Insecticidal crystal proteins of Bacillus thuringiensis.
Herman Höfte,H R Whiteley +1 more
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TLDR
In this article, a classification for crystal protein genes of Bacillus thuringiensis is presented, based on the insecticidal spectra and the amino acid sequences of the encoded proteins.About:
This article is published in Microbiological Research.The article was published on 1989-06-01 and is currently open access. It has received 2044 citations till now. The article focuses on the topics: Delta endotoxin & Bacillus thuringiensis.read more
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Maximal toxicity of cloned CytA δ-endotoxin from Bacillus thuringiensis subsp. israelensis requires proteolytic processing from both the N- and C-termini
TL;DR: In vitro cytolyticAssays against Aedes aegypti cells, and haemolytic assays against rat erythrocytes, showed that CytA processed at both termini by proteinase K was the most active form and this processing enhances the toxicity in vitro and possibly in vivo.
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Midgut GPI-anchored proteins with alkaline phosphatase activity from the cotton boll weevil (Anthonomus grandis) are putative receptors for the Cry1B protein of Bacillus thuringiensis
Érica Soares Martins,Rose Gomes Monnerat,Paulo Roberto Queiroz,Vinicius Fiuza Dumas,Shélida Vasconcelos Braz,Raimundo Wagner de Souza Aguiar,Ana Cristina M. M. Gomes,Jorge Sánchez,Alejandra Bravo,Bergmann Morais Ribeiro +9 more
TL;DR: This work is the first report that shows the localization of Cry toxin receptors in the midgut cells of A. grandis, and it is found that Cry1Ba6 binds to two proteins that showed alkaline phosphatase (ALP) activity.
Journal ArticleDOI
Molecular cloning of the 130-kilodalton mosquitocidal delta-endotoxin gene of Bacillus thuringiensis subsp. israelensis in Bacillus sphaericus
TL;DR: A 3.7-kilobase (kb) XbaI fragment harboring the cryIVB gene was found to be present by agarose gel electrophoresis and Southern blot hybridization and produced a 130-kDa mosquitocidal toxin which was detected by Western (immuno-) blot analysis with antibody prepared against B. thuringiensis subsp.
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Parasporin-2 from a New Bacillus thuringiensis 4R2 Strain Induces Caspases Activation and Apoptosis in Human Cancer Cells
TL;DR: It is found that proteinase K activated parasporin-2Aa1 protein isolated from a novel B. thuringiensis strain, 4R2, was specifically cytotoxic to endometrial, colon, liver, cervix, breast and prostate cancer and showed no toxicity against normal cells.
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Expression of a Bacillus thuringiensisdelta-endotoxin cry1Ab gene in Bacillus subtilis and Bacillus licheniformis strains that naturally colonize the phylloplane of tomato plants (Lycopersicon esculentum, Mills).
TL;DR: Modified microorganisms capable of surviving on leaf surfaces for several weeks with insecticidal activity should allow for a reduction in pesticide application.
References
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Analysis of membrane and surface protein sequences with the hydrophobic moment plot.
TL;DR: An algorithm has been developed which identifies alpha-helices involved in the interactions of membrane proteins with lipid bilayers and which distinguishes them from helices in soluble proteins, and suggests four transmembrane helices and a surface-seeking helix in fragment B, the moiety known to have trans Membrane function.
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Transgenic plants protected from insect attack
Mark Vaeck,Arlette Reynaerts,Herman Höfte,Stefan Jansens,Marc De Beuckeleer,Caroline Dean,Caroline Dean,Marc Zabeau,Marc Van Montagu,Jan Leemans +9 more
TL;DR: The Gram-positive bacterium Bacillus thuringiensis produces proteins which are specifically toxic to a variety of insect species, and modified genes have been derived from bt2, a toxin gene cloned from one Bacillus strain.
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Stable Transformation of Soybean (Glycine Max) by Particle Acceleration
TL;DR: Experiments using this protocol have resulted in mature RO and R1 plants expressing the introduced gene(s), regarded as a major step in the practical application of genetic engineering to the useful modification of food-crop species.
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Insect Tolerant Transgenic Tomato Plants
David A. Fischhoff,Katherine S. Bowdish,Katherine S. Bowdish,Frederick J. Perlak,Pamela G. Marrone,Sheila McCormick,Sheila McCormick,Jeanne G. Niedermeyer,Duff A. Dean,Kuniko Kusano-Kretzmer,Ernest J. Mayer,Dean E. Rochester,Stephen G. Rogers,Robert T. Fraley +13 more
TL;DR: The structure of an insect control protein gene from Bacillus thuringiensis var.
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Bacillus thuringiensis var israelensis crystal delta-endotoxin: effects on insect and mammalian cells in vitro and in vivo
Wendy E. Thomas,David J. Ellar +1 more
TL;DR: With the exception of the Lepidopteran cell line, Choristoneura fumiferana 63 CF1, this soluble crystal delta-endotoxin protein showed no in vitro or in vivo toxicity, and no haemolytic activity.