Influence of soil properties on fluorescein diacetate hydrolyzing activity under different agro-ecosystems
Abstract: Fluorescein diacetate hydrolyzing activity (FDHA) of soils, both in field moist and air dried, of different agro-ecosystems in alluvial soil was studied. Field moist soils recorded higher values than in air-dried soils, but they showed significant positive correlation (0.93∗∗). FDHA in both soil pretreatments showed positive correlation with organic matter (0.98∗∗ for field moist soil and 0.92∗∗ for air-dried soil) and clay content (0.85∗∗ for field moist soil and 0.82∗ for air-dried soil) of the soils. Multiple regression analysis showed that the stabilization of FDHA in the studied soils was due to organic matter-enzyme complex. Among the organic matter fractions, humus C plays dominating role to control the FDHA of the soils.
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...…activities involve in decomposition of soil organic matter, such as non-specific esterases, proteases, and lipases activities) was assayed using methods as described by Chakrabarti and Bhattacharyya (2006) on 1 g of soil sample mixed with 60 mM sodium phosphate buffer (pH 7.6) and 5.0 mM FDA....
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...Available phosphorus (P) and potassium (K) were estimated according to Black (1965)....
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...Organic carbon (C) was determined by wet digestion method as described by Nelson and Sommers (1982) and total nitrogen (N) by micro-Kjeldahl method as described by Sankaram (1966)....
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...The pH was determined in a 1 : 2.5 soil : water suspension while sand, silt and clay percentages were measured by the International Pipette Method (Piper 1966)....
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