Book ChapterDOI
Increase of Productivity in Recombinant Cho-Cells by Enhanced Glucose-Levels
Jürgen Fieder,P. Schorn,R. Bux,W. Noé +3 more
- pp 163-167
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TLDR
The effects of various compounds on the specific productivity of serum-free growing recombinant CHO cells are investigated, with special regard to the effects of the carbohydrate sources.Abstract:
Mammalian cells are the preferred production organisms which can produce recombinant proteins in their native, fully glycosylated form. However, the overall cellular productivity is limited, when compared to yeasts and procaryotes. Therefore the main target in bioprocess development for mammalian cell cultures is the enhancement of product yield to achieve economical and competitive product titers. In the outlined presentation we investigated the effects of various compounds on the specific productivity of serum-free growing recombinant CHO cells, with special regard to the effects of the carbohydrate sources. A strictly proportional expressed product formation was observed when compared to the glucose consumption of the corresponding cells. On the other hand, the glutamine metabolism seems to be reduced during enhanced product formation phases. The growth rate was ‘inverse’ regulated depending on the availability of the main carbohydrate (glucose).read more
Citations
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Journal ArticleDOI
Strategies for fed-batch cultivation of t-PA producing CHO cells: substitution of glucose and glutamine and rational design of culture medium.
TL;DR: A strategy for fed-batch cultivation of t-PA producing recombinant CHO cells is presented, based on the substitution of glucose and glutamine for slowly metabolized nutrients and in a rational design of the medium, that altogether increased viability, longevity and t- PA production when compared with a reference batch culture.
Journal ArticleDOI
A quantitative proteomic analysis of cellular responses to high glucose media in Chinese hamster ovary cells.
Zhenke Liu,Shujia Dai,Jonathan Bones,Somak Ray,Sangwon Cha,Barry L. Karger,Jingyi Jessica Li,Lee Wilson,Greg Hinckle,Anthony Rossomando +9 more
TL;DR: The high glucose environment may enhance recombinant dihydrofolate reductase in CHO cells by up‐regulating NCK1 and down‐ Regulating PRKRA, and may lower integrated viable cell density by activating mitochondrial‐ and endoplasmic reticulum‐mediated cell death pathways by up-regulating HtrA2 and calpains.
Book ChapterDOI
Fed-Batch Culture Development Based on Biomass Monitoring
TL;DR: The glucose concentration in the growth medium influences specific glucose consumption rate; however, a glucose limitation well below 1.1 mM is needed to modify the specific growth rate or the lactate production rate.
References
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Journal ArticleDOI
Glucose-limited chemostat culture of Chinese hamster ovary cells producing recombinant human interferon-gamma.
Paul M. Hayter,Elisabeth M. A. Curling,Anthony J. Baines,Nigel Jenkins,Ian Salmon,Phillip G. Strange,Jeremy M. Tong,Alan T. Bull +7 more
TL;DR: The proportion of fully glycosylated IFN‐γ produced by these cells was less than that produced in the early stages of batch cultures, and increased during transient periods of glucose excess, suggesting that the culture environment influences the glycoslyation of IFN-γ.
Book
Animal cell technology : products of today, prospects for tomorrow
TL;DR: Chairman's statement Making and selecting recombinant cell lines cell line characterisation complex media formulations Protein and / or serum free cultures.
Journal ArticleDOI
The enhancement of specific antibody production rate in glucose- and glutamine-controlled fed-batch culture.
TL;DR: It was found that only glutamine enrichment enhanced the specific antibody production rate, and the specific growth rate decreased with increase in glutamine concentration in the range larger than 20 mmol·1−1.
Journal ArticleDOI
Physiological changes during the adaptation of hybridoma cells to low serum and serum-free media.
TL;DR: An improvement in cell growth was observed after adaptation, and both higher growth rates and higher cell concentrations were obtained, and changes in the specific antibody production rate of the two cell lines differed.
Book ChapterDOI
Batch Kinetic Data of Hybridoma Growth and Productivity as a Basis for Simulation of Antibody Production in Different Culture Systems
TL;DR: Kinetic data from batch cultivations of a hybridoma cell line are used to generate a kinetic model describing growth and productivity of this cell line, and the usefulness of simulations in advance to extended experiments in different culture systems is demonstrated.