Journal ArticleDOI
Induction of Th1-type cytokines by lipoteichoic acid-related preparation isolated from OK-432, a penicillin-killed streptococcal agent.
Masato Okamoto,Go Ohe,Tetsuya Oshikawa,Hidetomo Nishikawa,Sachiko Furuichi,Hideo Yoshida,Takashi Matsuno,Motoo Saito,Mitsunobu Sato +8 more
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TLDR
Findings clearly indicated that OK-PSA, an LTA-related molecule, is a main effective component of OK-432, and is a potent inducer of Th1-type cytokines by T cell and natural killer (NK) cell activation mediated by monocytes-derived IL-18, and that it may be a useful immunotherapeutic agent for the patients with malignancies better than original Ok-432.Citations
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Dendritic cells stimulated with a bacterial product, OK-432, efficiently induce cytotoxic T lymphocytes specific to tumor rejection peptide
TL;DR: The results strongly suggest that penicillin-killed Streptococcus pyogenes (OK-432) could be applied to develop an efficient cancer vaccine using DCs pulsed with tumor rejection peptides.
Journal ArticleDOI
Expression of Toll-Like Receptor 4 on Dendritic Cells Is Significant for Anticancer Effect of Dendritic Cell-Based Immunotherapy in Combination with an Active Component of OK-432, a Streptococcal Preparation
Masato Okamoto,Sachiko Furuichi,Yasuhiko Nishioka,Tetsuya Oshikawa,Tomoyuki Tano,Sharif Uddin Ahmed,Kiyoshi Takeda,Shizuo Akira,Yoshiki Ryoma,Yoichiro Moriya,Motoo Saito,Saburo Sone,Mitsunobu Sato +12 more
TL;DR: It is suggested that OK-PSA may be a potent adjuvant for local DC therapy, and that DC therapy followed by OK- PSA is able to elicit anticancer activity even in aTLR4-deficient host when TLR4 is expressed only in DCs injected intratumorally.
Journal Article
Toll-like receptor signaling in anti-cancer immunity.
Masato Okamoto,Mitsunobu Sato +1 more
TL;DR: It is demonstrated that TLR4 signaling is intimately involved in anti-cancer immunity induced by these immunopotentiators, and the clinical examination in oral cancer patients also suggests the requirement of bothTLR4 and MD-2 in the OK-432-induced anti- cancer host response.
Journal ArticleDOI
Involvement of Toll-Like Receptor 4 Signaling in Interferon-γ Production and Antitumor Effect by Streptococcal Agent OK-432
Masato Okamoto,Tetsuya Oshikawa,Tomoyuki Tano,Go Ohe,Sachiko Furuichi,Hidetomo Nishikawa,Sharif Uddin Ahmed,Sachiko Akashi,Kensuke Miyake,Osamu Takeuchi,Shizuo Akira,Yoichiro Moriya,Shuzo Matsubara,Yoshiki Ryoma,Motoo Saito,Mitsunobu Sato +15 more
TL;DR: TLR4 and MD-2 may mediate OK-432-induced anticancer immunity and be associated with the in vivo IFN-gamma induction in 19 patients administeredOK-432.
Journal ArticleDOI
Mechanism of anticancer host response induced by OK-432, a streptococcal preparation, mediated by phagocytosis and Toll-like receptor 4 signaling.
Masato Okamoto,Tetsuya Oshikawa,Tomoyuki Tano,Sharif Uddin Ahmed,Shin Kan,Akiko Sasai,Sachiko Akashi,Kensuke Miyake,Yoichiro Moriya,Yoshiki Ryoma,Motoo Saito,Mitsunobu Sato +11 more
TL;DR: In in vivo experiments, the anticancer effect of OK-432 was significantly inhibited by suppression of phagocytosis activity by cytochalasin B, and in vitro experiments elucidated the mechanism mediated by phagocytes and TLR4 signaling in the immune effect ofOK-432.
References
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Journal ArticleDOI
Single-step method of RNA isolation by acid guanidinium thiocyanate-phenol-chloroform extraction
TL;DR: A new method of total RNA isolation by a single extraction with an acid guanidinium thiocyanate-phenol-chloroform mixture is described, providing a pure preparation of undegraded RNA in high yield and can be completed within 4 h.
Journal Article
Two types of murine helper T cell clone. I. Definition according to profiles of lymphokine activities and secreted proteins.
TL;DR: A panel of antigen-specific mouse helper T cell clones was characterized according to patterns of lymphokine activity production, and two types of T cell were distinguished.
Journal ArticleDOI
Cloning of a new cytokine that induces IFN-gamma production by T cells.
Haruki Okamura,H Tsutsi,T Komatsu,M Yutsudo,A Hakura,T Tanimoto,K Torigoe,T Okura,Y Nukada,K Hattori +9 more
TL;DR: The cloning of a recently identified IFN-γ-inducing factor (IGIF) that augments natural killer activity in spleen cells and may be involved in the development of Thl cells and also in mechanisms of tissue injury in inflammatory reactions is reported.
Journal ArticleDOI
Identification and purification of natural killer cell stimulatory factor (NKSF), a cytokine with multiple biologic effects on human lymphocytes.
M Kobayashi,Lori Fitz,M Ryan,Rodney M. Hewick,Steven C. Clark,S Chan,R Loudon,F Sherman,B Perussia,Giorgio Trinchieri +9 more
TL;DR: Data strongly suggest that the same molecule mediates these three activities, although the presence of traces of contaminant peptides even in the most purified NKSF preparations does not allow us to exclude the possibility that distinct biologically active molecules have been co-purified.