Insecticidal crystal proteins of Bacillus thuringiensis.
Herman Höfte,H R Whiteley +1 more
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TLDR
In this article, a classification for crystal protein genes of Bacillus thuringiensis is presented, based on the insecticidal spectra and the amino acid sequences of the encoded proteins.About:
This article is published in Microbiological Research.The article was published on 1989-06-01 and is currently open access. It has received 2044 citations till now. The article focuses on the topics: Delta endotoxin & Bacillus thuringiensis.read more
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Journal ArticleDOI
The solubility of inclusion proteins from Bacillus thuringiensis is dependent upon protoxin composition and is a factor in toxicity to insects.
TL;DR: It was found that a higher pH was required to solubilize protoxins from inclusions from the plasmid-cured strain than from B. thuringiensis subsp.
Journal ArticleDOI
Prospects for reducing fumonisin contamination of maize through genetic modification.
TL;DR: Transgenic approaches to ear mold/mycotoxin resistance are now feasible as well, and could provide further protection for the grower in environments where FBs present a risk to the crop even when the maize is relatively resistant to Fusarium mold.
Journal ArticleDOI
On the fate of ingested Bacillus spores.
Maria R Spinosa,Tiziana Braccini,Ezio Ricca,Maurilio De Felice,Lorenzo Morelli,Gianni Pozzi,Marco R. Oggioni +6 more
TL;DR: It is indicated that Bacillus is present in the intestinal tract solely as spores and that nonpathogenic Bacillus spores may germinate in lymphoid organs, a finding reminiscent of B. anthracis germination in macrophages.
Book ChapterDOI
Compartmentalization of digestion
TL;DR: Evidence for this spatial arrangement of digestion in the midgut is reviewed and the importance of an evolutionary or phylogenetic approach to an understanding of the many convergent and divergent morphological and biochemical features responsible for digestion in insects is stressed.
Journal ArticleDOI
Toxicity of Bacillus thuringiensis Spore and Crystal Protein to Resistant Diamondback Moth (Plutella xylostella).
Juliet D. Tang,Anthony M. Shelton,J. Van Rie,S De Roeck,William J. Moar,Richard T. Roush,M Peferoen +6 more
TL;DR: A colony of Plutella xylostella from crucifer fields in Florida was used in mortality bioassays and revealed high levels of field-evolved resistance to HD-1 spore and all CryIA protoxins and no resistance to CryIB, CryIC, or CryID.
References
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Analysis of membrane and surface protein sequences with the hydrophobic moment plot.
TL;DR: An algorithm has been developed which identifies alpha-helices involved in the interactions of membrane proteins with lipid bilayers and which distinguishes them from helices in soluble proteins, and suggests four transmembrane helices and a surface-seeking helix in fragment B, the moiety known to have trans Membrane function.
Journal ArticleDOI
Transgenic plants protected from insect attack
Mark Vaeck,Arlette Reynaerts,Herman Höfte,Stefan Jansens,Marc De Beuckeleer,Caroline Dean,Caroline Dean,Marc Zabeau,Marc Van Montagu,Jan Leemans +9 more
TL;DR: The Gram-positive bacterium Bacillus thuringiensis produces proteins which are specifically toxic to a variety of insect species, and modified genes have been derived from bt2, a toxin gene cloned from one Bacillus strain.
Journal ArticleDOI
Stable Transformation of Soybean (Glycine Max) by Particle Acceleration
TL;DR: Experiments using this protocol have resulted in mature RO and R1 plants expressing the introduced gene(s), regarded as a major step in the practical application of genetic engineering to the useful modification of food-crop species.
Journal ArticleDOI
Insect Tolerant Transgenic Tomato Plants
David A. Fischhoff,Katherine S. Bowdish,Katherine S. Bowdish,Frederick J. Perlak,Pamela G. Marrone,Sheila McCormick,Sheila McCormick,Jeanne G. Niedermeyer,Duff A. Dean,Kuniko Kusano-Kretzmer,Ernest J. Mayer,Dean E. Rochester,Stephen G. Rogers,Robert T. Fraley +13 more
TL;DR: The structure of an insect control protein gene from Bacillus thuringiensis var.
Journal ArticleDOI
Bacillus thuringiensis var israelensis crystal delta-endotoxin: effects on insect and mammalian cells in vitro and in vivo
Wendy E. Thomas,David J. Ellar +1 more
TL;DR: With the exception of the Lepidopteran cell line, Choristoneura fumiferana 63 CF1, this soluble crystal delta-endotoxin protein showed no in vitro or in vivo toxicity, and no haemolytic activity.