Wouter Vervecken

TL;DR: The filamentous fungus Aspergillus niger is widely exploited by the fermentation industry for the production of enzymes and organic acids, particularly citric acid, and the sequenced genome revealed a large number of major facilitator superfamily transporters and fungal zinc binuclear cluster transcription factors.

TL;DR: Genomic comparison with B. thetaiotaomicron in conjunction with cell culture studies show that a cohort of highly successful members of the microbiota has evolved to consume sterically-restricted yeast glycans, an adaptation that may reflect the incorporation of eukaryotic microorganisms into the human diet.

TL;DR: Here, a protocol for engineering the N-glycosylation pathway of the yeast Pichia pastoris is provided and three examples of the full engineering procedure are provided to illustrate the results that can be expected.

TL;DR: The procedures which are developed allow glycan engineering of any P. pastoris expression strain and can yield up to 90% homogeneous protein-linked oligosaccharides, which are rapidly cleared from the blood because of the presence of terminal mannose residues.

TL;DR: It is demonstrated that UDP-galactopyranose mutase, a key enzyme of galactofuranose metabolism, controls the biosynthesis ofgalactomannan and galact ofuranose containing glycoconjugates, and appears to be an appealing adjunct therapeutic target in combination with other drugs against A. fumigatus.