Induced β-Carotene Synthesis Driven by Triacylglycerol Deposition in the Unicellular Alga Dunaliella bardawil
TLDR
It is concluded that under normal conditions the carotenogenic pathway is not maximally active and may be appreciably stimulated in the presence of sequestering structures, creating a plastid-localized sink for the end product of theCarotenoid biosynthetic pathway.Abstract:
Under stress conditions such as high light intensity or nutrient starvation, cells of the unicellular alga Dunaliella bardawil overproduce β-carotene, which is accumulated in the plastids in newly formed triacylglycerol droplets. We report here that the formation of these sequestering structures and β-carotene are interdependent. When the synthesis of triacylglycerol is blocked, the overproduction of β-carotene is also inhibited. During overproduction of β-carotene no up-regulation of phytoene synthase or phytoene desaturase is observed on the transcriptional or translational level, whereas at the same time acetyl-CoA carboxylase, the key regulatory enzyme of acyl lipid biosynthesis, is increased, at least in its enzymatic activity. We conclude that under normal conditions the carotenogenic pathway is not maximally active and may be appreciably stimulated in the presence of sequestering structures, creating a plastid-localized sink for the end product of the carotenoid biosynthetic pathway.read more
Citations
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Microalgal triacylglycerols as feedstocks for biofuel production: perspectives and advances
Qiang Hu,Milton Sommerfeld,Eric E. Jarvis,Maria L. Ghirardi,Matthew C. Posewitz,Michael Seibert,Aldis Darzins +6 more
TL;DR: A brief summary of the current knowledge on oleaginous algae and their fatty acid and TAG biosynthesis, algal model systems and genomic approaches to a better understanding of TAG production, and a historical perspective and path forward for microalgae-based biofuel research and commercialization are provided.
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Jacqueline Ann Mary Paine,Shipton Catherine Ann,Sunandha Chaggar,Rhian M. Howells,Mike J Kennedy,Gareth Vernon,Susan Y Wright,Edward Hinchliffe,Jessica L Adams,Aron Louis Silverstone,Rachel Drake +10 more
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References
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