Insecticidal crystal proteins of Bacillus thuringiensis.
Herman Höfte,H R Whiteley +1 more
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TLDR
In this article, a classification for crystal protein genes of Bacillus thuringiensis is presented, based on the insecticidal spectra and the amino acid sequences of the encoded proteins.About:
This article is published in Microbiological Research.The article was published on 1989-06-01 and is currently open access. It has received 2044 citations till now. The article focuses on the topics: Delta endotoxin & Bacillus thuringiensis.read more
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Journal ArticleDOI
Quantification of the effect of Bacillus thuringiensis toxins on short-circuit current in the midgut of Bombyx mori
TL;DR: Two Bacillus thuringiensis insecticidal toxins, CryIAa and CryIAc, prepared from Escherichia coli-cloned gene products, are examined to inhibit short-circuit current in midgut epithelia of Bombyx mori, showing a correlation between toxin concentration and inhibitory response.
Journal ArticleDOI
Transgenic rice plants expressing a modified cry1Ca1 gene are resistant to Spodoptera litura and Chilo suppressalis.
Mohsin Abbas Zaidi,Mohsin Abbas Zaidi,Gongyin Ye,Hongwei Yao,Taek H. You,Taek H. You,Evelin Loit,Donald H. Dean,Sheikh Riazuddin,Illimar Altosaar +9 more
TL;DR: Analysis of R1 transgenic plants indicated that the cry1Ca1 was inherited by the progeny plants and provided complete protection against C. suppressalis and S.litura larvae as revealed by insect bioassays.
Book ChapterDOI
Bacillus thuringiensis endotoxins: action on the insect midgut
TL;DR: In this paper, the authors summarize the current knowledge on the interactions of Bacillus thuringiensis toxins with the insect midgut epithelium, discussing the various methodologies applied to study these interactions and their limitations.
Journal ArticleDOI
cry Genes profiling and the toxicity of isolates of Bacillus thuringiensis from soil samples against American bollworm, Helicoverpa armigera
TL;DR: The aim of this study was to search for Bacillus thuringiensis harbouring cry1A gene which could effectively control cotton pest, American bollworm, Helicoverpa armigera.
Journal ArticleDOI
Characterization of a cry2A gene cloned from an isolate of Bacillus thuringiensis serovar sotto.
Jun Sasaki,Shin-ichiro Asano,Naoki Hashimoto,Bibiana W. Lay,Sugyo Hastowo,Hisanori Bando,Toshihiko Iizuka +6 more
TL;DR: Cry2(SKW), a cry2A-type gene, was cloned from Bacillus thuringiensis serovar sotto SKW01-10.2-06 and was less toxic to a lepidopteran species, Bomberx mori, than Cry2Aa in spite of high homology between the two proteins.
References
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Analysis of membrane and surface protein sequences with the hydrophobic moment plot.
TL;DR: An algorithm has been developed which identifies alpha-helices involved in the interactions of membrane proteins with lipid bilayers and which distinguishes them from helices in soluble proteins, and suggests four transmembrane helices and a surface-seeking helix in fragment B, the moiety known to have trans Membrane function.
Journal ArticleDOI
Transgenic plants protected from insect attack
Mark Vaeck,Arlette Reynaerts,Herman Höfte,Stefan Jansens,Marc De Beuckeleer,Caroline Dean,Caroline Dean,Marc Zabeau,Marc Van Montagu,Jan Leemans +9 more
TL;DR: The Gram-positive bacterium Bacillus thuringiensis produces proteins which are specifically toxic to a variety of insect species, and modified genes have been derived from bt2, a toxin gene cloned from one Bacillus strain.
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Stable Transformation of Soybean (Glycine Max) by Particle Acceleration
TL;DR: Experiments using this protocol have resulted in mature RO and R1 plants expressing the introduced gene(s), regarded as a major step in the practical application of genetic engineering to the useful modification of food-crop species.
Journal ArticleDOI
Insect Tolerant Transgenic Tomato Plants
David A. Fischhoff,Katherine S. Bowdish,Katherine S. Bowdish,Frederick J. Perlak,Pamela G. Marrone,Sheila McCormick,Sheila McCormick,Jeanne G. Niedermeyer,Duff A. Dean,Kuniko Kusano-Kretzmer,Ernest J. Mayer,Dean E. Rochester,Stephen G. Rogers,Robert T. Fraley +13 more
TL;DR: The structure of an insect control protein gene from Bacillus thuringiensis var.
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Bacillus thuringiensis var israelensis crystal delta-endotoxin: effects on insect and mammalian cells in vitro and in vivo
Wendy E. Thomas,David J. Ellar +1 more
TL;DR: With the exception of the Lepidopteran cell line, Choristoneura fumiferana 63 CF1, this soluble crystal delta-endotoxin protein showed no in vitro or in vivo toxicity, and no haemolytic activity.