‐acetylglucosaminyltransferase I gene was introduced in the genome of Trichoderma reesei strain VTT‐D‐80133 and successful in vivo transfer of GlcNAc was demonstrated by analyzing the neutral N‐glycans which were synthesized on cellobiohydrolase I.About:
This article is published in FEBS Letters.The article was published on 1999-06-11 and is currently open access. It has received 57 citations till now. The article focuses on the topics: Trichoderma reesei.read more
Citations
More filters
Journal ArticleDOI
Trichoderma reesei RUT-C30--thirty years of strain improvement.
Robyn Peterson,Helena Nevalainen +1 more
TL;DR: The results from biochemical-, microscopic-, genomic-, transcriptomic-, glycomic- and proteomic-based research on the RUT-C30 strain published over the last 30 years are discussed.
Patent
Methods for producing modified glycoproteins
TL;DR: In this article, a combination of engineering and/or selection of strains which do not express certain enzymes which create the undesirable complex structures characteristic of the fungal glycoproteins, which express exogenous enzymes selected either to have optimal activity under the conditions present in the fungi where activity is desired, or which are targeted to an organelle where optimal activity is achieved.
Book ChapterDOI
Production of recombinant proteins: challenges and solutions.
TL;DR: This chapter discusses common problems in recombinant protein production, which include molecular biology techniques, as well as manipulation of the culture environment, and strategies for their solution.
Journal ArticleDOI
The secretion pathway in filamentous fungi: a biotechnological view
TL;DR: The data accumulated in recent years on the molecular basis of fungal secretion is compiled, emphasizing the relevance of these data for the biotechnological use of the fungal cell and indicating how this information has been applied in attempts to create improved production strains.
Journal ArticleDOI
Developing Aspergillus as a host for heterologous expression
TL;DR: History of the development of Aspergillus as an expression host, current state of the art and future directions are reviewed, touching on related research in other fungi when discussing the areas of greatest potential for future biotechnological applications.
References
More filters
Book
Molecular Cloning: A Laboratory Manual
TL;DR: Molecular Cloning has served as the foundation of technical expertise in labs worldwide for 30 years as mentioned in this paper and has been so popular, or so influential, that no other manual has been more widely used and influential.
Journal ArticleDOI
Rapid preparation of DNA from filamentous fungi
U. Raeder,P. Broda +1 more
TL;DR: A general, simple and inexpensive method for the isolation of DNA from filamentous fungi, starting from freeze‐dried mycelium 01–015% by weight, which allows the processing of many samples in parallel.
Book ChapterDOI
High-Resolution, 1H-Nuclear Magnetic Resonance Spectroscopy as a Tool in the Structural Analysis of Carbohydrates Related to Glycoproteins
TL;DR: This chapter presents literature data on the high-resolution, 1H-NMR spectroscopy of carbohydrates derived from glycoconjugates and discusses the results for carbohydrates related to the glycoproteins of N-glycosylic type.
Journal ArticleDOI
A versatile transformation system for the cellulolytic filamentous fungus Trichoderma reesei
TL;DR: An efficient transformation system for the cellulolytic filamentous fungus Trichoderma reesei has been developed and the efficiency of co-transformation with unselected DNA was high.
Journal ArticleDOI
Efficient production of secreted proteins by Aspergillus : progress, limitations and prospects
TL;DR: This review will focus on the use of filamentous fungi for the production of heterologous proteins, especially non-fungal, proteins and the effect of gene-fusion strategies will be reviewed.