Journal ArticleDOI
A versatile transformation system for the cellulolytic filamentous fungus Trichoderma reesei
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TLDR
An efficient transformation system for the cellulolytic filamentous fungus Trichoderma reesei has been developed and the efficiency of co-transformation with unselected DNA was high.About:
This article is published in Gene.The article was published on 1987-01-01. It has received 729 citations till now. The article focuses on the topics: Trichoderma reesei & Aspergillus nidulans.read more
Citations
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Journal ArticleDOI
The biological degradation of cellulose
Pierre Béguin,Jean-Paul Aubert +1 more
TL;DR: The study of cellulolytic enzymes at the molecular level has revealed some of the features that contribute to their activity and an increasing number of three-dimensional structures are becoming available for cellulases and xylanases belonging to different families, which will provide paradigms for molecular modeling of related enzymes.
Journal ArticleDOI
A versatile toolkit for high throughput functional genomics with Trichoderma reesei
André Schuster,André Schuster,Kenneth S. Bruno,James R. Collett,Scott E. Baker,Bernhard Seiboth,Christian P. Kubicek,Monika Schmoll +7 more
TL;DR: A construction kit for gene knock out in T. reesei and a primer database for gene deletion using the pyr4, amdS and hph selection markers are provided to boost research towards efficient exploitation of the resources of T.Reesei for cellulase expression and hence second generation biofuel production.
Journal ArticleDOI
Biology and biotechnology of Trichoderma
André Schuster,Monika Schmoll +1 more
TL;DR: This review aims to give a broad overview on the qualities and versatility of the best studied Trichoderma species and to highlight intriguing findings as well as promising applications.
Journal ArticleDOI
Swollenin, a Trichoderma reesei protein with sequence similarity to the plant expansins, exhibits disruption activity on cellulosic materials
Markku Saloheimo,Marja Paloheimo,Satu Hakola,Jaakko Pere,Swanson Barbara A,Eini Nyyssönen,Amit Bhatia,Michael Ward,Merja Penttilä +8 more
TL;DR: A novel gene with sequence similarity to plant expansins, isolated from the cellulolytic fungus Trichoderma reesei, is described, which has an N-terminal fungal type cellulose binding domain connected by a linker region to the expansin-like domain.
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Trichoderma reesei RUT-C30--thirty years of strain improvement.
Robyn Peterson,Helena Nevalainen +1 more
TL;DR: The results from biochemical-, microscopic-, genomic-, transcriptomic-, glycomic- and proteomic-based research on the RUT-C30 strain published over the last 30 years are discussed.
References
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Book
Molecular Cloning: A Laboratory Manual
TL;DR: Molecular Cloning has served as the foundation of technical expertise in labs worldwide for 30 years as mentioned in this paper and has been so popular, or so influential, that no other manual has been more widely used and influential.
Journal ArticleDOI
Rapid preparation of DNA from filamentous fungi
U. Raeder,P. Broda +1 more
TL;DR: A general, simple and inexpensive method for the isolation of DNA from filamentous fungi, starting from freeze‐dried mycelium 01–015% by weight, which allows the processing of many samples in parallel.
Journal ArticleDOI
Transformation of Aspergillus nidulans by using a trpC plasmid.
TL;DR: A chimeric plasmid carrying a complete copy of the trifunctional trpC gene from the Ascomycete fungus Aspergillus nidulans is constructed and replicates in Escherichia coli, where it confers resistance to ampicillin and chloramphenicol and complementstrpC mutants lacking phosphoribosylanthranilate isomerase activity.
Journal ArticleDOI
Transformation by integration in Aspergillus nidulans.
J Tilburn,Claudio Scazzocchio,Graham G. Taylor,J H Zabicky-Zissman,Robin A. Lockington,R.W. Davies +5 more
TL;DR: DNA-mediated genetic transformation of Aspergillus nidulans has been achieved by incubating protoplasts from a strain of A. niduans carrying a deletion in the acetamidase structural gene with DNA of derivatives of plasmid pBR322 containing the cloned structural gene for acetamids.
Journal ArticleDOI
Transformation of Aspergillus niger by the amdS gene of Aspergillus nidulans.
Joan M. Kelly,Michael J. Hynes +1 more
TL;DR: A transformation system is developed for Aspergillus niger using the amdS gene as a dominant heterologous marker for selecting transformants on the basis of acetamide utilization and it is shown that an unselected plasmid can be co‐transformed with the amdR/intA plasmids into A. niger.
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