Journal ArticleDOI
Inhibition of DNA-dependent RNA polymerase reaction of Escherichia coll by an antimicrobial antibiotic, streptovaricin
TLDR
The effect of an antimicrobial antibiotic, streptovaricin, was studied on DNA-dependent RNA-synthetic reaction by RNA polymerase which was extracted from Ehrlich ascites carcinoma cells in a soluble state and showed no inhibition of the reaction.About:
This article is published in Biochimica et Biophysica Acta.The article was published on 1968-04-22. It has received 66 citations till now. The article focuses on the topics: Streptovaricin & RNA-dependent RNA polymerase.read more
Citations
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Journal ArticleDOI
Interaction of rifamycin with bacterial RNA polymerase.
Journal ArticleDOI
Actions of the rifamycins.
W Wehrli,M Staehelin +1 more
TL;DR: Rifamycins as a Tool in Biochemical Studies and mode of Interaction Between RifamyCins and Bacterial RNA Polymerase, and the nature of the enzyme-rifamycin complex.
Journal ArticleDOI
Rifamycins--obstacles and opportunities.
TL;DR: It is suggested that it is quite possible to devise improved rifamycin analogs, and improved activity against rifampin-resistant strains by some analogs promises that further work in this area, especially if the information from co-crystal structures with RNA polymerase is applied, should lead to even better analogs.
Journal ArticleDOI
Inhibition of dna-dependent rna synthesis by rifamycins
TL;DR: The results indicate that the incorporation of 3H-CMP into acid insoluble fraction by RNA polymerase of E. coli was markedly inhibited by both anti biotics at a concentration as low as 0.1 jum.
Journal ArticleDOI
A rifampicin resistent rna-polymerase from E. coli altered in the β-subunit
D. Rabussay,Wolfram Zillig +1 more
TL;DR: Evidence is presented indicating that rifampicin may interact with subunit 0, which therefore should play a role in initiation of RNA synthesis, and appears to act on an initiation step which precedes the formation of the first internucleotide bond.
References
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Journal Article
Protein Measurement with the Folin Phenol Reagent
TL;DR: Procedures are described for measuring protein in solution or after precipitation with acids or other agents, and for the determination of as little as 0.2 gamma of protein.
Journal ArticleDOI
A procedure for the isolation of deoxyribonucleic acid from micro-organisms
TL;DR: A method has been described for the isolation of DNA from micro-organisms which yields stable, biologically active, highly polymerized preparations relatively free from protein and RNA, and Representative samples have been characterized for their thermal stability and sedimentation behaviour.
Journal ArticleDOI
The Determination of Enzyme Dissociation Constants
Hans Lineweaver,Dean Burk +1 more
TL;DR: On the basis of the assumed theory the rate of the observed reaction is directly proportional to the concentration of the enzyme-substrate compound, where (E:l = (ES).
Journal ArticleDOI
Base Specificity in the Interaction of Polynucleotides with Antibiotic Drugs
TL;DR: Chromomycin and olivomycin form complexes with DNA, preferably in the helical form, but not with RNA, and none of the other antibiotics requires the presence of any single base in the template for its action.